Evaluation of Different Extraction Methods for the Analysis of Per‐ and Polyfluoroalkyl Substances (PFAS) in Dried Blood Spots from the Florida Manatee ( Trichechus manatus )

2021 ◽  
Author(s):  
Emily K. Griffin ◽  
Juan J. Aristizabal‐Henao ◽  
John A. Bowden
Keyword(s):  
Dried Blood Spots ◽  
Extraction Methods ◽  
Florida Manatee ◽  
Trichechus Manatus ◽  
Blood Spots ◽  
Polyfluoroalkyl Substances

scholarly journals Assessing Quality and Functionality of DNA from Fresh and Archival Dried Blood Spots and Recommendations for Quality Control Guidelines

2007 ◽  
Vol 53 (8) ◽  
pp. 1401-1407 ◽  
Author(s):  
Malin Ida Linnea Sjöholm ◽  
Joakim Dillner ◽  
Joyce Carlson
Keyword(s):  
Dna Extraction ◽  
Multiple Displacement Amplification ◽  
Dried Blood Spots ◽  
Extraction Methods ◽  
Population Based ◽  
Alkaline Lysis ◽  
Screening Programs ◽  
Blood Spots ◽  
Dna Yield ◽  
Chelex 100

Abstract Background: Dried blood spots (DBS) are a convenient and inexpensive method for biobanking. Although many countries have established population-based DBS biobanks from neonatal screening programs, the quality and usefulness of DNA from DBS have not been extensively assessed. Methods: We compared 4 common DNA extraction methods (Qiagen, EZNA, Chelex 100, and alkaline lysis) in a pilot study using fresh DBS with known lymphocyte count. We assessed suitability for multiple displacement amplification (MDA) and subsequent single-nucleotide polymorphism (SNP) analyses. We selected the EZNA method for DNA extraction from archival samples up to 27 years old, stored at room temperature or −20 °C, and SNP analyses were performed after MDA. Results: Extraction using alkaline lysis failed in most tests, and Chelex 100 was unsuccessful in real-time PCR, whereas the EZNA and Qiagen methods were successful by all evaluated quality indices. DNA extraction by EZNA, MDA, and SNP analyses were successful for the archival samples stored at −20 °C. Conclusion: Routine protocols for evaluation of the quality and functional integrity of DNA based on DNA yield, DNA size, and quantification of amplifiable DNA allow use of sufficient template for MDA and successful SNP analyses from both primary DBS extract and MDA product. A single 3-mm disc can yield sufficient DNA for several thousand SNP analyses. DNA from DBS is thus suitable for genetic epidemiology studies.

Evaluation of DNA extraction methods for dried blood spots in the diagnosis of congenital cytomegalovirus infection

2009 ◽  
Vol 46 ◽  
pp. S37-S42 ◽  
Author(s):  
Jutte J.C. de Vries ◽  
Eric C.J. Claas ◽  
Aloys C.M. Kroes ◽  
Ann C.T.M. Vossen
Keyword(s):  
Dna Extraction ◽  
Cytomegalovirus Infection ◽  
Dried Blood Spots ◽  
Extraction Methods ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Blood Spots ◽  
Dna Extraction Methods

scholarly journals Evaluation of DNA extraction methods for the detection of Cytomegalovirus in dried blood spots

2015 ◽  
Vol 66 ◽  
pp. 95-99 ◽  
Author(s):  
D. Koontz ◽  
K. Baecher ◽  
M. Amin ◽  
S. Nikolova ◽  
M. Gallagher ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Dried Blood Spots ◽  
Extraction Methods ◽  
Blood Spots ◽  
Dna Extraction Methods

scholarly journals The Effect of Storage and Extraction Methods on Amplification of Plasmodium falciparum DNA from Dried Blood Spots

2015 ◽  
Vol 92 (5) ◽  
pp. 922-925 ◽  
Author(s):  
Alanna Schwartz ◽  
Amrish Baidjoe ◽  
Philip J. Rosenthal ◽  
Teun Bousema ◽  
Bryan Greenhouse ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Dried Blood Spots ◽  
Extraction Methods ◽  
Blood Spots

Evaluation of rapid and sensitive DNA extraction methods for detection of cytomegalovirus in dried blood spots

2019 ◽  
Vol 265 ◽  
pp. 117-120 ◽  
Author(s):  
D. Koontz ◽  
S. Dollard ◽  
S. Cordovado
Keyword(s):  
Dna Extraction ◽  
Dried Blood Spots ◽  
Extraction Methods ◽  
Blood Spots ◽  
Dna Extraction Methods

Analysis of polyfluoroalkyl substances and bisphenol A in dried blood spots by liquid chromatography tandem mass spectrometry

2013 ◽  
Vol 405 (12) ◽  
pp. 4127-4138 ◽  
Author(s):  
Wanli Ma ◽  
Kurunthachalam Kannan ◽  
Qian Wu ◽  
Erin M. Bell ◽  
Charlotte M. Druschel ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Bisphenol A ◽  
Dried Blood Spots ◽  
Tandem Mass ◽  
Blood Spots ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Polyfluoroalkyl Substances

Touch, Hearing, and Vision of the Florida Manatee, Trichechus manatus latirostris

2010 ◽  
Author(s):  
Gordon B. Bauer ◽  
Joseph C. Gaspard ◽  
Debborah E. Colbert ◽  
Roger L. Reep ◽  
David Mann
Keyword(s):  
Florida Manatee ◽  
Trichechus Manatus ◽  
Trichechus Manatus Latirostris

Therapeutic drug monitoring (TDM) of the recent antipsychotic ziprasidone in dried blood spots (DBS) and plasma

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
L Mercolini ◽  
G Fulgenzi ◽  
M Melis ◽  
G Boncompagni ◽  
LJ Albers ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Dried Blood Spots ◽  
Therapeutic Drug ◽  
Blood Spots
Sign in / Sign up

Export Citation Format

Share Document