Distribution of acidic fibroblast growth factor mRNA-expressing neurons in the adult mouse central nervous system

1995 ◽  
Vol 359 (2) ◽  
pp. 323-339 ◽  
Author(s):  
Adelheid Kresse ◽  
Ralf Pettersson ◽  
Tomas Hökfelt
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Adult Mouse ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor ◽  
Mouse Central Nervous System

Central nervous system distribution of fibroblast growth factor injected into the blood stream

1996 ◽  
Vol 18 (3) ◽  
pp. 267-272 ◽  
Author(s):  
Pedro Cuevas ◽  
Argentina Fernández-Ayerdi ◽  
Fernando Carceller ◽  
Silvie Colin ◽  
Frédéric Mascarelli ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Blood Stream ◽  
Fibroblast Growth

scholarly journals Acidic Fibroblast Growth Factor is Present in the Enteric Nervous System of the Large Intestine

2000 ◽  
Vol 48 (3) ◽  
pp. 407-413 ◽  
Author(s):  
Anthony Capetandes ◽  
Jerry Di Salvo ◽  
John J. Ronan ◽  
Kenneth A. Thomas
Keyword(s):  
Nervous System ◽  
Growth Factor ◽  
Enteric Nervous System ◽  
Fibroblast Growth Factor ◽  
Large Intestine ◽  
Myenteric Plexus ◽  
Immunohistochemical Analysis ◽  
Fibroblast Growth ◽  
Heparin Binding ◽  
Acidic Fibroblast Growth Factor

Acidic fibroblast growth factor (aFGF) is a heparin binding protein that displays pleiotropic activity. The purpose of this study was to document the presence of the translated aFGF product, its mRNA, and its location in the colon. mRNA was extracted from bovine large intestine and reverse transcribed to cDNA. Nested-primer PCR was used to determine the presence of mRNA using primers homologous to the previously published bovine aFGF cDNA. Purification of translated aFGF was performed using an established HPLC protocol. Western blot analysis of the HPLC fractions was performed using two epitopeindependent antibodies against aFGF. Immunohistochemistry employed these antibodies to determine the locus of aFGF expression. The nested-primer PCR product of predicted size was homologous to the published bovine aFGF mRNA sequence, as determined by DNA sequencing. Intestinal aFGF had a mass similar to bovine aFGF isolated from other tissues, and immunocrossreacted with two peptide-based, epitope-independent anti-aFGF antisera on Western blotting. Immunohistochemical analysis of large intestine using these two independent antisera localized aFGF within the myenteric plexus. These data demonstrate that aFGF is present within the myenteric plexus of the enteric nervous system.

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