Specificity and sensitivity of gp210 autoantibodies detected using an enzyme-linked immunosorbent assay and a synthetic polypeptide in the diagnosis of primary biliary cirrhosis

Enzyme-linked immunosorbent assay (ELISA) with high specificity and sensitivity is one of the most popular techniques for detecting carbendazim (CBD), a commonly used benzimidazole fungicide in agriculture. However, the traditional...

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An enzyme-linked immunosorbent assay for the detection of IgG antibodies against Babesia equi in horses

Ciência Rural ◽

10.1590/s0103-84782004000500031 ◽

2004 ◽

Vol 34 (5) ◽

pp. 1525-1529 ◽

Cited By ~ 12

Author(s):

Cristiane Divan Baldani ◽

Rosangela Zacarias Machado ◽

Paulo de Tarso Landgraf Botteon ◽

Felipe Santoro Takakura ◽

Carlos Luiz Massard

Keyword(s):

Immunosorbent Assay ◽

Serological Test ◽

Epidemiological Studies ◽

Sao Paulo ◽

Enzyme Linked Immunosorbent Assay ◽

São Paulo ◽

Igg Antibodies ◽

Serum Samples ◽

Babesia Equi ◽

Specificity And Sensitivity

A crude antigenic preparation of Babesia equi was used to develop and establish the suitability of an enzyme-linked immunosorbent assay (ELISA) for the detection of parasite carriers. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkboard titrations. The specificity and sensitivity of the ELISA were 100 %. A total of 90 serum samples were taken from horses from the Northeast region of São Paulo State and examined for diagnosis of equine B. equi infection by ELISA. Approximately 75% (n=67) of all the horses tested were found serologically positive for B. equi. These results suggest that the ELISA described may prove to be an appropriate serological test for epidemiological studies on B. equi infections in the field and that equine piroplasmosis is a cause for serious concern in the State of São Paulo, Brazil.

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Seven Different Assays of Hyaluronan Compared for Clinical Utility

Clinical Chemistry ◽

10.1093/clinchem/38.1.127 ◽

1992 ◽

Vol 38 (1) ◽

pp. 127-132 ◽

Cited By ~ 34

Author(s):

Ulla Lindqvist ◽

Kenji Chichibu ◽

Bertrand Delpech ◽

Ronald L Goldberg ◽

Warren Knudson ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Primary Biliary Cirrhosis ◽

Clinical Utility ◽

High Specificity ◽

The Other ◽

Healthy Population ◽

Biliary Cirrhosis ◽

Specificity And Sensitivity ◽

Clinical Investigations ◽

Healthy Persons

Abstract To compare six assays of hyaluronan (hyaluronic acid; HYA) in serum, developed in different laboratories, we analyzed 10 samples from each of three groups: healthy persons, patients with primary biliary cirrhosis, and patients with rheumatoid arthritis. All the assays are based on the use of affinity proteins specific for HYA, prepared from cartilage or brain tissue, and are analogous to RIA or enzyme immunoassay techniques. The assay results were of the same magnitude. Although statistical analysis indicated that the methods in some cases deviated significantly from one another, this variation was less than the physiological variation in the healthy population. Therefore, the results of clinical investigations in which the various methods have been used are comparable. The analyses have high specificity and sensitivity for primary biliary cirrhosis but are somewhat less suitable for detecting rheumatoid arthritis. A seventh laboratory, which obtained antibodies to HYA, used these in an RIA to analyze a separate series of serum specimens. Results were in agreement with those obtained by one of the other assays.

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Development and preliminary application of gE enzyme-linked immunosorbent assay for detection of the antibody to gE protein ofPseudorabies virusin pigs

Chinese Journal of Agricultural Biotechnology ◽

10.1079/cjb200561 ◽

2005 ◽

Vol 2 (2) ◽

pp. 79-83 ◽

Cited By ~ 1

Author(s):

Tang Yong ◽

Chen Huan-Chun ◽

Qin Ya-Li ◽

He Qi-Gai ◽

Jin Mei-Lli ◽

...

Keyword(s):

Immunosorbent Assay ◽

Pseudorabies Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Diagnostic Specificity ◽

Serum Samples ◽

Agreement Rate ◽

Glycoprotein E ◽

Recombinant Glycoprotein ◽

Specificity And Sensitivity ◽

Preliminary Application

AbstractTo differentiate pigs infected withPseudorabies virus(PrV) from pigs vaccinated with gE-PrV, a glycoprotein E enzyme-linked immunosorbent assay (gE-ELISA) based on recombinant glycoprotein E (gE) (which was expressed byEscherichia coli, purified, denatured and renatured) was developed. By testing 115 serum samples, the diagnostic specificity and sensitivity of the developed gE-ELISA were evaluated to be 94.5% and 96.7%, respectively. Five serum samples were tested with plates from five lots, and the results had a coefficient of variation of less than 10%, showing good reproducibility of gE-ELISA. This gE-ELISA was compared with a commercial blocking ELISA by testing 356 serum samples. The agreement rate of the two assays was 92.13% (328/356). These results suggested that the gE-ELISA developed in our laboratory could be used in differentiating PrV-infected and gE-PrV-vaccinated pigs.

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Application of Competitive Enzyme-Linked Immunosorbent Assay for the Serologic Diagnosis of Classical Swine Fever Virus Infection

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870101300416 ◽

2001 ◽

Vol 13 (4) ◽

pp. 357-360 ◽

Cited By ~ 13

Author(s):

Alfonso Clavijo ◽

Min Lin ◽

José Riva ◽

En-Min Zhou

Keyword(s):

Immunosorbent Assay ◽

Classical Swine Fever Virus ◽

Classical Swine Fever ◽

Fever Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Prevalence Studies ◽

Specificity And Sensitivity ◽

Kappa Value ◽

Relative Specificity ◽

Level Of Agreement

A competitive enzyme-linked immunosorbent assay (C-ELISA), based on a truncated E2 recombinant protein of the Alfort/187 strain of classical swine fever virus (CSFV) and a specific monoclonal antibody M1669, was evaluated using 2,000 sera from clinically healthy pigs in Canada (a CSFV-free country) and sera from experimentally infected pigs. The relative specificity and sensitivity of the C-ELISA were 100% and 86%, respectively, at a cutoff of 25% inhibition using negative and positive pig sera, as defined by the neutralizing peroxidase-linked assay (NPLA). A kappa value of 0.91 was obtained, indicating an excellent level of agreement between the NPLA and the C-ELISA. When sera from 120 infected pigs were used in the test at ≥21 days postinfection, the sensitivity of the C-ELISA and the kappa value increased to 97% and 0.98, respectively. This C-ELISA will be useful when a large number of samples must be tested, as could occur during a disease outbreak or for surveillance or prevalence studies.

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