scholarly journals Temperature Effects on Interspecific Hybridization between Gladiolus ×grandiflora and G. tristis

HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 341-343 ◽  
Author(s):  
Yasumasa Takatsu ◽  
Masakazu Kasumi ◽  
Toru Manabe ◽  
Mikio Hayashi ◽  
Eiichi Inoue ◽  
...  

Interspecific hybridization between a modern cultivar of Gladiolu×grandiflora hort. (2n = 60) and the wild species G. tristis L. (2n = 30) was made to introduce characteristics of the wild species into the cultivated one. Gladiolus ×grandiflora is a summer-flowering species, and G. tristis flowers in winter. The effect of storage temperature on pollen viability was tested, as long-term storage of pollen was necessary to facilitate crossing these two species. Pollen of G. tristis could be stored at -20 °C for ≈1 year, and was more practical than storage at -80 °C. Air temperature affected pollen tube growth, fertility, and fruit set in the cross between G. ×grandiflora and G. tristis, and low temperatures (15 to 20 °C) were best. The morphological data and flow cytometric analysis showed that the F1 plants were hybrids between G. ×grandiflora and G. tristis.

1996 ◽  
Vol 121 (5) ◽  
pp. 804-809 ◽  
Author(s):  
I. David van der Walt ◽  
Gail M. Littlejohn

The influence of storage temperature and humidity on pollen viability was studied in four Protea species. Pollen was stored at a range of temperatures and relative humidities for up to 1 year and tested for ability to germinate in vitro. Pollen of P. repens (L.) L. `Sneyd', P. eximia (Salisb. ex Knight) Fourcade `Fiery Duchess' and P. magnifica Link. clone T 84 07 05 stored at -196 °C and -14 to -18 °C retained a germination percentage as high as that of fresh pollen regardless of humidity. Humidity control became increasingly important at storage temperatures above 0 °C. The study showed that long-term storage of Protea pollen is not feasible at temperatures above 0 °C. The relationship between germinability and fluorochromasia (FCR) was studied during storage of `Sneyd' pollen. The correlations between FCR and germinability were found to be low and nonsignificant. Fifteen-month-old cryopreserved `Sneyd' pollen functioned in fertilization and seed set as effectively as fresh pollen.


2012 ◽  
Vol 81A (7) ◽  
pp. 576-587 ◽  
Author(s):  
Heiko Henning ◽  
Anna M. Petrunkina ◽  
Robin A. P. Harrison ◽  
Dagmar Waberski

2010 ◽  
Vol 58 (2) ◽  
pp. 89 ◽  
Author(s):  
Y. Li ◽  
Y. L. Zhang ◽  
C. D. Jiang ◽  
T. Wang ◽  
Q. Wang ◽  
...  

To effectively preserve the vulnerable species of Alsophila, we studied the effects of varying the temperature and duration of storage on spore viability, early gametophyte development and the microstructure of brown spores of three Alsophila species. Spores of A. spinulosa (Wall. ex Hook.) Tryon and A. gigantea Wall. ex Hook. lost viability quickly when stored at room temperature and suffered from great loss when stored at –18°C from 6 to 12 months. Within 1 month, spore viability of A. spinulosa and A. gigantea stored at 4°C was higher than that of those stored in liquid nitrogen. In contrast, long-term storage in liquid nitrogen resulted in a comparatively small loss of viability for these two species. The spores of A. podophylla Hook. died within 3 months after storage at room temperature, 4°C and –18°C, and they died within 12 months when stored in liquid nitrogen. The spores of A. spinulosa and A. gigantea stored at room temperature, 4°C and –18°C, were prone to develop into abnormal gametophytes. These results suggest that storage of A. spinulosa and A. gigantea spores in liquid nitrogen is an effective method of preserving these vulnerable species. The reasons for the failure to preserve ephemeral A. podophylla spores by storage in liquid nitrogen are discussed.


2020 ◽  
Author(s):  
Daniel Gaudet ◽  
Narendra Singh Yadav ◽  
Aleksei Sorokin ◽  
Andrii Bilichak ◽  
Igor Kovalchuk

AbstractPollen viability and storage is of great interest to cannabis breeders and researchers to maintain desirable germplasm for future use in breeding or for biotechnological and gene editing applications. Here, we report a simple and efficient cryopreservation method for long-term storage of Cannabis sativa pollen. Additionally, we have deciphered the bicellular nature of cannabis pollen using DAPI staining. We have also standardized a pollen germination assay to assess the viability of cannabis pollen, and found pollen collected from different principal growth stages exhibits different longevity. Finally, we developed a long-term storage method which includes pollen combination with baked whole wheat flower and desiccation under vacuum for cryopreservation. By using this method, we were able to maintain germination viability in liquid nitrogen after 4 months, suggesting potentially indefinite preservation of cannabis pollen.


HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 530A-530
Author(s):  
S.M. Scheiber ◽  
C.D. Robacker ◽  
M.A. Dirr

The genus Abelia contains ≈30 species, but A. × grandiflora, its cultivars, and A. `Edward Goucher' are the primary taxa grown. The nursery industry has stated that Abelia R. Br. taxa are important economically, and new selections or cultivars with increased cold hardiness, richer pink-rose flower colors, unique foliage colors, and compact habits are desired. Breeding and selection work in the genus is very limited due in part to limited access to germplasm. Pollen storage enables breeders to cross taxa with incongruent flowering cycles, save time and resources by eliminating the need to grow vast amounts of plant material, and incorporate otherwise unavailable germplasm into a breeding program. An experiment was conducted to determine the optimum levels of temperature and humidity for the long-term storage of A. chinensis and A. × grandiflora `Golden Glow' pollen. Temperature and humidity levels were analyzed by incubating undesiccated pollen of a given taxon at four humidity levels (0%, 50%, 80%, and 100%) for 72 h at 5 °C. Following incubation, the pollen was stored in glass vials at each of the following temperatures: 5, -20, and -70 °C. All combinations of temperature and humidity were tested. Pollen viability was assessed after 60 days by in vivo germination tests on styles. Abelia chinensis pollen germinated following storage at all temperature and humidity levels. Pollen of A. × grandiflora `Golden Glow' pollen germinated following all treatments except storage at -20 °C.


Author(s):  
O.R. Davletbaeva ◽  
M.G. Ibragimbekov ◽  
A.N. Khovrin

Представлена оценка сохранности сортов, гибридов и новых гибридных комбинаций лука репчатого выращенного в однолетней культуре в условиях Московской области. Лежкость селекционных образцов лука оценивали в период зимнего хранения, учитывали убыль массы, число усохших, пораженных и проросших луковиц. По количеству сохранившихся луковиц и динамике этих показателей оценивали сохранность образцов. Сохранность лука репчатого зависит еще от пригодности данного сорта или гибрида к длительному хранению и его вызревания. Полное вызревание лука характеризуется наличием сухих кроющих чешуй, усыханием листьев и шейки, высоким содержанием сахарозы и моносахаров. Такой лук хранится дольше и меньше поражается болезнями. Один из важных способов сокращения потерь лука репчатого при хранении является соблюдение низкотемпературного режима и соответствующей влажности воздуха. Для лучшей сохранности лука поддерживали температуру в хранилище около 12 С, относительной влажностью воздуха в пределах 75-80. Для этого лук закладывали в хранилище с оборудованными холодильными установками, автоматически регулирующими температуру, влажность и циркуляцию воздуха. Большинство образцов имели сохранность от 28,3 до 71,1, что составило более половины 69,2 изучаемых образцов. Самый низкий показатель сохранности имел образец 652/17 F1 (ШетAN 158 I3) и составил 28,3, также данный образец имел 42,3 проросших луковиц. Лучшую сохранность 95 и 94,6 показали образцы зарубежной селекции F1 Сантеро и F1 Премито (стандарт), при содержании сухого вещества 10,9 и 10,8 соответственно. Также хорошую сохранность среди гибридных комбинаций показал образец 652/2 F1 (Шет Поло I3) 85 с содержанием сухого вещества 10,2, уступив отечественному сорту Форвард (стандарт) на 1,8.An assessment of the safety of cultivars, hybrids and new hybrid combinations of onion grown in an annual culture in the Moscow region is presented. The yield of selected onion samples was evaluated during winter storage, taking into account the loss of weight, the number of dried, affected and sprouted bulbs. The number of preserved bulbs and the dynamics of these indicators were used to assess the safety of samples. The safety of onions depends on the suitability of this variety or hybrid for long-term storage and its aging. Full maturation of onions is characterized by the presence of dry covering scales, drying of leaves and neck, high content of sucrose and monosaccharides. This onion is stored longer and less affected by diseases. One of the important ways to reduce the loss of onions during storage is to observe a low-temperature regime and appropriate humidity. For better preservation of onions, the storage temperature was maintained at about 1-2 C, with a relative humidity of 75-80. To do this, the onion was placed in a storage with equipped refrigeration units that automatically regulate temperature, humidity and air circulation. The majority of samples had a safety of 28.3 to 71.1, which was more than half-69.2 of the studied samples. The lowest preservation rate was for the 652/17 F1 sample (ShetAN 158 I3) and was 28.3, and this sample also had 42.3 sprouted bulbs. The best preservation of 95 and 94.6 were shown by samples of foreign selection F1 Santero and F1 Premito (standard), with a dry matter content of 10.9 and 10.8, respectively. Also, the 652/2 F1 (Shet Polo I3) sample showed good preservation among hybrid combinations 85 with a dry matter content of 10.2, losing to the domestic Forvard (standard) cultivar by 1.8.


TH Open ◽  
2020 ◽  
Vol 04 (01) ◽  
pp. e36-e39
Author(s):  
Christina Griesser ◽  
Michael Myskiw ◽  
Werner Streif

AbstractParoxysmal nocturnal hemoglobinuria (PNH) is a chronic disease caused by complement-mediated hemolysis. Clinical symptoms include intravascular hemolysis, nocturnal hemoglobinuria, thromboses, cytopenia, fatigue, abdominal pain, and a strong tendency toward bone marrow failure. It is a rare disease, especially in children, with high mortality rates without appropriate treatment.We here present the case of a 17-year-old girl with unprovoked muscle vein thrombosis. Flow cytometric analysis showed deficiency of glycosyl-phosphatidylinositol-anchored membrane proteins on all three hematopoietic cell lines and confirmed the diagnosis of PNH. Treatment with the monoclonal antibody eculizumab achieved long-term remission.As flow cytometry is normally not part of the routine diagnostics for pediatric thrombosis, awareness is crucial and PNH is important to consider in all children with thrombosis at atypical sites and abnormalities in blood counts with regard to hemolysis and cytopenia.


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