A Simple and Efficient Method for DNA Purification from Samples of Highly Clotted Blood

2010 ◽  
Vol 46 (3) ◽  
pp. 258-264 ◽  
Author(s):  
Ruyi Xu ◽  
Ping Ye ◽  
Leiming Luo ◽  
Hongmei Wu ◽  
Jin Dong ◽  
...  
Keyword(s):  
Efficient Method ◽  
Dna Purification

scholarly journals Pengembangan Protokol Isolasi DNA Genom Tanaman Durian Dengan Menggunakan Modifikasi Bufer CTAB

2018 ◽  
Vol 6 (02) ◽  
pp. 33
Author(s):  
Sundari Sundari
Keyword(s):  
Efficient Method ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Dna Purification ◽  
Fruit Crops ◽  
Purification Step ◽  
High Quality ◽  
Plant Dna ◽  
Genomic Dna Isolation

AbstrakProtokol dan metode sederhana, efisien untuk isolasi  DNA genom tanaman durian   yang banyak mengandung phenol dan residu polisakarida telah dihasilkan. Pada penelitian ini, digunakan  protokol isolasi DNA tumbuhan  dengan metode CTAB yang dimodifikasi sebagai protokol yang  efisien untuk membuang polisakarida, phenol  dan  lendir yang sangat melimpah pada tanaman durian. Obyek penelitian ini  terdiri dari protocol CTAB yang dimodifikasi  tahap inkubasi dan presipitasi  pemurnian DNA genom dari phenol dan polisakarida. Perbandingan 2  protokol  isolasi  DNA durian dengan CTAB standard an CTAB modifikasi  menunjukkan bahwa metode  CTAB modifikasi menghasilkan  whole genom durian cukup murni rata rata 1,99 dan berhasil diamplifikasi  dengan PCR-RAPD.Kata kunci:  isolasi, DNA, polisakarida, CTAB, modifikasi, . AbstractThe simple and efficient  method for genomic DNA isolation protochol from durian , its woody fruit crops containing high polysaccharide levels has been described here. In the present study, using modified CTAB for plant DNA isolation protocols were studied for removing the highly concentrated polysaccharides from genomic DNA of woody fruit crops.This method involves the modified CTAB   at the incubate  and precipitate procedure employing DNA purification  step to remove polysaccharides and phenol residu.  Compared with the two  studied DNA  isolation protocols of  durian using standart CTAB and modified CTAB  the everage yield  high quality DNA whole genom is 1,99 purity and DNA was suitable for PCR and RAPD analyses.Keyword: isolation, DNA,  polysaccharides,  phenol residu, CTAB

An Efficient Method for DNA Purification‐Free PCR from Plant Tissue

2021 ◽  
Vol 1 (11) ◽  
Author(s):  
Zhengnan Jia ◽  
Xiaowei Han ◽  
Kenichi Tsuda
Keyword(s):  
Efficient Method ◽  
Plant Tissue ◽  
Dna Purification

An Efficient Method for Estimating Aerodynamic Angles for Small Unmanned Aircraft

2019 ◽  
Author(s):  
Guilherme Barufaldi ◽  
Marcus Victor ◽  
Luiz Carlos Sandoval Góes ◽  
ROBERTO GIL ANNES DA SILVA
Keyword(s):  
Efficient Method ◽  
Unmanned Aircraft ◽  
Aerodynamic Angles

Speed vs. comfort: Testing the efficacy of a single question for predicting transit users’ route choices

2019 ◽  
Author(s):  
Joseph John Pyne Simons ◽  
Ilya Farber
Keyword(s):  
Data Collection ◽  
Efficient Method ◽  
Choice Task ◽  
The Self ◽  
Self Report ◽  
Model Parameters ◽  
Single Item ◽  
Alternative Approach ◽  
Single Question ◽  
Insight Into

Not all transit users have the same preferences when making route decisions. Understanding the factors driving this heterogeneity enables better tailoring of policies, interventions, and messaging. However, existing methods for assessing these factors require extensive data collection. Here we present an alternative approach - an easily-administered single item measure of overall preference for speed versus comfort. Scores on the self-report item predict decisions in a choice task and account for a proportion of the differences in model parameters between people (n=298). This single item can easily be included on existing travel surveys, and provides an efficient method to both anticipate the choices of users and gain more general insight into their preferences.

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