Discriminative detection of low-abundance point mutations using a PCR/ligase detection reaction/capillary gel electrophoresis method and fluorescence dual-channel monitoring

2014 ◽  
Vol 35 (8) ◽  
pp. 1204-1210 ◽  
Author(s):  
Mariko Hamada ◽  
Koji Shimase ◽  
Kazuhiko Tsukagoshi ◽  
Masahiko Hashimoto
Keyword(s):  
Gel Electrophoresis ◽  
Point Mutations ◽  
Ligase Detection Reaction ◽  
Capillary Gel Electrophoresis ◽  
Dual Channel ◽  
Electrophoresis Method

Development of a capillary gel electrophoresis method for monitoring disulfide isomer heterogeneity in IgG2 antibodies

2010 ◽  
Vol 31 (3) ◽  
pp. 448-458 ◽  
Author(s):  
Nathan A. Lacher ◽  
Qian Wang ◽  
Rachel K. Roberts ◽  
Heidi J. Holovics ◽  
Serdar Aykent ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Capillary Gel Electrophoresis ◽  
Disulfide Isomer ◽  
Electrophoresis Method

Development of a chip-based capillary gel electrophoresis method for quantification of a half-antibody in immunoglobulin G4 samples

2004 ◽  
Vol 25 (21-22) ◽  
pp. 3890-3896 ◽  
Author(s):  
Elena Vasilyeva ◽  
James Woodard ◽  
Frederick R. Taylor ◽  
Matthias Kretschmer ◽  
Hans Fajardo ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Immunoglobulin G4 ◽  
Capillary Gel Electrophoresis ◽  
Electrophoresis Method

Establishment of a Decaplex PCR-Capillary Gel Electrophoresis Method for the Simultaneous Detection of Six Kinds of Genetically Modified Animals

2018 ◽  
Vol 101 (2) ◽  
pp. 601-606 ◽  
Author(s):  
Xiaofei Liu ◽  
Songyin Qiu ◽  
Xiaolin Li ◽  
Dandan Liu ◽  
Hongli Jing ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Gel Electrophoresis ◽  
Detection System ◽  
Genetically Modified ◽  
Simultaneous Detection ◽  
Fatty Acid Desaturase ◽  
Capillary Gel Electrophoresis ◽  
Inspection And Quarantine ◽  
Electrophoresis Method ◽  
Gm Animals

Abstract This study aimed to establish an event-specific multiplex PCR system using microsatellite markers and fluorescently labeled primers to detect six different genetically modified (GM) animal lines, including human lactoferrin GM cattle, human lysozyme GM cattle, human α-lactalbumin GM cattle, myostatin knockout pigs, phytase GM pigs, and ω-3 fatty acid desaturase gene GM pigs. Four different microsatellite loci for species identification, along with six GM animal-specific fragments, were selected as targets for primer design. The capillary gel electrophoresis results of multiplex PCR showed that the target fragments were amplified successfully. This high-throughput multiplex PCR detection system can be applied for the inspection and quarantine of GM animals.

Sign in / Sign up

Export Citation Format

Share Document