scholarly journals Dissecting the Biology of the Fungal Wheat Pathogen Zymoseptoria tritici : A Laboratory Workflow

2020 ◽  
Vol 59 (1) ◽  
Author(s):  
Wagner C. Fagundes ◽  
Janine Haueisen ◽  
Eva H. Stukenbrock
Keyword(s):  
Zymoseptoria Tritici ◽  
Laboratory Workflow

scholarly journals Improved molecular laboratory productivity by consolidation of testing on the new random-access analyzer Alinity m

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Martin Obermeier ◽  
Monia Pacenti ◽  
Robert Ehret ◽  
Francesco Onelia ◽  
Rory Gunson ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Random Access ◽  
Mycoplasma Genitalium ◽  
Transmitted Infection ◽  
Sexually Transmitted ◽  
B Virus ◽  
Immunodeficiency Virus ◽  
International Multicenter Study ◽  
Laboratory Productivity ◽  
Laboratory Workflow

AbstractObjectivesAutomated molecular analyzers have accelerated diagnosis, allowing earlier intervention and better patient follow-up. A recently developed completely automated molecular analyzer, Alinity™ m (Abbott), offers consolidated, continuous, and random-access testing that may improve molecular laboratory workflow.MethodsAn international, multicenter study compared laboratory workflow metrics across various routine analyzers and Alinity m utilizing assays for human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV), hepatitis B virus (HBV), high-risk human papillomavirus (HR HPV), and sexually transmitted infection (STI) (Chlamydia trachomatis [CT]/Neisseria gonorrhoeae [NG]/Trichomonas vaginalis [TV]/Mycoplasma genitalium [MG]). Three turnaround times (TATs) were assessed: total TAT (sample arrival to result), sample onboard TAT (sample loading and test starting to result), and processing TAT (sample aspiration to result).ResultsTotal TAT was reduced from days with routine analyzers to hours with Alinity m, independent of requested assays. Sample onboard TATs for standard workflow using routine analyzers ranged from 7 to 32.5 h compared to 2.75–6 h for Alinity m. The mean sample onboard TAT for STAT samples on Alinity m was 2.36 h (±0.19 h). Processing TATs for Alinity m were independent of the combination of assays, with 100% of results reported within 117 min.ConclusionsThe consolidated, continuous, random-access workflow of Alinity m reduces TATs across various assays and is expected to improve both laboratory operational efficiency and patient care.

Sesamol‐based terpenoids as promising bio‐sourced crop protection compounds against the wheat pathogen Zymoseptoria tritici

2021 ◽  
Author(s):  
Audrey Damiens ◽  
Mohammad Taghi Alebrahim ◽  
Estelle Léonard ◽  
Antoine Fayeulle ◽  
Christophe Furman ◽  
...  
Keyword(s):  
Crop Protection ◽  
Zymoseptoria Tritici

γ‐Lactam‐Based Antifungal Compounds against the Wheat Pathogen Zymoseptoria tritici

2021 ◽  
Author(s):  
Audrey Damiens ◽  
Anca‐Elena Dascalu ◽  
Mohammad Taghi Alebrahim ◽  
Christophe Furman ◽  
Emmanuelle Lipka ◽  
...  
Keyword(s):  
Antifungal Compounds ◽  
Zymoseptoria Tritici

Correlation of fungal penetration, CWDE activities and defense-related genes with resistance of durum wheat cultivars to Zymoseptoria tritici

2017 ◽  
Vol 100 ◽  
pp. 117-125 ◽  
Author(s):  
L. Somai-Jemmali ◽  
A. Siah ◽  
K. Harbaoui ◽  
S. Fergaoui ◽  
B. Randoux ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Wheat Cultivars ◽  
Zymoseptoria Tritici

scholarly journals Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici

2017 ◽  
Vol 217 (1) ◽  
pp. 320-331 ◽  
Author(s):  
Graeme J. Kettles ◽  
Carlos Bayon ◽  
Caroline A. Sparks ◽  
Gail Canning ◽  
Kostya Kanyuka ◽  
...  
Keyword(s):  
Zymoseptoria Tritici

scholarly journals Characterisation of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici

2017 ◽  
Author(s):  
Graeme J. Kettles ◽  
Carlos Bayon ◽  
Caroline A. Sparks ◽  
Gail Canning ◽  
Kostya Kanyuka ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Effector Proteins ◽  
Yeast Cells ◽  
Septoria Tritici Blotch ◽  
Septoria Tritici ◽  
List Type ◽  
Expression Systems ◽  
Zymoseptoria Tritici ◽  
Loop Region

Abstract-The fungus Zymoseptoria tritici is the causal agent of Septoria Tritici Blotch (STB) disease of wheat leaves. Z. tritici secretes many functionally uncharacterised effector proteins during infection. Here we characterised a secreted ribonuclease (Zt6) with an unusual biphasic expression pattern.-Transient expression systems were used to characterise Zt6, and mutants thereof, in both host and non-host plants. Cell-free protein expression systems monitored impact of Zt6 protein on functional ribosomes, and in vitro assays of cells treated with recombinant Zt6 determined toxicity against bacteria, yeasts and filamentous fungi.-We demonstrated that Zt6 is a functional ribonuclease and that phytotoxicity is dependent on both the presence of a 22-amino acid N-terminal “loop” region and its catalytic activity. Zt6 selectively cleaves both plant and animal rRNA species, and is toxic to wheat, tobacco, bacterial and yeast cells but not to Z. tritici itself.-Zt6 is the first Z. tritici effector demonstrated to have a likely dual functionality. The expression pattern of Zt6 and potent toxicity towards microorganisms suggests that whilst it may contribute to the execution of wheat cell death, it is also likely to have an important secondary function in antimicrobial competition and niche protection.

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