An All-Inclusive Laboratory Workflow for the Development of Surfactant/Polymer Formulations for EOR in Harsh Carbonates

Carbonate reservoirs are challenging for chemical EOR, particularly in selecting fine-tuned chemical formulations which combine high performance, stable behavior, and trouble-free operations. The design of suitable formulations requires substantial laboratory work and a solid methodology. In this paper, a systematic all-inclusive laboratory workflow to design a surfactant-polymer (SP) formulation for a carbonate reservoir is presented. In this work, a complete process for development and evaluation of an SP formulation for high-salinity high-temperature conditions is proposed and adopted. For which, a high throughput robotic platform is used for efficient and robust formulation design. The process is illustrated on an actual case with harsh reservoir conditions (i.e. a high temperature of 100℃ and high connate salinity of 213,000 mg/L). The SP design methodology consisted of five steps: surfactant design, polymer selection, surfactant/polymer verification, topside assessment, and oil-displacement evaluation. The surfactant formulation design consisted of four substeps: solubility scans, phase-behavior scans (salinity scans), IFT measurements, and static adsorption tests. The sourced polymers were screened based on three key performance indicators: viscosity, filter ratio, and thermal stability. The selected surfactant formulations and polymers were then assessed as sloppy slugs in terms of compatibility and injectivity. Then, the unique topside assessment was conducted where it consisted of two components focusing on: separation kinetics and separated water quality. Finally, an oil displacement study was performed using a preserved composite plug, in which the SP formulation developed through the outlined process was used. The results demonstrate the potential of a mixture of Olefin Sulfonate (OS) and Alkyl Glyceryl Ether Sulfonate (AGES). The results also illustrate couple of polymers with stabilities suitable for high temperature conditions: an associative polymer, and an AM/AMPS copolymer. In addition, injectivity corefloods supported the SP slug transportability across the porous media. Corefloods also demonstrated the SP slug capacity to recover around 62% ROIC (remaining oil in core). Finally, SP in produced brines improved the separation kinetics but lead to a slight deterioration in separated water quality. A key novelty of the adopted workflow is the integration of topside assessment. In addition, the experimental steps were clearly delineated including the preparation of representative oils. Beside a clear layout of the methodology, the work demonstrates that a surfactant-polymer formulation can successfully be designed for high temperature carbonate reservoirs and provide encouraging guidelines with respect to SP impact on topside facilities.

The Use of Static Telecytological Applications for Proficiency Testing Purposes in the Field of Diagnostic Cytopathology

Over the last decade, the practice of clinical cytopathology was dramatically influenced by the wide implementation of informatics and computer sciences into the laboratory workflow. New types of cameras and microscopes connected to computers made possible image capture and transmission (telecytology). Remote cytological diagnosis can nowadays be achieved either with the use of cytological pictures viewed in real-time from the microscope (dynamic telecytological systems) or with the use of cytological pictures that are first captured in a digital format and then transmitted using a store-and-forward approach to distant observers (static telecytological systems). ISO 15189:2012 for medical laboratories requires successful participation in proficiency testing programs. This article emphasizes on the feasibility of using static telecytological applications in order to implement a proficiency test for cytopathology labs wishing to be accredited according to ISO 15189:2012.

An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates

Marine invertebrates are model organisms in several areas of biological sciences, being a source of massive biological information. Although, the scientific relevance of marine invertebrates, the research with them can be limited for their tissue characteristics and troubles for the replication of physical and chemical properties of seawater. Thence, the main goal of this laboratory workflow is to provide a useful methodological approach to reduce the experimental limitations during the study of marine invertebrates. The present study describes experimental methodologies for the collection, transport, and maintenance of sessile tunicates. Also, an approach to observe and characterize, a diverse population of blood cells in marine invertebrates, by several cytological stains and electron microscopy. Lastly, suggestions and protocols to extract quality RNA from samples with high concentrations of salts, pigments, secondary metabolites, and polysaccharides. This methodological approach can be easily adapted to other marine invertebrates, moreover uses low-cost reagents and widely available laboratory equipment. Making possible the study of different types of marine animals in diverse locations.

