Characterization of long-term mouse brain aggregating cultures: Evidence for maintenance of neural precursor cells

2004 ◽  
Vol 474 (2) ◽  
pp. 246-260 ◽  
Author(s):  
C. Mikaela D. Berglund ◽  
Johan Aarum ◽  
Samantha L. Budd Haeberlein ◽  
Jens R. Nyengaard ◽  
Tomas Hökfelt ◽  
...  
Keyword(s):  
Mouse Brain ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor

scholarly journals Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells

2005 ◽  
Vol 95 (5) ◽  
pp. 1311-1320 ◽  
Author(s):  
Makoto Yanagisawa ◽  
Tetsuya Taga ◽  
Kazuo Nakamura ◽  
Toshio Ariga ◽  
Robert K. Yu
Keyword(s):  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor

scholarly journals TAT-Hsp70 Induces Neuroprotection Against Stroke Via Anti-Inflammatory Actions Providing Appropriate Cellular Microenvironment for Transplantation of Neural Precursor Cells

2013 ◽  
Vol 33 (11) ◽  
pp. 1778-1788 ◽  
Author(s):  
Thorsten R Doeppner ◽  
Britta Kaltwasser ◽  
Jin Fengyan ◽  
Dirk M Hermann ◽  
Mathias Bähr
Keyword(s):  
Cerebral Ischemia ◽  
Nuclear Translocation ◽  
Infarct Volume ◽  
Underlying Mechanism ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Paracrine Effects ◽  
Extracellular Milieu

Heat-shock protein 70 (Hsp70) protects against cerebral ischemia, which is attributed to its chaperone activity. However, recent reports also describe pro-inflammatory actions of Hsp70 via activation of Toll-like receptors (TLR). Using membrane-permeable transactivator of transcription (TAT)-Hsp70, we analyzed TAT-Hsp70-induced neuroprotection and its underlying mechanism after cerebral ischemia in mice. Infusion of TAT-Hsp70 reduced infarct volume and enhanced blood–brain barrier integrity on day 3 poststroke, when given no later than 12 hours. The latter was associated with reduction of microglial activation, although upregulation of pro-inflammatory TLR-2/4 was observed both in verum and in control animals. Nevertheless, protein abundance and nuclear translocation of downstream nuclear factor kappa B (NF-κB) as well as proteasomal degradation of the NF-κB regulator Ikappa B alpha (IκB-α) were significantly reduced by TAT-Hsp70. TAT-Hsp70-induced neuroprotection and functional recovery were restricted to 4 weeks only. However, TAT-Hsp70 provided an appropriate extracellular milieu for delayed intravenous transplantation of adult neural precursor cells (NPCs). Thus, NPCs that were grafted 28 days poststroke induced long-term neuroprotection for at least 3 months, which was not due to integration of grafted cells but rather due to paracrine effects of transplanted NPCs. Conclusively, TAT-Hsp70 ameliorates postischemic inflammation via proteasome inhibition, thus providing an appropriate extracellular milieu for delayed NPC transplantation and culminating in long-term neuroprotection.

scholarly journals Macroporous heparin-based microcarriers allow long-term 3D culture and differentiation of neural precursor cells

Biomaterials ◽  
2020 ◽  
Vol 230 ◽  
pp. 119540 ◽  
Author(s):  
Ben Newland ◽  
Fanny Ehret ◽  
Franziska Hoppe ◽  
Dimitri Eigel ◽  
Dagmar Pette ◽  
...  
Keyword(s):  
3D Culture ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor

Long-Term Proliferation and Dopaminergic Differentiation of Human Mesencephalic Neural Precursor Cells

2001 ◽  
Vol 170 (2) ◽  
pp. 317-325 ◽  
Author(s):  
Alexander Storch ◽  
Gesine Paul ◽  
Marie Csete ◽  
Bernhard O. Boehm ◽  
Paul M. Carvey ◽  
...  
Keyword(s):  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Dopaminergic Differentiation

scholarly journals Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments

1992 ◽  
Vol 103 (2) ◽  
pp. 589-597 ◽  
Author(s):  
J. Dahlstrand ◽  
L.B. Zimmerman ◽  
R.D. McKay ◽  
U. Lendahl
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Intermediate Filaments ◽  
Intermediate Filament ◽  
Evolutionary Relationship ◽  
Neuronal Cell ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor

Multipotential stem cells in the neural tube give rise to the different neuronal cell types found in the brain. Abrupt changes in intermediate filament gene expression accompany this transition out of the precursor state: transcription of the intermediate filament nestin is replaced by that of the neurofilaments. In order to identify human neural precursor cells, and to learn more about the evolution of the intermediate filaments expressed in the central nervous system, we have isolated the human nestin gene. Despite considerable divergence between the human and rat nestin genes, in particular in the repetitive parts of the carboxy-terminal region, the positions of the introns are perfectly conserved. Two of the three intron positions are also shared by the neurofilaments, but not by other classes of intermediate filaments. This implies that nestin and the neurofilaments had a common ancestor after branching off from the other classes of intermediate filaments, and that nestin separated from the neurofilament branch before the different neurofilament genes diverged. The characterization of human nestin also facilitates the identification of human multipotential neural precursor cells. This will be of importance for central nervous system (CNS) tumor diagnosis and transplant-based clinical approaches to human neurodegenerative diseases.

Isolation and characterization of mouse neural precursor cells in primary culture

1991 ◽  
Vol 27 (8) ◽  
pp. 615-624 ◽  
Author(s):  
Hiroshi Kitani ◽  
Robert Shiurba ◽  
Teruyo Sakakura ◽  
Yasuhiro Tomooka
Keyword(s):  
Primary Culture ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Isolation And Characterization

scholarly journals Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Pia S. Jensen ◽  
Lise Lyck ◽  
Pia Jensen ◽  
Jens Zimmer ◽  
Morten Meyer
Keyword(s):  
Growth Factor ◽  
Dopaminergic Neurons ◽  
Neural Tissue ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Large Animal ◽  
Ventral Mesencephalic

The potential use of predifferentiated neural precursor cells for treatment of a neurological disorder like Parkinson’s disease combines stem cell research with previous experimental and clinical transplantation of developing dopaminergic neurons. One current obstacle is, however, the lack of ability to generate dopaminergic neurons after long-termin vitropropagation of the cells. The domestic pig is considered a useful nonprimate large animal model in neuroscience, because of a better resemblance of the larger gyrencephalic pig brain to the human brain than the commonly used brains of smaller rodents. In the present study, porcine embryonic (28–30 days), ventral mesencephalic precursor cells were isolated and propagated as free-floating neural tissue spheres in medium containing epidermal growth factor and fibroblast growth factor 2. For passaging, the tissue spheres were cut into quarters, avoiding mechanical or enzymatic dissociation in order to minimize cellular trauma and preserve intercellular contacts. Spheres were propagated for up to 237 days with analysis of cellular content and differentiation at various time points. Our study provides the first demonstration that porcine ventral mesencephalic precursor cells can be long-term propagated as neural tissue spheres, thereby providing an experimental 3Din vitromodel for studies of neural precursor cells, their niche, and differentiation capacity.

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