Real Time In Vivo Measurement of Ascorbate in the Brain Using Carbon Nanotube-Modified Microelectrodes

2013 ◽  
Vol 25 (7) ◽  
pp. 1757-1763 ◽  
Author(s):  
Nuno R. Ferreira ◽  
Ricardo M. Santos ◽  
João Laranjinha ◽  
Rui M. Barbosa
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
In Vivo Measurement ◽  
The Brain

scholarly journals Real time, in vivo measurement of neuronal and peripheral clocks in Drosophila melanogaster

2022 ◽  
Author(s):  
Peter S Johnstone ◽  
Maite Ogueta ◽  
Inan Top ◽  
Sheyum Syed ◽  
Ralf Stanewsky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Circadian Clocks ◽  
Specific Gene ◽  
Tissue Specific ◽  
In Vivo Measurement ◽  
Neuronal Tissues ◽  
Peripheral Clocks ◽  
The Brain

Circadian clocks are highly conserved transcriptional regulators that control 24-hour oscillations in gene expression, physiological function, and behavior. Circadian clocks exist in almost every tissue and are thought to control tissue-specific gene expression and function, synchronized by the brain clock. Many disease states are associated with loss of circadian regulation. How and when circadian clocks fail during pathogenesis remains largely unknown because it is currently difficult to monitor tissue-specific clock function in intact organisms. Here, we developed a method to directly measure the transcriptional oscillation of distinct neuronal and peripheral clocks in live, intact Drosophila, which we term Locally Activatable BioLuminescence or LABL. Using this method, we observed that specific neuronal and peripheral clocks exhibit distinct transcription properties. Loss of the receptor for PDF, a circadian neurotransmitter critical for the function of the brain clock, disrupts circadian locomotor activity but not all tissue-specific circadian clocks; we found that, while peripheral clocks in non-neuronal tissues were less stable after the loss of PDF signaling, they continued to oscillate. This result suggests that the presumed dominance of the brain clock in regulating peripheral clocks needs to be re-examined. This result further demonstrates that LABL allows rapid, affordable, and direct real-time monitoring of clocks in vivo.

Voltammetric Detection of Tetrodotoxin Real-Time In Vivo of Mouse Organs using DNA-Immobilized Carbon Nanotube Sensors

2019 ◽  
Vol 15 (5) ◽  
pp. 567-574
Author(s):  
Huck Jun Hong ◽  
Suw Young Ly
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Anticancer Agents ◽  
Cancer Metastasis ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Takifugu Rubripes ◽  
In Vivo Detection ◽  
Voltammetric Detection

Background: Tetrodotoxin (TTX) is a biosynthesized neurotoxin that exhibits powerful anticancer and analgesic abilities by inhibiting voltage-gated sodium channels that are crucial for cancer metastasis and pain delivery. However, for the toxin’s future medical applications to come true, accurate, inexpensive, and real-time in vivo detection of TTX remains as a fundamental step. Methods: In this study, highly purified TTX extracted from organs of Takifugu rubripes was injected and detected in vivo of mouse organs (liver, heart, and intestines) using Cyclic Voltammetry (CV) and Square Wave Anodic Stripping Voltammetry (SWASV) for the first time. In vivo detection of TTX was performed with auxiliary, reference, and working herring sperm DNA-immobilized carbon nanotube sensor systems. Results: DNA-immobilization and optimization of amplitude (V), stripping time (sec), increment (mV), and frequency (Hz) parameters for utilized sensors amplified detected peak currents, while highly sensitive in vivo detection limits, 3.43 µg L-1 for CV and 1.21 µg L-1 for SWASV, were attained. Developed sensors herein were confirmed to be more sensitive and selective than conventional graphite rodelectrodes modified likewise. A linear relationship was observed between injected TTX concentration and anodic spike peak height. Microscopic examination displayed coagulation and abnormalities in mouse organs, confirming the powerful neurotoxicity of extracted TTX. Conclusion: These results established the diagnostic measures for TTX detection regarding in vivo application of neurotoxin-deviated anticancer agents and analgesics, as well as TTX from food poisoning and environmental contamination.

scholarly journals Live View of Gonadotropin-Releasing Hormone Containing Neuron Migration

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 463-468 ◽  
Author(s):  
Elizabeth P. Bless ◽  
Heather J. Walker ◽  
Kwok W. Yu ◽  
J. Gabriel Knoll ◽  
Suzanne M. Moenter ◽  
...  
Keyword(s):  
Real Time ◽  
Direct Evidence ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Reproductive Axis ◽  
Gnrh Neurons ◽  
Migratory Route ◽  
The Brain

Neurons that synthesize GnRH control the reproductive axis and migrate over long distances and through different environments during development. Prior studies provided strong clues for the types of molecules encountered and movements expected along the migratory route. However, our studies provide the first real-time views of the behavior of GnRH neurons in the context of an in vitro preparation that maintains conditions comparable to those in vivo. The live views provide direct evidence of the changing behavior of GnRH neurons in their different environments, showing that GnRH neurons move with greater frequency and with more changes in direction after they enter the brain. Perturbations of guiding fibers distal to moving GnRH neurons in the nasal compartment influenced movement without detectable changes in the fibers in the immediate vicinity of moving GnRH neurons. This suggests that the use of fibers by GnRH neurons for guidance may entail selective signaling in addition to mechanical guidance. These studies establish a model to evaluate the influences of specific molecules that are important for their migration.

Real-Time Quantitation of Renal Ischemia Using Targeted Microbubbles: In-vivo Measurement of P-selectin Expression

2009 ◽  
Vol 23 (3) ◽  
pp. 373-378 ◽  
Author(s):  
Sero Andonian ◽  
Tonya Coulthard ◽  
Arthur D. Smith ◽  
Pravin S. Singhal ◽  
Benjamin R. Lee
Keyword(s):  
Real Time ◽  
Renal Ischemia ◽  
In Vivo Measurement ◽  
Targeted Microbubbles

scholarly journals The brain interstitial system: Anatomy, modeling, in vivo measurement, and applications

2017 ◽  
Vol 157 ◽  
pp. 230-246 ◽  
Author(s):  
Yiming Lei ◽  
Hongbin Han ◽  
Fan Yuan ◽  
Aqeel Javeed ◽  
Yong Zhao
Keyword(s):  
In Vivo Measurement ◽  
The Brain
Sign in / Sign up

Export Citation Format

Share Document