Estimation of protein concentration at high sensitivity using SDS-capillary gel electrophoresis-laser induced fluorescence detection with 3-(2-furoyl)quinoline-2-carboxaldehyde protein labeling

2016 ◽  
Vol 37 (22) ◽  
pp. 2913-2921 ◽  
Author(s):  
Miriam S. Arrell ◽  
Franka Kálmán
Keyword(s):  
Fluorescence Detection ◽  
Gel Electrophoresis ◽  
Protein Concentration ◽  
High Sensitivity ◽  
Laser Induced Fluorescence ◽  
Protein Labeling ◽  
Capillary Gel Electrophoresis ◽  
Laser Induced Fluorescence Detection

Quantification of green fluorescent protein-(GFP-) tagged membrane proteins by capillary gel electrophoresis

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3648-3655
Author(s):  
Azeem Danish ◽  
Sang-Yong Lee ◽  
Christa E. Müller
Keyword(s):  
Green Fluorescent Protein ◽  
Membrane Proteins ◽  
Fluorescence Detection ◽  
Gel Electrophoresis ◽  
Fluorescent Protein ◽  
Laser Induced Fluorescence ◽  
Capillary Gel Electrophoresis ◽  
Laser Induced Fluorescence Detection ◽  
Green Fluorescent

A fast and robust procedure for the quantification of GFP-tagged membrane proteins in cell homogenates was developed employing capillary gel electrophoresis coupled to laser-induced fluorescence detection (CGE-LIF).

Sign in / Sign up

Export Citation Format

Share Document