Macroinvertebrate and diatom indicators of baseline conditions for setting targets in agricultural BMP restoration

Background:The Observational and Medical Outcomes Partnerships (OMOP) common data model (CDM) provides a framework for standardising health data.Objectives:To map national biologic registry data collected from different European countries to the OMOP CDM.Methods:Five biologic registries are currently being mapped to the OMOP CDM: 1) the Czech biologics register (ATTRA), 2) Registro Español de Acontecimientos Adversos de Terapias Biológicas en Enfermedades Reumáticas (BIOBADASER), 3) British Society for Rheumatology Biologics Register for Rheumatoid Arthritis (BSRBR-RA), 4) German biologics register ‘Rheumatoid arthritis observation of biologic therapy’ (RABBIT), and 5) Swiss register ’Swiss Clinical Quality Management in Rheumatic Diseases’ (SCQM).Data collected at baseline are being mapped first. Details that uniquely identify individuals are mapped to the person table, with the observation_period table defining the time a person may have had clinical events recorded. Baseline comorbidities are mapped to the condition_occurrence CDM table, while baseline medications are mapped to the drug_exposure CDM table. This mapping is summarised in Figure 1.Figure 1.Overview of initial mappingResults:A total of 64,901 individuals are included in the 5 registries being mapped to the OMOP CDM, see table 1. The number of unique baseline conditions being mapped range from 17 in BSRBR-RA to 108 in RABBIT, while the number of baseline medications range from 26 in ATTRA to 802 in BSRBR-RA. Those registries which captured more comorbidities or medications generally allowed for these to be inputted as free text.Table 1.Summary of initial code mappingRegistryNumber of individualsNumber of mapped baseline conditionsNumber of mapped baseline medicationsATTRA5,3262626BIOBADASER6,4963051BSRBR-RA21,69517802RABBIT13,06210878SCQM18,3222633Conclusion:Due to differences in study design and data capture, the baseline information captured on comorbidities and drugs across registries varies greatly. However, these data have been mapped and mapping biologic registry data to the OMOP CDM is feasible. The adoption of the OMOP CDM will facilitate collaboration across registries and allow for multi-database studies which include data from both biologic registries and other sources of health data which have been mapped to the CDM.Disclosure of Interests:Edward Burn: None declared, Lianne Kearsley-Fleet: None declared, Kimme Hyrich Grant/research support from: Pfizer, UCB, BMS, Speakers bureau: Abbvie, Martin Schaefer: None declared, Doreen Huschek: None declared, Anja Strangfeld Speakers bureau: AbbVie, BMS, Pfizer, Roche, Sanofi-Aventis, Jakub Zavada Speakers bureau: Abbvie, UCB, Sanofi, Elli-Lilly, Novartis, Zentiva, Accord, Markéta Lagová: None declared, Delphine Courvoisier: None declared, Christoph Tellenbach: None declared, Kim Lauper: None declared, Carlos Sánchez-Piedra: None declared, Nuria Montero: None declared, Jesús-Tomás Sanchez-Costa: None declared, Daniel Prieto-Alhambra Grant/research support from: Professor Prieto-Alhambra has received research Grants from AMGEN, UCB Biopharma and Les Laboratoires Servier, Consultant of: DPA’s department has received fees for consultancy services from UCB Biopharma, Speakers bureau: DPA’s department has received fees for speaker and advisory board membership services from Amgen

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Altered V-ATPase expression in renal intercalated cells isolated from B1 subunit-deficient mice by fluorescence-activated cell sorting

AJP Renal Physiology ◽

10.1152/ajprenal.00394.2012 ◽

2013 ◽

Vol 304 (5) ◽

pp. F522-F532 ◽

Cited By ~ 21

Author(s):

Luca Vedovelli ◽

John T. Rothermel ◽

Karin E. Finberg ◽

Carsten A. Wagner ◽

Anie Azroyan ◽

...

