A high resolution two-dimensional gel electrophoresis and silver staining protocol demonstrated with nuclear matrix proteins

1990 ◽  
Vol 11 (6) ◽  
pp. 500-504 ◽  
Author(s):  
James F. Cupo ◽  
Graham P. Lidgard ◽  
William F. Lichtman
Keyword(s):  
High Resolution ◽  
Nuclear Matrix ◽  
Silver Staining ◽  
Gel Electrophoresis ◽  
Matrix Proteins ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Nuclear Matrix Proteins ◽  
Staining Protocol

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

scholarly journals Heterogeneity of human haptoglobin α chains detected by two-dimensional gel electrophoresis

1987 ◽  
Vol 50 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Huw A. John ◽  
Ian F. Purdom
Keyword(s):  
Human Plasma ◽  
Silver Staining ◽  
Gel Electrophoresis ◽  
Plasma Samples ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Human Plasma Samples ◽  
Low Levels

SummaryThe protein spots representing the haptoglobin α1F, α1Sand α2chains in two-dimensional gels of human plasma samples representative of the six common haptoglobin phenotypes were identified by comparing their position with those of purified haptoglobin and distinguished from other spots in the vicinity by comparison with plasma with undetectably low levels of haptoglobin. Silver staining indicated that the α1Fchain was represented by one spot in subtypes 1F-1F, 1F-1S and 1F-2, the α1Schain by three spots in subtypes 1S-1S, 1F-1S and 1S-2 and the α2chain by six spots in 1F-2, 1S-2 and 2–2

Technology: Methodology for High-Resolution Two-Dimensional Gel Electrophoresis

1987 ◽  
Vol 5 (2) ◽  
pp. 127-149 ◽  
Author(s):  
A Thomas Endler ◽  
Donald S. Young ◽  
Russell P. Tracy
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis
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