scholarly journals Statistical optimization of arachidonic acid synthesis by Mortierella alpina CBS 754.68 in a solid‐state fermenter

2021 ◽  
Author(s):  
Zahra Ghobadi ◽  
Zohreh Hamidi‐Esfahani ◽  
Mohammad Hossein Azizi
Keyword(s):  
Arachidonic Acid ◽  
Solid State ◽  
Acid Synthesis ◽  
Mortierella Alpina ◽  
Statistical Optimization

Arachidonic Acid Synthesis in Mortierella alpina: Origin, Evolution and Advancements

2016 ◽  
Vol 87 (4) ◽  
pp. 1053-1066
Author(s):  
Arun Chand Rayaroth ◽  
Rajesh Singh Tomar ◽  
Raghavendra Kumar Mishra
Keyword(s):  
Arachidonic Acid ◽  
Acid Synthesis ◽  
Mortierella Alpina

Biotechnology as a useful tool for nutritional improvement of cereal-based materials enriched with polyunsaturated fatty acids and pigments

2008 ◽  
Vol 56 (4) ◽  
pp. 377-384 ◽  
Author(s):  
M. čertík ◽  
Z. Adamechová ◽  
V. Hanusová ◽  
E. Breierová
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Solid State ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Linolenic Acid ◽  
Rhodotorula Glutinis ◽  
Mortierella Alpina ◽  
Nutritional Improvement ◽  
Sporobolomyces Roseus

The fungi Thamnidium elegans , as a producer of γ-linolenic acid (GLA), and Mortierella alpina , as a producer of dihomo-γ-linolenic acid (DGLA), arachidonic acid (AA) and eicosapentaenoic acid (EPA), and the yeasts Rhodotorula glutinis and Sporobolomyces roseus , as producers of ß-carotene, torulene and torularhodin, were tested for their ability to utilize cereal substrates during solid state fermentations (SSF). Depending on the strain and conditions, the cereal materials were effectively enriched with polyunsaturated fatty acids (PUFAs) or carotenoids. These naturally prepared bioproducts could find applications in food, feed, biomedical, pharmaceutical and veterinary fields.

The effect of pH, aeration, and temperature on arachidonic acid synthesis by Mortierella alpina

2015 ◽  
Vol 51 (2) ◽  
pp. 242-248 ◽  
Author(s):  
E. G. Dedyukhina ◽  
T. I. Chistyakova ◽  
A. A. Mironov ◽  
S. V. Kamzolova ◽  
I. G. Minkevich ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Acid Synthesis ◽  
Mortierella Alpina ◽  
Effect Of Ph

scholarly journals Metabolic Engineering of Mortierella alpina for Enhanced Arachidonic Acid Production through the NADPH-Supplying Strategy

2016 ◽  
Vol 82 (11) ◽  
pp. 3280-3288 ◽  
Author(s):  
Guangfei Hao ◽  
Haiqin Chen ◽  
Zhennan Gu ◽  
Hao Zhang ◽  
Wei Chen ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Fold Increase ◽  
Acid Synthesis ◽  
Pentose Phosphate ◽  
Mortierella Alpina ◽  
Enzymatic Reactions ◽  
Content Type ◽  
Glucose 6 Phosphate Dehydrogenase

ABSTRACTNADPH is known to be a key cofactor required for fatty acid synthesis and desaturation. Various enzymatic reactions can generate NADPH. To determine the effect of NADPH sources on lipogenesis, glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (PGD), isocitrate dehydrogenase (IDH), and malic enzyme (ME) were overexpressed inMortierella alpina. Our results showed that G6PD2 had the most significant effect on fatty acid synthesis, with a 1.7-fold increase in total fatty acid, whereas ME2 was more effective in desaturation, with a 1.5-fold increase in arachidonic acid (AA) content over control. Co-overexpression of G6PD2 and ME2 improved both fatty acid synthesis and desaturation. Within 96 h of fermentation using the fed-batch method, the co-overexpressing strain accumulated AA at a productivity of 1.9 ± 0.2 g/(liter · day), which was 7.2-fold higher than that in theM. alpinacontrol that was cultured in a flask.IMPORTANCEThis study proved that the pentose phosphate pathway is the major NADPH contributor during fatty acid synthesis inM. alpina. The NADPH sources may be differently responsible for fatty acid synthesis or desaturation. Co-overexpression of G6PD2 and ME2 significantly increases AA production.

scholarly journals Arachidonic acid synthesis studied in isolated liver cells

FEBS Letters ◽  
1981 ◽  
Vol 133 (2) ◽  
pp. 201-204 ◽  
Author(s):  
B.O. Christophersen ◽  
Jon Norseth
Keyword(s):  
Arachidonic Acid ◽  
Acid Synthesis ◽  
Liver Cells ◽  
Isolated Liver Cells ◽  
Isolated Liver

Polyunsaturated fatty acids production by solid‐state fermentation on polyurethane foam by Mortierella alpina

2021 ◽  
Author(s):  
Marta Ferreira ◽  
Helena Fernandes ◽  
Helena Peres ◽  
Aires Oliva‐Teles ◽  
Isabel Belo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Solid State ◽  
Polyunsaturated Fatty Acids ◽  
Polyurethane Foam ◽  
Solid State Fermentation ◽  
Mortierella Alpina
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