Electrochemical Aptasensor Based on PEI‐rGO/AuNWs and Zr‐MOF for Determination of Adenosine Triphosphate via Exonuclease Ⅰ‐assisted Target Recycling Strategy

2021 ◽  
Author(s):  
Ruina Zheng ◽  
Baoshan He ◽  
lingling xie ◽  
li liping ◽  
yang jinping ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Electrochemical Aptasensor ◽  
Target Recycling

Electrochemical aptasensor based on Mo2C/Mo2N and gold nanoparticles for determination of chlorpyrifos

2021 ◽  
Vol 188 (5) ◽  
Author(s):  
Zhenfeng Lin ◽  
Xin Liu ◽  
Yangzi Li ◽  
Changxiang Li ◽  
Liu Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Aptasensor

scholarly journals Immobilization-Free Electrochemical Sensor Coupled with a Graphene-Oxide-Based Aptasensor for Glycated Albumin Detection

Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 85
Author(s):  
Wassa Waiwinya ◽  
Thitirat Putnin ◽  
Dechnarong Pimalai ◽  
Wireeya Chawjiraphan ◽  
Nuankanya Sathirapongsasuti ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Electrochemical Sensor ◽  
Detection Method ◽  
Limit Of Detection ◽  
Glycated Albumin ◽  
Cost Effective ◽  
Logarithmic Scale ◽  
Electrochemical Aptasensor ◽  
Alternative Approach

An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin; their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01–50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels.

scholarly journals Adenosine Triphosphate Measurement in Deep Sea Using a Microfluidic Device

Micromachines ◽  
2018 ◽  
Vol 9 (8) ◽  
pp. 370 ◽  
Author(s):  
Tatsuhiro Fukuba ◽  
Takuroh Noguchi ◽  
Kei Okamura ◽  
Teruo Fujii
Keyword(s):  
Adenosine Triphosphate ◽  
Microfluidic Device ◽  
Deep Sea ◽  
Temperature Sensor ◽  
Biochemical Parameter ◽  
Underwater Vehicle ◽  
Luciferase Assay ◽  
Bioluminescence Intensity

Total ATP (adenosine triphosphate) concentration is a useful biochemical parameter for detecting microbial biomass or biogeochemical activity anomalies in the natural environment. In this study, we describe the development and evaluation of a new version of in situ ATP analyzer improved for the continuous and quantitative determination of ATP in submarine environments. We integrated a transparent microfluidic device containing a microchannel for cell lysis and a channel for the bioluminescence L–L (luciferin–luciferase) assay with a miniature pumping unit and a photometry module for the measurement of the bioluminescence intensity. A heater and a temperature sensor were also included in the system to maintain an optimal temperature for the L–L reaction. In this study, the analyzer was evaluated in deep sea environments, reaching a depth of 200 m using a remotely operated underwater vehicle. We show that the ATP analyzer successfully operated in the deep-sea environment and accurately quantified total ATP within the concentration lower than 5 × 10−11 M.

Determination of Adenosine Triphosphate by a Target Inhibited Catalytic Cycle Based on a Strand Displacement Reaction

2014 ◽  
Vol 47 (3) ◽  
pp. 478-491 ◽  
Author(s):  
Sheng Cheng ◽  
Bin Zheng ◽  
Mozhen Wang ◽  
Qing Zhao ◽  
Michael Hon-Wah Lam ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Catalytic Cycle ◽  
Displacement Reaction ◽  
Strand Displacement ◽  
Strand Displacement Reaction
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