scholarly journals Environmental DNA analysis as a non-invasive quantitative tool for reproductive migration of a threatened endemic fish in rivers

2018 ◽  
Vol 8 (23) ◽  
pp. 11964-11974 ◽  
Author(s):  
Atsushi Maruyama ◽  
Kousuke Sugatani ◽  
Kazuki Watanabe ◽  
Hiroki Yamanaka ◽  
Akio Imamura
Keyword(s):  
Dna Analysis ◽  
Environmental Dna ◽  
Non Invasive ◽  
Endemic Fish ◽  
Quantitative Tool ◽  
Reproductive Migration

RELATIONSHIPS BETWEEN DISTRIBUTION PATTERNS OF Plecoglossus altivelis altivelis ESTIMATED BY ENVIRONMENTAL DNA ANALYSIS AND STREAM TEMPARATURE

2018 ◽  
Vol 74 (7) ◽  
pp. III_457-III_462
Author(s):  
Takanori KONO ◽  
Yoshihisa AKAMATSU ◽  
Ryutei INUI ◽  
Masuji GOTO ◽  
Kohei YAMAGUCHI
Keyword(s):  
Dna Analysis ◽  
Environmental Dna ◽  
Distribution Patterns ◽  
Plecoglossus Altivelis

SPAWNING HABITAT POTENTIAL EVALUATION FOR AYU BASED ON AN ENVIRONMENTAL DNA ANALYSIS AND FIELD WORKS OF PHYSICAL ENVIRONMENT FACTORS IN THE LOWER ASAHI RIVER

2019 ◽  
Vol 75 (2) ◽  
pp. I_529-I_534
Author(s):  
Keisuke YOSHIDA ◽  
Ryuutei INUI ◽  
Ryohei UDAGAWA ◽  
Shiro MAENO ◽  
Yoshihisa AKAMATSU ◽  
...  
Keyword(s):  
Physical Environment ◽  
Dna Analysis ◽  
Environmental Dna ◽  
Spawning Habitat ◽  
Environment Factors ◽  
Potential Evaluation ◽  
Field Works

ASSESSMENT ON THE SEASONAL BIOMASS VARIATIONS OF RIVERINE ORGANISMS USING ENVIRONMENTAL DNA ANALYSIS IN THE TAKATSU RIVER, WESTERN JAPAN

2020 ◽  
Vol 76 (2) ◽  
pp. I_1303-I_1308
Author(s):  
Kanta KOBAYASHI ◽  
Minoru SAITO ◽  
Yoshihisa AKAMATSU ◽  
Ryutei INUI ◽  
Takanori KONO ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Environmental Dna ◽  
Western Japan

EXAMINATION OF ENVIRONMENTAL FACTORS AFFECTING FISH ASSEMBLAGES IN THE DOWNSTREAM SEGMENT OF THE HAJI DAM USING ENVIRONMENTAL DNA ANALYSIS

2020 ◽  
Vol 76 (2) ◽  
pp. I_1309-I_1314
Author(s):  
Seiji MIYAZONO ◽  
Takao KODAMA ◽  
Yoshihisa AKAMATSU ◽  
Ryohei NAKAO ◽  
Satsuki TSUJI
Keyword(s):  
Environmental Factors ◽  
Dna Analysis ◽  
Fish Assemblages ◽  
Environmental Dna ◽  
Factors Affecting

scholarly journals Re-discovering jaguar in remaining coastal Atlantic Forest in southeastern Brazil by non-invasive DNA analysis

2017 ◽  
Vol 17 (2) ◽  
Author(s):  
Andiara Silos Moraes de Castro Souza ◽  
Bruno Henrique Saranholi ◽  
Peter Gransden Crawshaw Jr. ◽  
Agustin Javier Paviolo ◽  
Lilian Elaine Rampim ◽  
...  
Keyword(s):  
Atlantic Forest ◽  
Protected Area ◽  
National Park ◽  
Dna Analysis ◽  
Southeastern Brazil ◽  
Top Predator ◽  
Resident Population ◽  
Serra Do Mar ◽  
Non Invasive ◽  
The Status

