scholarly journals A round‐robin evaluation of the repeatability and reproducibility of environmental DNA assays for dreissenid mussels

2020 ◽  
Vol 2 (4) ◽  
pp. 446-459 ◽  
Author(s):  
Adam J. Sepulveda ◽  
Patrick R. Hutchins ◽  
Craig Jackson ◽  
Carl Ostberg ◽  
Matthew B. Laramie ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Round Robin ◽  
Dreissenid Mussels ◽  
Dna Assays ◽  
Repeatability And Reproducibility

scholarly journals A validation scale to determine the readiness of environmental DNA assays for routine species monitoring

2021 ◽  
Author(s):  
Bettina Thalinger ◽  
Kristy Deiner ◽  
Lynsey R. Harper ◽  
Helen C. Rees ◽  
Rosetta C. Blackman ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Dna Assays

scholarly journals Environmental DNA assays for the sister taxa sauger (Sander canadensis) and walleye (Sander vitreus)

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0176459 ◽  
Author(s):  
Joseph C. Dysthe ◽  
Kellie J. Carim ◽  
Michael Ruggles ◽  
Kevin S. McKelvey ◽  
Michael K. Young ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Sander Vitreus ◽  
Dna Assays

scholarly journals Reporting the limits of detection and quantification for environmental DNA assays

2019 ◽  
Vol 2 (3) ◽  
pp. 271-282 ◽  
Author(s):  
Katy E. Klymus ◽  
Christopher M. Merkes ◽  
Michael J. Allison ◽  
Caren S. Goldberg ◽  
Caren C. Helbing ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Limits Of Detection ◽  
Dna Assays ◽  
Detection And Quantification

scholarly journals Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Luis Peñarrubia ◽  
Carles Alcaraz ◽  
Abraham bij de Vaate ◽  
Nuria Sanz ◽  
Carles Pla ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Dreissenid Mussels

scholarly journals Correction: Schistosoma species detection by environmental DNA assays in African freshwaters

2020 ◽  
Vol 14 (9) ◽  
pp. e0008721
Author(s):  
Hind Alzaylaee ◽  
Rupert A. Collins ◽  
Gabriel Rinaldi ◽  
Asilatu Shechonge ◽  
Benjamin Ngatunga ◽  
...  
Keyword(s):  
Environmental Dna ◽  
Species Detection ◽  
Dna Assays

scholarly journals GoFish: A versatile nested PCR strategy for environmental DNA assays for marine vertebrates

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0198717 ◽  
Author(s):  
Mark Y. Stoeckle ◽  
Mithun Das Mishu ◽  
Zachary Charlop-Powers
Keyword(s):  
Nested Pcr ◽  
Environmental Dna ◽  
Dna Assays ◽  
Marine Vertebrates

scholarly journals Using environmental DNA to extend the window of early detection for dreissenid mussels

2019 ◽  
Vol 10 (2) ◽  
pp. 342-358 ◽  
Author(s):  
Adam Sepulveda
Keyword(s):  
Early Detection ◽  
Environmental Dna ◽  
Dreissenid Mussels

Round-Robin Testing of a New EPA Solidifier Effectiveness Protocol

2017 ◽  
Vol 2017 (1) ◽  
pp. 1737-1748
Author(s):  
Devi Sundaravadivelu ◽  
Robyn N. Conmy ◽  
Pablo Campo
Keyword(s):  
Environmental Protection Agency ◽  
Control Sample ◽  
Standard Operating Procedure ◽  
Round Robin ◽  
Round Robin Test ◽  
Operator Error ◽  
Testing Protocol ◽  
Repeatability And Reproducibility ◽  
Product Effectiveness ◽  
Operational Errors

ABSTRACT A new laboratory testing protocol for evaluating effectiveness of solidifiers in removing crude oil slicks on the water surface has been developed by the U.S. Environmental Protection Agency (EPA). Since an assessment of a testing protocol requires validation through an inter laboratory study with several independent research partners, a round-robin test was performed by five operators across two laboratories. The protocol was qualitatively and quantitatively evaluated to determine if it can satisfactorily differentiate product effectiveness, while still accounting for experimental and operational errors. Each operator evaluated the efficiency of six solidifier products with Arabian Light, Endicott and IFO 120 crude oils. All the experiments were carried out in triplicates and, additionally, an oil alone control sample was run for quality control purposes. The five operators were provided with all the supplies required to conduct the experiment and a detailed standard operating procedure. The results were collected and analyzed statistically to quantify repeatability and reproducibility. The average repeatability and reproducibility standard deviations were 5.1, 2.7, and 3.3% and 11.2, 10.1, and 13.1% for Arabian Light, Endicott and IFO, respectively. The within-operator error was ≤ 5% regardless of operator and oil type. Due to the difficulty and uncertainty associated with the separation of the solidified and un-solidified mass, the between-operator error was around 10%. The oil alone control had an average repeatability and reproducibility standard deviation of 4.8 and 6.1%, respectively. The variability among operators was also examined with h-statistics and the differences in operator means were found to be statistically insignificant. The variability is deemed acceptable for purposes of differentiating effective from ineffective solidifier products in the laboratory. The results from this round robin experiment will serve as an initial screening tool for the solidifier products in the market and will also help response teams determine the desirability and appropriateness of using a specific solidifier for oil spill remediation.

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