Keyword(s):

Cell Sorting ◽

Collecting Duct ◽

Egfp Expression ◽

Intercalated Cells ◽

Wild Type ◽

Western Blots ◽

Atpase Subunit ◽

Protein Levels ◽

Baseline Conditions ◽

Deficient Mice

Unlike human patients with mutations in the 56-kDa B1 subunit isoform of the vacuolar proton-pumping ATPase (V-ATPase), B1-deficient mice (Atp6v1b1−/−) do not develop metabolic acidosis under baseline conditions. This is due to the insertion of V-ATPases containing the alternative B2 subunit isoform into the apical membrane of renal medullary collecting duct intercalated cells (ICs). We previously reported that quantitative Western blots (WBs) from whole kidneys showed similar B2 protein levels in Atp6v1b1−/− and wild-type mice (Păunescu TG, Russo LM, Da Silva N, Kovacikova J, Mohebbi N, Van Hoek AN, McKee M, Wagner CA, Breton S, Brown D. Am J Physiol Renal Physiol 293: F1915–F1926, 2007). However, WBs from renal medulla (including outer and inner medulla) membrane and cytosol fractions reveal a decrease in the levels of the ubiquitous V-ATPase E1 subunit. To compare V-ATPase expression specifically in ICs from wild-type and Atp6v1b1−/− mice, we crossed mice in which EGFP expression is driven by the B1 subunit promoter (EGFP-B1+/+ mice) with Atp6v1b1−/− mice to generate novel EGFP-B1−/− mice. We isolated pure IC populations by fluorescence-assisted cell sorting from EGFP-B1+/+ and EGFP-B1−/− mice to compare their V-ATPase subunit protein levels. We report that V-ATPase A, E1, and H subunits are all significantly downregulated in EGFP-B1−/− mice, while the B2 protein level is considerably increased in these animals. We conclude that under baseline conditions B2 upregulation compensates for the lack of B1 and is sufficient to maintain basal acid-base homeostasis, even when other V-ATPase subunits are downregulated.

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Abstract 403: Renal Denervation Reveals Renal Sympathetic Activation in Adult Rats Exposed to Early Life Stress

Hypertension ◽

10.1161/hyp.60.suppl_1.a403 ◽

2012 ◽

Vol 60 (suppl_1) ◽

Author(s):

Analia S Loria ◽

Michael W Brands ◽

David M Pollock ◽

Jennifer S Pollock

Keyword(s):

Life Stress ◽

Early Life ◽

Renal Denervation ◽

Early Life Stress ◽

Male Rats ◽

Renal Nerves ◽

Adult Rats ◽

Baseline Conditions ◽

Control Sham ◽

Renal Sympathetic

We previously reported that maternal separation (MS), a model of early life stress, does not modify baseline blood pressure in adult rats, but increases sensitivity to hypertensive stimuli. Under baseline conditions, adult male rats exposed to MS have significantly reduced glomerular filtration rate (GFR). Acute phenylephrine-induced reductions in renal blood flow is significantly attenuated in rats exposed to MS compared to control rats. Furthermore, norephinephrine (NE) content was increased in renal cortex of MS rats compared to control rats (p<0.05). These data indicate that MS induces increased renal sympathetic outflow. Thus, we hypothesized that renal denervation will normalize GFR in rats exposed to MS. Male WKY rat pups were separated from their mothers for 3 hrs/day during the morning hours from day 2 to 14 of life. Male non-separated littermates served as control rats. Experiments were performed in 300-320 g adult rats. Denervation (DnX) was performed mechanically stripping all visible renal nerves followed by topical phenol (10%) on the renal artery. Control-sham, MS-sham, control-DnX, and MS-DnX rats were instrumented with catheters in the femoral vein and abdominal aorta. Rats were placed in metabolic cages, connected to swivels, and allowed to recover for 4-5 days. Sodium intake was clamped at 2.8 mEq/day in both groups by combining sodium deficient diet and 24 hr/day 0.9% iv saline infusion (20 ml/day). GFR was determined by plasma clearance of [125I]iothalamate in the conscious state. During baseline conditions, MAP was not different between control-sham and MS-sham rats (99±4 vs 97±2 mmHg, respectively). MAP was reduced in both control-DnX and MS-DnX rats (91±2 mmHg and 83±3 mmHg, p<0.05, respectively) compared with the respective sham group. The reduction in MAP tended to be greater in MS than in control rats (-9±1 and -14±2 mmHg, p=0.074). DnX did not modify GFR in control rats (sham: 3.1±0.1 ml/min vs DnX: 3.5±0.4 ml/min). However, DnX significantly increased GFR in rats exposed to MS (sham: 2.4±0.2 ml/min vs DnX: 3.8±0.4 ml/min, p<0.05). These data support our hypothesis that MS induces increased renal sympathetic tone to reduce GFR in MS male rats, and may contribute to the exacerbated response to hypertensive stimuli observed in MS rats.

