Phenotypic characterization of ‘non-T, non-B’ acute lymphoblastic leukemia by a new panel (BL) of monoclonal antibodies

1985 ◽  
Vol 3 (4) ◽  
pp. 271-281 ◽  
Author(s):  
Ayad Al-Katib ◽  
Chang Yi Wang ◽  
Ricardo Bardales ◽  
Benjamin Koziner
Keyword(s):  
Monoclonal Antibodies ◽  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Phenotypic Characterization

scholarly journals Cell surface characterization of malignant T cells from lymphoblastic lymphoma using monoclonal antibodies: evidence for phenotypic differences between malignant T cells from patients with acute lymphoblastic leukemia and lymphoblastic lymphoma

Blood ◽  
1981 ◽  
Vol 57 (6) ◽  
pp. 1105-1110 ◽  
Author(s):  
A Bernard ◽  
L Boumsell ◽  
EL Reinherz ◽  
LM Nadler ◽  
J Ritz ◽  
...  
Keyword(s):  
T Cells ◽  
Monoclonal Antibodies ◽  
Acute Lymphoblastic Leukemia ◽  
Surface Characterization ◽  
Lymphoblastic Leukemia ◽  
Lymphoblastic Lymphoma ◽  
Lymphoid Cells ◽  
Phenotypic Differences ◽  
Malignant T Cells

A series of monoclonal antibodies was used for the characterization of malignant T cells from 21 patients with lymphoblastic lymphoma (LL). The tumor population from these patients showed a marked degree of phenotypic heterogeneity and a proportion (one-third) of patients had tumor cells that did not conform exactly with the cells normally detected in the thymus. However, these cell populations could be related to the early or common or late thymocyte population (about one- third of the patients in each category). This contrast, with the characterization of malignant T cells from 43 patients with acute lymphoblastic leukemia (ALL) that could be related to either early or common thymocytes, with an exception of two patients categorized as having a tumor population related to late thymocytes. Further phenotypic differences between cells from ALL and LL could be demonstrated by investigation with two additional monoclonal antibodies, A50 and U4. Among patients with malignant T cells related to common thymocyte, 0/12 patients with ALL had cells recognized by A50, where 5/8 patients with LL had A50+ cells. Among patients with early thymocytes, only patients with ALL had cells recognized by U4. In addition, 5 LL patients had cells reactive with J5, a monoclonal antibody recognizing the common ALL antigen (CALLA). Since CALLA was found on cells related to common and late thymocytes, CALLA is neither lineage specific, nor can it be viewed as being peculiar to malignant lymphoid cells arrested at very immature stages of differentiation.

scholarly journals Cell surface characterization of malignant T cells from lymphoblastic lymphoma using monoclonal antibodies: evidence for phenotypic differences between malignant T cells from patients with acute lymphoblastic leukemia and lymphoblastic lymphoma

Blood ◽  
1981 ◽  
Vol 57 (6) ◽  
pp. 1105-1110 ◽  
Author(s):  
A Bernard ◽  
L Boumsell ◽  
EL Reinherz ◽  
LM Nadler ◽  
J Ritz ◽  
...  
Keyword(s):  
T Cells ◽  
Monoclonal Antibodies ◽  
Acute Lymphoblastic Leukemia ◽  
Surface Characterization ◽  
Lymphoblastic Leukemia ◽  
Lymphoblastic Lymphoma ◽  
Lymphoid Cells ◽  
Phenotypic Differences ◽  
Malignant T Cells

Abstract A series of monoclonal antibodies was used for the characterization of malignant T cells from 21 patients with lymphoblastic lymphoma (LL). The tumor population from these patients showed a marked degree of phenotypic heterogeneity and a proportion (one-third) of patients had tumor cells that did not conform exactly with the cells normally detected in the thymus. However, these cell populations could be related to the early or common or late thymocyte population (about one- third of the patients in each category). This contrast, with the characterization of malignant T cells from 43 patients with acute lymphoblastic leukemia (ALL) that could be related to either early or common thymocytes, with an exception of two patients categorized as having a tumor population related to late thymocytes. Further phenotypic differences between cells from ALL and LL could be demonstrated by investigation with two additional monoclonal antibodies, A50 and U4. Among patients with malignant T cells related to common thymocyte, 0/12 patients with ALL had cells recognized by A50, where 5/8 patients with LL had A50+ cells. Among patients with early thymocytes, only patients with ALL had cells recognized by U4. In addition, 5 LL patients had cells reactive with J5, a monoclonal antibody recognizing the common ALL antigen (CALLA). Since CALLA was found on cells related to common and late thymocytes, CALLA is neither lineage specific, nor can it be viewed as being peculiar to malignant lymphoid cells arrested at very immature stages of differentiation.

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

scholarly journals Comprehensive genomic characterization of gene therapy-induced T-cell acute lymphoblastic leukemia

Leukemia ◽  
2020 ◽  
Vol 34 (10) ◽  
pp. 2785-2789
Author(s):  
Peter Horak ◽  
Sebastian Uhrig ◽  
Maximilian Witzel ◽  
Irene Gil-Farina ◽  
Barbara Hutter ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Acute Lymphoblastic Leukemia ◽  
T Cell ◽  
Lymphoblastic Leukemia ◽  
Genomic Characterization ◽  
Cell Acute Lymphoblastic Leukemia

Characterization of acute lymphoblastic leukemia subtypes in Brazilian patients

1996 ◽  
Vol 20 (4) ◽  
pp. 349-355 ◽  
Author(s):  
Eduardo M. Rego ◽  
Aglair B. Garcia ◽  
Sonia R. Viana ◽  
Roberto P. Falcão
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia

scholarly journals Purification and Characterization of Asparaginase fromPhaseolus vulgarisSeeds

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Saleh A. Mohamed ◽  
Mohamed F. Elshal ◽  
Taha A. Kumosani ◽  
Alia M. Aldahlawi
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Potential Candidate ◽  
Purification And Characterization ◽  
Apparent Homogeneity ◽  
Wide Range ◽  
Optimum Ph ◽  
Glutaminase Activity ◽  
Low Activity

L-asparaginase from bacteria has been used in treatment of acute lymphoblastic leukemia. The aim of this study was to purify and characterize L-asparaginase fromPhaseolus vulgarisseeds instead of microbial sources. L-asparaginase was purified to apparent homogeneity. The enzyme has molecular mass of 79 kDa. The purified asparaginase had very low activity toward a number of asparagine and glutamine analogues. L-asparaginase was free from glutaminase activity. Kinetic parameters, Km andVmax of purified enzyme, were found to be 6.72 mM and 0.16 μM, respectively. The enzyme had optimum pH at 8.0. The enzyme showed high stability at alkaline pH (pH 7.5–9.0) when incubated for up to 24 h. L-asparaginase had the same temperature optimum and thermal stability at 37°C. K+was able to greatly enhance the activity of asparaginase by 150% compared with other metals tested. In conclusion, L-asparaginase showed no glutaminase activity and good stability over a wide range of physiological conditions, and thus it could be used as a potential candidate for treatment of acute lymphoblastic leukemia.

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