Abstract Jaguar populations have been declining in Brazil mostly due to habitat loss and fragmentation, conflict with humans, poaching and reduction of prey. This is dramatically true in the Atlantic Forest, where occurrence of this large felid is now restricted to very few remaining areas. We used a non-invasive DNA analysis to search through felid scats collected in the Santa Virginia Unit (SVU) of the Serra do Mar State Park, to test for the potential presence of jaguar there. Our results indicated at least three individuals (two females and one male) inside SVU, thus confirming at least temporary presence of this top predator in this important protected area. It is now crucial to intensify studies in that area and surroundings, to evaluate the status of these individuals and identify conservation needs to urgently improve the prospects for the establishment of a resident population, allowing it to expand to adjoining units of the Serra do Mar State Park and Serra da Bocaina National Park.

scholarly journals Optimal non‐invasive diagnosis of fetal achondroplasia combining ultrasonography with circulating cell‐free fetal DNA analysis

2018 ◽  
Vol 53 (1) ◽  
pp. 87-94 ◽  
Author(s):  
A. J. Vivanti ◽  
J.‐M. Costa ◽  
A. Rosefort ◽  
P. Kleinfinger ◽  
L. Lohmann ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Non Invasive ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals Environmental DNA as a non‐invasive sampling tool to detect the spawning distribution of European anadromous shads ( Alosa  spp.)

2019 ◽  
Vol 29 (1) ◽  
pp. 148-152 ◽  
Author(s):  
Caterina Maria Antognazza ◽  
J. Robert Britton ◽  
Caitlin Potter ◽  
Elizabeth Franklin ◽  
Emilie A. Hardouin ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Non Invasive

A next generation sequencing approach for targeted Varroa destructor (Acari: Varroidae) mitochondrial DNA analysis based on honey derived environmental DNA

2019 ◽  
Vol 161 ◽  
pp. 47-53 ◽  
Author(s):  
Valerio Joe Utzeri ◽  
Giuseppina Schiavo ◽  
Anisa Ribani ◽  
Francesca Bertolini ◽  
Samuele Bovo ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Next Generation Sequencing ◽  
Varroa Destructor ◽  
Dna Analysis ◽  
Environmental Dna ◽  
Next Generation ◽  
Mitochondrial Dna Analysis ◽  
Generation Sequencing

scholarly journals Two Reliable Methodical Approaches for Non-Invasive RHD Genotyping of a Fetus from Maternal Plasma

Diagnostics ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 564
Author(s):  
Jana Bohmova ◽  
Marek Lubusky ◽  
Iva Holuskova ◽  
Martina Studnickova ◽  
Romana Kratochvilova ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Analysis ◽  
Methodological Approach ◽  
Maternal Plasma ◽  
Buccal Swab ◽  
Non Invasive ◽  
Pcr Method ◽  
Parallel Reactions

Noninvasive fetal RHD genotyping is an important tool for predicting RhD incompatibility between a pregnant woman and a fetus. This study aimed to assess a methodological approach other than the commonly used one for noninvasive fetal RHD genotyping on a representative set of RhD-negative pregnant women. The methodology must be accurate, reliable, and broadly available for implementation into routine clinical practice. A total of 337 RhD-negative pregnant women from the Czech Republic region were tested in this study. The fetal RHD genotype was assessed using two methods: real-time PCR and endpoint quantitative fluorescent (QF) PCR. We used exon-7-specific primers from the RHD gene, along with internal controls. Plasma samples were analyzed and measured in four/two parallel reactions to determine the accuracy of the RHD genotyping. The RHD genotype was verified using DNA analysis from a newborn buccal swab. Both methods showed an excellent ability to predict the RHD genotype. Real-time PCR achieved its greatest accuracy of 98.6% (97.1% sensitivity and 100% specificity (95% CI)) if all four PCRs were positive/negative. The QF PCR method also achieved its greatest accuracy of 99.4% (100% sensitivity and 98.6% specificity (95% CI)) if all the measurements were positive/negative. Both real-time PCR and QF PCR were reliable methods for precisely assessing the fetal RHD allele from the plasma of RhD-negative pregnant women.

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