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Scaling vs. nonscaling methods of assessing autonomic tone in streptozotocin-induced diabetic rats

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00519.2001 ◽

2002 ◽

Vol 283 (3) ◽

pp. H1142-H1149 ◽

Cited By ~ 4

Author(s):

Itay Perlstein ◽

Nir Sapir ◽

Joshua Backon ◽

Dan Sapoznikov ◽

Roman Karasik ◽

...

Keyword(s):

Cross Correlation ◽

Diabetic Rats ◽

Fluctuation Analysis ◽

Autoregressive Modeling ◽

Autonomic Tone ◽

Scaling Parameter ◽

Average Value ◽

Disease States ◽

Streptozotocin Induced Diabetes ◽

Baseline Conditions

We studied heart rate variability in rats by power scaling spectral analysis (PSSA), autoregressive modeling (AR), and detrended fluctuation analysis (DFA), assessed stability by coefficient of variation between consecutive 6-h epochs, and then compared cross-correlation among techniques. These same parameters were checked from baseline conditions through acute and chronic disease states (streptozotocin-induced diabetes) followed by therapeutic intervention (insulin). Cross-correlation between methods over the entire time period was r = 0.94 (DFA and PSSA), r = 0.81 (DFA and AR), and r = 0.77 (AR and PSSA). Under baseline conditions the scaling parameter measured by DFA and PSSA and the high-frequency (HF) component measured by AR fluctuated around an average value, but these fluctuations were different for the three methods. After diabetes induction, a strong correlation was found between the HF power and the short-term scaling parameter. Despite their differences in methodology, DFA and PSSA assess changes in parasympathetic tone as detected by autoregressive modeling.

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Self-Management of Schedule following in Three Teenagers with Autism

Behavioral Disorders ◽

10.1177/019874299502000304 ◽

1995 ◽

Vol 20 (3) ◽

pp. 190-196 ◽

Cited By ~ 29

Author(s):

Bobby Newman ◽

Dawn M. Buffington ◽

Mairead A. O'grady ◽

Mary E. Mcdonald ◽

Claire L. Poulson ◽

...

Keyword(s):

Treatment Phase ◽

Noncontingent Reinforcement ◽

Transition Time ◽

Multiple Baseline ◽

Self Management ◽

Accurate Identification ◽

Baseline Conditions ◽

Transition Times

A multiple baseline across students design was used to investigate the effects of a self-management package on schedule following by three teenagers with autism. During baseline conditions, noncontingent reinforcement was provided. In the treatment phase, students contingently self-reinforced the verbal identification of transition times. Systematic increases in accurate identification of transitions were observed across all students. Accurate identification of transition time and self-reinforcement were maintained in a one-month follow-up.

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The role of NOS2 and NOS3 in renal protein and arginine metabolism during early endotoxemia in mice

AJP Renal Physiology ◽

10.1152/ajprenal.00308.2004 ◽

2005 ◽

Vol 288 (4) ◽

pp. F816-F822 ◽

Cited By ~ 6

Author(s):

Yvette C. Luiking ◽

Marcella M. Hallemeesch ◽

Wouter H. Lamers ◽

Nicolaas E. P. Deutz

Keyword(s):

