Cell ablation using wild-type and cold-sensitive ricin-a chain indrosophila embryonic mesoderm

genesis ◽  
2002 ◽  
Vol 34 (1-2) ◽  
pp. 132-134 ◽  
Author(s):  
Marcus J. Allen ◽  
Cahir J. O'Kane ◽  
Kevin G. Moffat
Keyword(s):  
Wild Type ◽  
Ricin A Chain ◽  
Cell Ablation ◽  
Cold Sensitive ◽  
A Chain ◽  
Ricin A

scholarly journals Ribosome inactivation by ricin A chain: a sensitive method to assess the activity of wild-type and mutant polypeptides.

1989 ◽  
Vol 8 (1) ◽  
pp. 301-308 ◽  
Author(s):  
M. J. May ◽  
M. R. Hartley ◽  
L. M. Roberts ◽  
P. A. Krieg ◽  
R. W. Osborn ◽  
...  
Keyword(s):  
Wild Type ◽  
Ricin A Chain ◽  
Ribosome Inactivation ◽  
A Chain ◽  
Mutant Polypeptides ◽  
Ricin A ◽  
Sensitive Method

scholarly journals Functional divergence between the two P1–P2 stalk dimers on the ribosome in their interaction with ricin A chain

2014 ◽  
Vol 460 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Przemysław Grela ◽  
Xiao-Ping Li ◽  
Marek Tchórzewski ◽  
Nilgun E. Tumer
Keyword(s):  
Functional Divergence ◽  
Wild Type ◽  
Ricin A Chain ◽  
Yeast Ribosomes ◽  
A Chain ◽  
Wild Type Yeast ◽  
Ricin A

Wild-type yeast ribosomes and ribosomes bearing only the P1B–P2A dimer bound to the ricin A chain better and were more susceptible to depurination and toxicity than ribosomes bearing only the P1A–P2B dimer, indicating that the two stalk dimers differ in their function.

scholarly journals Inducible cell ablation in Drosophila by cold-sensitive ricin A chain

Development ◽  
1992 ◽  
Vol 114 (3) ◽  
pp. 681-687 ◽  
Author(s):  
K.G. Moffat ◽  
J.H. Gould ◽  
H.K. Smith ◽  
C.J. O'Kane
Keyword(s):  
Single Amino Acid ◽  
Temperature Sensitive ◽  
Temperature Dependent ◽  
Ricin Toxin ◽  
Cell Ablation ◽  
Active Site Cleft ◽  
Cold Sensitive ◽  
Low Toxicity ◽  
A Chain ◽  
Ricin A

We have developed a system for temperature-inducible killing of specific cells in the fruitfly Drosophila melanogaster. The system overcomes many of the limitations of existing cell ablation methods and is in principle applicable to any non-homeothermic eukaryote. Temperature-sensitive and cold-sensitive mutations in the ricin toxin A chain (RTA) of castor bean were generated in yeast. One cold-sensitive mutation, RAcs2, produced temperature-dependent ablation of eye cells in Drosophila when expressed under control of the eye-specific sev enhancer. At 29 degrees C, cell death was observed within 7 hours in the developing eye and no obvious toxic effects were observed elsewhere; at 18 degrees C, extremely low toxicity was observed. DNA sequencing of RAcs2 revealed a single amino acid substitution in the RTA active site cleft.

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