scholarly journals Neuropilin-1 is differentially expressed in myoepithelial cells and vascular smooth muscle cells in preneoplastic and neoplastic human breast: A possible marker for the progression of breast cancer

2002 ◽  
Vol 101 (5) ◽  
pp. 409-414 ◽  
Author(s):  
John M. Stephenson ◽  
Snigdha Banerjee ◽  
Neela K. Saxena ◽  
Rachel Cherian ◽  
Sushanta K. Banerjee
Keyword(s):  
Breast Cancer ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Myoepithelial Cells ◽  
Differentially Expressed ◽  
Human Breast ◽  
Neuropilin 1

Breast cancer cells secreted platelet-derived growth factor-induced motility of vascular smooth muscle cells is mediated through neuropilin-1

2006 ◽  
Vol 45 (11) ◽  
pp. 871-880 ◽  
Author(s):  
Snigdha Banerjee ◽  
Krishanu Sengupta ◽  
Kakali Dhar ◽  
Smita Mehta ◽  
Patricia A. D'Amore ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Smooth Muscle ◽  
Growth Factor ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cancer Cells ◽  
Vascular Smooth Muscle Cells ◽  
Breast Cancer Cells ◽  
Platelet Derived Growth Factor ◽  
Neuropilin 1

scholarly journals GRIA2/ENPP3 Regulates the Proliferation and Migration of Vascular Smooth Muscle Cells in the Restenosis Process Post-PTA in Lower Extremity Arteries

2021 ◽  
Vol 12 ◽  
Author(s):  
Mi Zhou ◽  
Lixing Qi ◽  
Yongquan Gu
Keyword(s):  
Smooth Muscle ◽  
Lower Extremity ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Enrichment Analysis ◽  
Muscle Cells ◽  
Differentially Expressed ◽  
And Migration ◽  
Proliferation And Migration

Restenosis is the main restriction on the long-term efficacy of percutaneous transluminal angioplasty (PTA) therapy for peripheral artery disease (PAD). Interventions to prevent restenosis are poor, and the exact mechanism is unclear. Here, we aimed to elucidate the role of GRIA2 in the restenosis process post-PTA in lower extremity arteries. We searched the differentially expressed genes (DEGs) between atherosclerotic and restenotic artery plaques in the Gene Expression Omnibus (GEO), and five DEGs were identified. Combined with Gene Ontology (GO) enrichment analysis, GRIA2 was significantly correlated with the restenosis process. Tissue samples were used to examine GRIA2 expression by immunofluorescence staining of atherosclerotic and restenotic artery plaques. The regulation of GRIA2 in vascular smooth muscle cells (VSMCs) was confirmed by lentiviral transfection. Overexpression of GRIA2 promoted the proliferation and migration of VSMCs. Using Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis and protein–protein interaction (PPI) network, a strong connection between ENPP3 and GRIA2 was discovered. In vitro results showed that the high expression of GRIA2 in VSMCs enhanced the expression of ENPP3, while downregulation of GRIA2 downregulated ENPP3. GRIA2 is highly differentially expressed in restenotic arterial plaques, promoting the proliferation and migration of VSMCs through upregulation of ENPP3. These discoveries will help us to obtain a better understanding of restenosis in lower extremity arteries.

scholarly journals A fibroblast-associated antigen: characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells.

1992 ◽  
Vol 40 (4) ◽  
pp. 475-486 ◽  
Author(s):  
L Rønnov-Jessen ◽  
J E Celis ◽  
B Van Deurs ◽  
O W Petersen
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Stromal Cells ◽  
Breast Tissue ◽  
Muscle Cells ◽  
Human Breast ◽  
Human Breast Tissue ◽  
Coated Pits

Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle cells. The antigen was detected on the cell surface and in cathepsin D-positive and acridine orange-accumulating vesicular compartments of fibroblasts. Ultrastructurally, the antigen was revealed in coated pits and in endosomal and lysosomal structures. 1B10 recognized three major brands migrating at apparent Mr of 38,000, 45,000, and 80,000, in addition to many minor bands between Mr 45,000 and 97,000, including Mr 52,000. The Mr 45,000 and 38,000 were associated with the cell membrane and Mr 52,000 as well as Mr 38,000 were associated with the lysosomes. The 1B10 immunoreactivity was specific to fibroblasts and smooth muscle differentiated fibroblasts within the context of vascular smooth muscle cells.

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