scholarly journals Bivalent response to long-term storage in liquid-preserved boar semen: A flow cytometric analysis

2012 ◽  
Vol 81A (7) ◽  
pp. 576-587 ◽  
Author(s):  
Heiko Henning ◽  
Anna M. Petrunkina ◽  
Robin A. P. Harrison ◽  
Dagmar Waberski
Keyword(s):  
Flow Cytometric Analysis ◽  
Long Term Storage ◽  
Flow Cytometric ◽  
Boar Semen ◽  
Term Storage

scholarly journals Cryopreservation of boar semen

2020 ◽  
Vol 65 (No. 4) ◽  
pp. 115-123
Author(s):  
Marija Jovičić ◽  
Eva Chmelíková ◽  
Markéta Sedmíková
Keyword(s):  
Animal Species ◽  
Semen Quality ◽  
Egg Yolk ◽  
High Rate ◽  
Freezing And Thawing ◽  
Long Term Storage ◽  
Boar Semen ◽  
Boar Spermatozoa ◽  
Term Storage

Sperm cryopreservation is the best technology for long-term storage of the semen. However, the damage of boar spermatozoa by cryopreservation is more severe than in other animal species and a standardized freezing protocol for efficient cryopreservation has not been established yet. Semen quality and freezability vary greatly between breeds as well as between individual boars and even the season. Boar spermatozoa are sensitive to low temperatures; they sustain damage and a high rate of mortality and freezing/thawing the boar semen may strongly impair the sperm function and decrease the semen quality. The freezability of boar semen can be influenced by a cryopreservation procedure, and also by using various additives to freezing and thawing extenders such as antioxidants. In order to obtain acceptable results after thawing the boar semen, it is necessary to combine an optimal amount of additives (glycerol, egg yolk, sugars, antioxidants), cooling and warming velocities.

scholarly journals Temperature Effects on Interspecific Hybridization between Gladiolus ×grandiflora and G. tristis

HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 341-343 ◽  
Author(s):  
Yasumasa Takatsu ◽  
Masakazu Kasumi ◽  
Toru Manabe ◽  
Mikio Hayashi ◽  
Eiichi Inoue ◽  
...  
Keyword(s):  
Interspecific Hybridization ◽  
Wild Species ◽  
Storage Temperature ◽  
Pollen Viability ◽  
Flow Cytometric Analysis ◽  
Morphological Data ◽  
Modern Cultivar ◽  
Long Term Storage ◽  
Flow Cytometric

Interspecific hybridization between a modern cultivar of Gladiolu×grandiflora hort. (2n = 60) and the wild species G. tristis L. (2n = 30) was made to introduce characteristics of the wild species into the cultivated one. Gladiolus ×grandiflora is a summer-flowering species, and G. tristis flowers in winter. The effect of storage temperature on pollen viability was tested, as long-term storage of pollen was necessary to facilitate crossing these two species. Pollen of G. tristis could be stored at -20 °C for ≈1 year, and was more practical than storage at -80 °C. Air temperature affected pollen tube growth, fertility, and fruit set in the cross between G. ×grandiflora and G. tristis, and low temperatures (15 to 20 °C) were best. The morphological data and flow cytometric analysis showed that the F1 plants were hybrids between G. ×grandiflora and G. tristis.

Adenosine monophosphate-activated kinase, AMPK, is involved in the maintenance of the quality of extended boar semen during long-term storage

2013 ◽  
Vol 80 (4) ◽  
pp. 285-294 ◽  
Author(s):  
David Martin-Hidalgo ◽  
Ana Hurtado de Llera ◽  
Marc Yeste ◽  
M. Cruz Gil ◽  
M. Julia Bragado ◽  
...  
Keyword(s):  
Adenosine Monophosphate ◽  
Long Term Storage ◽  
Boar Semen ◽  
Term Storage

Inter- and intra-breed comparative study of sperm motility and viability in Iberian and Duroc boar semen during long-term storage in MR-A and XCell extenders

2013 ◽  
Vol 139 (1-4) ◽  
pp. 109-114 ◽  
Author(s):  
D. Martín-Hidalgo ◽  
F.J. Barón ◽  
A. Robina ◽  
M.J. Bragado ◽  
A. Hurtado de Llera ◽  
...  
Keyword(s):  
Comparative Study ◽  
Sperm Motility ◽  
Long Term Storage ◽  
Boar Semen ◽  
Term Storage

scholarly journals 212EFFECT OF CRYOPRESERVATION METHODS AND PRE-CRYOPRESERVATION STORAGE ON BOTTLENOSE DOLPHIN (TURSIOPS TRUNCATES) SPERMATOZOA

