scholarly journals A highly specific real-time RT-PCR method for the quantitative determination of CK-19 mRNA positive cells in peripheral blood of patients with operable breast cancer

2006 ◽  
Vol 119 (7) ◽  
pp. 1654-1659 ◽  
Author(s):  
Aliki Stathopoulou ◽  
Maria Ntoulia ◽  
Maria Perraki ◽  
Stella Apostolaki ◽  
Dimitris Mavroudis ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Quantitative Determination ◽  
Real Time ◽  
Peripheral Blood ◽  
Operable Breast Cancer ◽  
Rt Pcr ◽  
Pcr Method

scholarly journals RT-PCR determination of maspin and mammaglobin B in peripheral blood of healthy donors and breast cancer patients

2006 ◽  
Vol 17 (3) ◽  
pp. 424-428 ◽  
Author(s):  
L. Mercatali ◽  
V. Valenti ◽  
D. Calistri ◽  
S. Calpona ◽  
G. Rosti ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Breast Cancer Patients ◽  
Rt Pcr ◽  
Healthy Donors

scholarly journals Cyclin E Expression in Operable Breast Cancer Quantified Using Real-Time RT–PCR: A Comparative Study with Immunostaining

2005 ◽  
Vol 36 (3) ◽  
pp. 142-149 ◽  
Author(s):  
Piotr Potemski ◽  
Elzbieta Pluciennik ◽  
Andrzej K. Bednarek ◽  
Renata Kusinska ◽  
Dorota Jesionek-Kupnicka ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Comparative Study ◽  
Real Time ◽  
Cyclin E ◽  
Operable Breast Cancer ◽  
Rt Pcr

Real-time RT-PCR of CD146 and VE-cadherin mRNA to detect circulating endothelial cells in peripheral blood of patients with breast cancer

2006 ◽  
Vol 4 (2) ◽  
pp. 126
Author(s):  
I. Van der Auwera ◽  
Elst ◽  
V. Huygelen ◽  
S. Van Laere ◽  
G. Van den Eynden ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Endothelial Cells ◽  
Real Time ◽  
Peripheral Blood ◽  
Circulating Endothelial Cells ◽  
Rt Pcr

scholarly journals Correlation of ER, PR, and HER2 at protein and mRNA levels in the Asian patients with operable breast cancer

2022 ◽  
Author(s):  
Chih-Jung Chen ◽  
Ting-Hao Chen ◽  
Jason Lei ◽  
Ji-An Liang ◽  
Po-Sheng Yang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Growth Factor Receptor ◽  
Kappa Statistic ◽  
Operable Breast Cancer ◽  
Current Evidence ◽  
Mrna Levels ◽  
Breast Cancer Patients ◽  
Rt Pcr ◽  
Asian Patients ◽  
Pcr Method

Breast cancer is the most common cancer and the leading cause of cancer-related death in women. The estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) are important biomarkers in the prognosis of breast cancer, and their expression is used to categorize breast cancer into subtypes. We aimed to analyze the concordance between ER, PR, and HER2 expression levels and breast cancer subtyping results obtained by immunohistochemistry (IHC, for protein) and reverse transcriptase-polymerase chain reaction (RT-PCR, for mRNA) and to assess the recurrence-free survival (RFS) of the different subtypes as determined by the two methods. We compared biomarker expression by IHC and RT-PCR in 397 operable breast cancer patients and categorized all patients into luminal, HER2, and triple-negative (TN) subtypes. The concordance of biomarker expression between the two methods was 81.6% (kappa = 0.4075) for ER, 87.2% (kappa = 0.5647) for PR, and 79.1% (kappa = 0.2767) for HER2. The kappa statistic was 0.3624 for the resulting luminal, HER2, and TN subtypes. The probability of a 5-year RFS was 0.78 for the luminal subtype versus 0.77 for HER2 and 0.51 for TN, when determined by IHC (p = 0.007); and 0.80, 0.71, and 0.61, respectively, when determined by the RT-PCR method (p = 0.008). Based on the current evidence, subtyping by RT-PCR performs similarly to conventional IHC with regard to the 5-year prognosis. The PCR method may thus provide a complementary means of subtyping when IHC results are ambiguous.