Blood Flow ◽

Renal Blood Flow ◽

Protein Turnover ◽

De Novo ◽

Whole Body ◽

Wild Type ◽

Protein Amino Acid ◽

Renal Metabolism ◽

Lps Challenge ◽

Baseline Conditions

Previously, we observed an enhanced renal protein synthesis and increased de novo arginine production in the early response to endotoxemia in wild-type Swiss mice (Hallemeesch MM, Soeters PB, and Deutz NE. Am J Physiol Renal Physiol 282: F316–F323, 2002). To establish whether these changes are regulated by nitric oxide (NO) synthesized by NO synthase isoforms NOS2 and NOS3, we studied C57BL6/J wild-type (WT), NOS2-deficient (NOS2−/−), and NOS3-deficient (NOS3−/−) mice under baseline (unstimulated) and LPS-treated conditions. The metabolism of renal protein, amino acid, and arginine was studied at the whole body level and across the kidney by infusing the stable isotopes l-[phenyl-2H5]phenylalanine, l-[phenyl-2H2]tyrosine, l- guanidino-[15N2]arginine, and l-[ ureido-13C,2H2]citrulline. Renal blood flow was measured using radioactive PAH extraction. Under baseline conditions, renal blood flow was significantly reduced in NOS2−/− mice (0.29 ± 0.01 vs. 0.48 ± 0.07 ml·10 g body wt−1·min−1 in WT) ( P < 0.05), and de novo arginine production was lower in NOS2−/− mice. After LPS challenge, renal protein turnover and arginine production increased in all three groups ( P < 0.05), even though renal de novo arginine synthesis did not increase. The expected increase in renal citrulline production and disposal after LPS was not observed in NOS2−/− mice ( P = 0.06). Collectively, these data show that NOS2 is constitutively expressed in the kidney and remarkably functional as it affects renal blood flow and de novo arginine production under baseline conditions and is important for the increase in renal citrulline turnover during endotoxemia. NOS3, in contrast, appears less important for renal metabolism. The increase in renal protein turnover during endotoxemia does not depend on NOS2 or NOS3 activity.

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Abstract 772: The Highly Prevalent 25bp Intronic Deletion in MYBPC3 is Benign Under Baseline Conditions

Circulation Research ◽

10.1161/res.125.suppl_1.772 ◽

2019 ◽

Vol 125 (Suppl_1) ◽

Author(s):

James W McNamara ◽

Jennifer A Schwanekamp ◽

Parth N Patel ◽

Shiv K Viswanathan ◽

Mohammad Bohlooly ◽

...

Keyword(s):

Baseline Conditions

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Transfer function analysis between arterial pressure and renal sympathetic nerve activity at cardiac pacing frequencies in the rat

Journal of Applied Physiology ◽

10.1152/japplphysiol.01064.2006 ◽

2007 ◽

Vol 102 (3) ◽

pp. 1034-1040 ◽

Cited By ~ 5

Author(s):

Valérie Oréa ◽

Roy Kanbar ◽

Bruno Chapuis ◽

Christian Barrès ◽

Claude Julien

Keyword(s):

Transfer Function ◽

Arterial Pressure ◽

Sympathetic Nerve ◽

Sympathetic Nerve Activity ◽

Cardiac Pacing ◽

Nerve Activity ◽

Renal Sympathetic Nerve Activity ◽

Renal Sympathetic Nerve ◽

Baseline Conditions ◽

Renal Sympathetic

This study examined the possible influence of changes in heart rate (HR) on the gain of the transfer function relating renal sympathetic nerve activity (RSNA) to arterial pressure (AP) at HR frequency in rats. In seven urethane-anesthetized rats, AP and RSNA were recorded under baseline conditions (spontaneous HR = 338 ± 6 beats/min, i.e., 5.6 ± 0.1 Hz) and during 70-s periods of cardiac pacing at 6–9 Hz applied in random order. Cardiac pacing slightly increased mean AP (0.8 ± 0.2 mmHg/Hz) and decreased pulse pressure (−3.6 ± 0.3 mmHg/Hz) while leaving the mean level of RSNA essentially unaltered ( P = 0.680, repeated-measures ANOVA). The gain of the transfer function from AP to RSNA measured at HR frequency was always associated with a strong, significant coherence and was stable between 6 and 9 Hz ( P = 0.185). The transfer function gain measured under baseline conditions [2.44 ± 0.28 normalized units (NU)/mmHg] did not differ from that measured during cardiac pacing (2.46 ± 0.27 NU/mmHg). On the contrary, phase decreased linearly as a function of HR, which indicated the presence of a fixed time delay (97 ± 6 ms) between AP and RSNA. In conclusion, the dynamic properties of arterial baroreflex pathways do not affect the gain of the transfer function between AP and RSNA measured at HR frequency in the upper part of the physiological range of HR variations in the rat.

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