2004 ◽  
Vol 16 (2) ◽  
pp. 227
Author(s):  
T.R. Robeck ◽  
J.K. O¿Brien
Keyword(s):  
Bottlenose Dolphin ◽  
Bottlenose Dolphins ◽  
Sperm Concentration ◽  
Flow Cytometric Analysis ◽  
Egg Yolk ◽  
Motility Index ◽  
Long Term Storage ◽  
Flow Cytometric ◽  
Cryopreservation Techniques ◽  
Term Storage

In conjunction with artificial insemination (AI) and sperm preservation, sperm sexing technology has great potential as a population management strategy for captive bottlenose dolphins. Successful AI using fresh spermatozoa (Robeck TR et al. 2001 CRC Marine Mammal Medicine 193–226) and flow cytometric analysis of bottlenose dolphin spermatozoa (Garner DL and Seidel GE Jr 2002 CSAS Symposium 2–13) support this approach. For sperm sexing, methods for short-term storage of semen in a liquid state are required to enable transport of spermatozoa to the sorting laboratory. In addition, cryopreservation techniques must be optimized for long-term storage of sexed spermatozoa. Our objectives were to assess: (i) 3 cryopreservation methods×2 straw sizes×3 thawing rates (Exp. 1) and (ii) effects of liquid storage for 24h (pre-cryopreservation) and sperm concentration at freezing (Exp. 2) on post-thaw characteristics (PT) of bottlenose dolphin spermatozoa. For Exp. 1 and 2, 4 ejaculates (collected by manual stimulation)×3 males (aged 14–34yr)×4 replicates were used. For Exp. 1, semen was frozen in 0.25-mL (SM) and 0.5-mL straws (LG) by 3 methods (Mt) (Mt1: lactose, egg yolk, −32°Cmin−1; Mt2: lactose, egg yolk, 1.5% Equex STM (Nova Chemical, Calgary, Canada), −19.7°Cmin−1; Mt3: Test yolk buffer (TYB), −116°Cmin−1). All Mt had 3% glycerol. Samples were thawed using a slow (S: 2.8°Cs−1), medium (M: 8.8°Cs−1) or fast (F: 21°Cs−1) rate. In Exp. 2, ejaculates were divided into 4 aliquots for dilution (1:1) and stored at 4°C with EquiPro® (EP4°C, Minitube, Verona, WI, USA) and TYB (TYB4°C) or at 21°C with Androhep EnduraguardTM (AH21°C, Minitube) or no dilution (NEAT21°C). After 24h, samples were frozen and thawed using Mt3×SM×F at 10×106 spermmL−1 (LOW) or 100×106 spermmL−1 (STD). PT evaluations of motility (total motility [TM], % progressive motility [PPM], kinetic rating [KR, 0 to 5]) and acrosomal status (Spermac® , Minitube) were performed at 30min and 6h after dilution (1:1) with AH at 21°C. For statistical analysis (ANOVA), a sperm motility index (SMI=TM×PPM×KR) was calculated and expressed as % of initial SMI. For all ejaculates, initial TM and PPM were greater than 85% and KR was 5. In Exp. 1, at 6h PT, %SMI was highest for Mt3×LG×M (45.5±8.7) and Mt3×SM×F (44.8±11.9). For Exp. 2, %SMI at 0h PT was higher for samples stored at 4°C than at 21°C (TYB4°C 41.0±8.4, EP4°C: 36.7±7.7, NEAT21°C: 23.8±8.6, AH21°C: 14.8±8.6, P<0.001) and, with the exception of AH21°C, was similar between the LOW and STD concentration. At 6h PT, %SMI for all treatments was higher for STD than LOW concentration (P<0.05). Acrosome integrity was similar across treatments. In summary, a semen cryopreservation protocol maintained high levels of the initial characteristics of ejaculated spermatozoa. Transport of semen for sex pre-selection and cryopreservation within 24h may be feasible, but impact of storage time on functional capacity of dolphin spermatozoa is unknown.

The effect of melatonin on the quality of extended boar semen after long-term storage at 17 °C

2011 ◽  
Vol 75 (8) ◽  
pp. 1550-1560 ◽  
Author(s):  
D. Martín-Hidalgo ◽  
F.J. Barón ◽  
M.J. Bragado ◽  
P. Carmona ◽  
A. Robina ◽  
...  
Keyword(s):  
Long Term Storage ◽  
Boar Semen ◽  
Term Storage
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