Real-time RT-PCR of CD146 and VE-cadherin mRNA to detect circulating endothelial cells in peripheral blood of patients with breast cancer

2006 ◽  
Vol 4 (2) ◽  
pp. 144 ◽  
Author(s):  
L. Dirix ◽  
Van der Auwera ◽  
H. Elst ◽  
G. Van den Eynden ◽  
V. Huygelen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Endothelial Cells ◽  
Real Time ◽  
Peripheral Blood ◽  
Circulating Endothelial Cells ◽  
Rt Pcr

scholarly journals Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA

2017 ◽  
Vol 11 (07) ◽  
pp. 543-548
Author(s):  
Sibel Aydogan ◽  
Ziya Cibal Acikgoz ◽  
Aysegul Gozalan ◽  
Fisun Kirca ◽  
Tuba Muderris ◽  
...  
Keyword(s):  
Quality Control ◽  
Real Time ◽  
Hbv Dna ◽  
Clinical Samples ◽  
Hcv Rna ◽  
Rt Pcr ◽  
B Virus ◽  
Pcr Method ◽  
Polymerase Chain

Introduction: The purpose of this study was to evaluate and compare two manual isolation and real-time (RT) polymerase chain reaction (PCR) kits (RTA RT-PCR with RTA isolation kit and Artus RG RT-PCR with QIAamp isolation kit) for molecular diagnosis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. Methodology: The study was conducted on 121 and 54 clinical samples for the detection of HBV DNA and HCV RNA, respectively, with an additional 8 HCV RNA external quality control samples. Results: Though a high correlation was observed between the two kits for the HBV DNA (r = 0.955, p = 0.001) and HCV RNA quantifications (r = 0.828, p = 0.001), discordant results were found in nine of the HBV DNA and in six of the HCV RNA samples. The mean difference between the two systems was found to be 0.4 log IU/mL in Quality Control for Molecular Diagnostics (QCMD) HCV RNA samples by Bland-Altman analysis. Conclusions: Although there was a high correlation between HBV DNA and HCV RNA tests according to the results of the study, the RTA system requires improvement for the determination of HCV RNA.

scholarly journals METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS

2018 ◽  
pp. 31-35
Author(s):  
M. I. Doronin ◽  
A. M. Timina ◽  
D. A. Lozovoy ◽  
V. A. Starikov ◽  
D. V. Mikhalishin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Regression Model ◽  
Real Time ◽  
Reverse Transcription ◽  
Raw Materials ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Method ◽  
Polymerase Chain

Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. Negative regression model of  С146S = (30.269 – Ct)/4.155 type allowing determination of FMDV 146S particle concentrations based on the amplification threshold cycle values (Ct) is proposed. It has been experimentally proven that the quantity of the 146S component determined by the real-time RT-PCR method using developed regression model and contained in the inoculation dose of FMD vaccine confers protection to the vaccinated animals against generalized FMD of A, O, Asia-1 types. rtRT-PCR method is highly sensitive and allows rapid and highly reliable estimation of  the 146S antigen concentration in FMD vaccine. The method for 146S particle quantity determination by real-time RT-PCR using the regression model is reliable and demonstrates high correlation (95.5–99.0%) with the complement fixation test results.

A multiplex real-time PCR method for the quantitative determination of equine (horse) fractions in meat products

Food Control ◽  
2017 ◽  
Vol 74 ◽  
pp. 89-97 ◽  
Author(s):  
A. Iwobi ◽  
D. Sebah ◽  
G. Spielmann ◽  
M. Maggipinto ◽  
M. Schrempp ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Meat Products ◽  
Pcr Method
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