An Efficient Method for DNA Purification‐Free PCR from Plant Tissue

AbstrakProtokol dan metode sederhana, efisien untuk isolasi DNA genom tanaman durian yang banyak mengandung phenol dan residu polisakarida telah dihasilkan. Pada penelitian ini, digunakan protokol isolasi DNA tumbuhan dengan metode CTAB yang dimodifikasi sebagai protokol yang efisien untuk membuang polisakarida, phenol dan lendir yang sangat melimpah pada tanaman durian. Obyek penelitian ini terdiri dari protocol CTAB yang dimodifikasi tahap inkubasi dan presipitasi pemurnian DNA genom dari phenol dan polisakarida. Perbandingan 2 protokol isolasi DNA durian dengan CTAB standard an CTAB modifikasi menunjukkan bahwa metode CTAB modifikasi menghasilkan whole genom durian cukup murni rata rata 1,99 dan berhasil diamplifikasi dengan PCR-RAPD.Kata kunci: isolasi, DNA, polisakarida, CTAB, modifikasi, . AbstractThe simple and efficient method for genomic DNA isolation protochol from durian , its woody fruit crops containing high polysaccharide levels has been described here. In the present study, using modified CTAB for plant DNA isolation protocols were studied for removing the highly concentrated polysaccharides from genomic DNA of woody fruit crops.This method involves the modified CTAB at the incubate and precipitate procedure employing DNA purification step to remove polysaccharides and phenol residu. Compared with the two studied DNA isolation protocols of durian using standart CTAB and modified CTAB the everage yield high quality DNA whole genom is 1,99 purity and DNA was suitable for PCR and RAPD analyses.Keyword: isolation, DNA, polysaccharides, phenol residu, CTAB

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DNA Purification-free PCR from Plant Tissue

Plant and Cell Physiology ◽

10.1093/pcp/pcab120 ◽

2021 ◽

Author(s):

Zhengnan Jia ◽

Miaomiao Ding ◽

Masahito Nakano ◽

Kunqi Hong ◽

Ruidong Huang ◽

...

Keyword(s):

Plant Tissue ◽

Dna Purification

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Freeze-fracture, freeze-etch complementary pairs of virus-infected cells reveal value of etching even when relatively high concentrations of cryoprotectants are used

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081541 ◽

1978 ◽

Vol 36 (2) ◽

pp. 136-137

Author(s):

Russell L. Steere ◽

Eric F. Erbe

Keyword(s):

Plant Tissue ◽

Phosphate Buffer ◽

Infected Plant ◽

Freeze Fracture ◽

Distilled Water ◽

Virus Infected Plant ◽

Infected Cells ◽

High Concentrations

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.

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Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002798 ◽

1980 ◽

Vol 38 ◽

pp. 636-637

Author(s):

R. D. Sjolund ◽

C. Y. Shih

Keyword(s):

Plant Tissue ◽

Cultured Cells ◽

Freeze Fracture ◽

Tissue Cultures ◽

Sieve Elements ◽

Intact Plants ◽

Plant Tissue Cultures ◽

Whole Plants ◽

Energy Dependent ◽

Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

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