The MTB/MDR ELITe MGB® Kit: Performance Assessment for Pulmonary, Extra-Pulmonary, and Resistant Tuberculosis Diagnosis, and Integration in the Laboratory Workflow of a French Center

A rapid and reliable diagnostic for tuberculosis, including the detection of both rifampicin (RIF) and isoniazid (INH) resistance, is essential for appropriate patient care. Nucleic acid amplification tests are a fast alternative to methods based on Mycobacterium tuberculosis complex (MTB) cultures. Thus, the performance of the MDR/MTB ELITe MGB® Kit on the ELITe InGenius® platform was retrospectively evaluated for MTB detection on pulmonary and extra-pulmonary samples and for RIF/INH resistance detection on MTB strains. The sensitivity and specificity of the kit for MTB detection compared to the MTB culture were 80.0% and 100.0%, respectively. For the antimicrobial susceptibility prediction, the agreement with phenotypic antimicrobial susceptibility testing (AST) was 92.0%. For RIF, the sensitivity was 100.0% and the specificity was 95.5%. For INH, the sensitivity and specificity were 75.0% and 100.0%, respectively. A single RIF false-positive result was obtained for a strain with a low level of RIF resistance that was not detected by phenotypic AST, but carrying a rpoB L452P mutation. INH false-negative results (3) were due to mutations on the katG gene that were not probed by the test. Overall, the MTB/MDR ELITe MGB® Kit presents a strong performance for MTB detection and for the detection of both RIF and INH resistance, with an easy integration in laboratory workflow thanks to its fully automatized system.

Performance of digital morphology analyzer Vision Pro on white blood cell differentials

Objectives Vision Pro (West Medica, Perchtoldsdorf, Austria) is a recently developed digital morphology analyzer. We evaluated the performance of Vision Pro on white blood cell (WBC) differentials. Methods In a total of 200 peripheral blood smear samples (100 normal and 100 abnormal samples), WBC preclassification and reclassification by Vision Pro were evaluated and compared with manual WBC count, according to the Clinical and Laboratory Standards Institute guidelines (H20-A2). Results The overall sensitivity was high for normal WBCs and nRBCs (80.1–98.0%). The overall specificity and overall efficiency were high for all cell classes (98.1–100.0% and 97.7–99.9%, respectively). The absolute values of mean differences between Vision Pro and manual count ranged from 0.01 to 1.31. In leukopenic samples, those values ranged from 0.09 to 2.01. For normal WBCs, Vision Pro preclassification and manual count showed moderate or high correlations (r=0.52–0.88) except for basophils (r=0.34); after reclassification, the correlation between Vision Pro and manual count was improved (r=0.36–0.90). Conclusions This is the first study that evaluated the performance of Vision Pro on WBC differentials. Vision Pro showed reliable analytical performance on WBC differentials with improvement after reclassification. Vision Pro could help improve laboratory workflow.

Improved molecular laboratory productivity by consolidation of testing on the new random-access analyzer Alinity m

ObjectivesAutomated molecular analyzers have accelerated diagnosis, allowing earlier intervention and better patient follow-up. A recently developed completely automated molecular analyzer, Alinity™ m (Abbott), offers consolidated, continuous, and random-access testing that may improve molecular laboratory workflow.MethodsAn international, multicenter study compared laboratory workflow metrics across various routine analyzers and Alinity m utilizing assays for human immunodeficiency virus type 1 (HIV-1), hepatitis C virus (HCV), hepatitis B virus (HBV), high-risk human papillomavirus (HR HPV), and sexually transmitted infection (STI) (Chlamydia trachomatis [CT]/Neisseria gonorrhoeae [NG]/Trichomonas vaginalis [TV]/Mycoplasma genitalium [MG]). Three turnaround times (TATs) were assessed: total TAT (sample arrival to result), sample onboard TAT (sample loading and test starting to result), and processing TAT (sample aspiration to result).ResultsTotal TAT was reduced from days with routine analyzers to hours with Alinity m, independent of requested assays. Sample onboard TATs for standard workflow using routine analyzers ranged from 7 to 32.5 h compared to 2.75–6 h for Alinity m. The mean sample onboard TAT for STAT samples on Alinity m was 2.36 h (±0.19 h). Processing TATs for Alinity m were independent of the combination of assays, with 100% of results reported within 117 min.ConclusionsThe consolidated, continuous, random-access workflow of Alinity m reduces TATs across various assays and is expected to improve both laboratory operational efficiency and patient care.