Solute carrier family 16 member 5 downregulation and its methylation might serve as a prognostic indicator of prostate cancer

AbstractKinesins play important roles in the progression and development of cancer. Kinesin family member C1 (KIFC1), a minus end-directed motor protein, is a novel Kinesin involved in the clustering of excess centrosomes found in cancer cells. Recently KIFC1 has shown to play a role in the progression of many different cancers, however, the involvement of KIFC1 in the progression of prostate cancer (PCa) is still not well understood. This study investigated the expression and clinical significance of KIFC1 in PCa by utilizing multiple publicly available datasets to analyze KIFC1 expression in patient samples. High KIFC1 expression was found to be associated with high Gleason score, high tumor stage, metastatic lesions, high ploidy levels, and lower recurrence-free survival. These results reveal that high KIFC1 levels are associated with a poor prognosis for PCa patients and could act as a prognostic indicator for PCa patients as well.

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Autosomal recessive congenital hereditary corneal dystrophy associated with a novel SLC4A11 mutation in two consanguineous Tunisian families

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2020-318204 ◽

2021 ◽

pp. bjophthalmol-2020-318204

Author(s):

Zohra Chibani ◽

Imen Zone Abid ◽

Peter Söderkvist ◽

Jamel Feki ◽

Mounira Hmani Aifa

Keyword(s):

Autosomal Recessive ◽

Corneal Transplantation ◽

Gene Mutations ◽

In Silico Analysis ◽

Corneal Dystrophy ◽

Solute Carrier Family ◽

Transporter Protein ◽

Bicarbonate Transporter ◽

Corneal Clouding ◽

Solute Carrier

BackgroundAutosomal recessive congenital hereditary corneal dystrophy (CHED) is a rare isolated developmental anomaly of the eye characterised by diffuse bilateral corneal clouding that may lead to visual impairment requiring corneal transplantation. CHED is known to be caused by mutations in the solute carrier family 4 member 11 (SLC4A11) gene which encodes a membrane transporter protein (sodium bicarbonate transporter-like solute carrier family 4 member 11).MethodsTo identify SLC4A11 gene mutations associated with CHED (OMIM: #217700), genomic DNA was extracted from whole blood and sequenced for all exons and intron-exon boundaries in two large Tunisian families.ResultsA novel deletion SLC4A11 mutation (p. Leu479del; c.1434_1436del) is responsible for CHED in both analysed families. This non-frameshift mutation was found in a homozygous state in affected members and heterozygous in non-affected members. In silico analysis largely support the pathogenicity of this alteration that may leads to stromal oedema by disrupting the osmolarity balance. Being localised to a region of alpha-helical secondary structure, Leu479 deletion may induce protein-compromising structural rearrangements.ConclusionTo the best of our knowledge, this is the first clinical and genetic study exploring CHED in Tunisia. The present work also expands the list of pathogenic genotypes in SLC4A11 gene and its associated clinical diagnosis giving more insights into genotype–phenotype correlations.

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Slc7a11 Modulated by POU2F1 is Involved in Pigmentation in Rabbit

International Journal of Molecular Sciences ◽

10.3390/ijms20102493 ◽

2019 ◽

Vol 20 (10) ◽

pp. 2493 ◽

Cited By ~ 4

Author(s):

Yang Chen ◽

Shuaishuai Hu ◽

Lin Mu ◽

Bohao Zhao ◽

Manman Wang ◽

...

Keyword(s):

Skin Color ◽

Site Directed Mutagenesis ◽

Molecular Regulation ◽

Luciferase Reporter ◽

Directed Mutagenesis ◽

Regulation Mechanism ◽

Solute Carrier Family ◽

Depth Analysis ◽

Solute Carrier ◽

Fur Color

Solute carrier family 7 member 11 (Slc7a11) is a cystine/glutamate xCT transporter that controls the production of pheomelanin pigment to change fur and skin color in animals. Previous studies have found that skin expression levels of Slc7a11 varied significantly with fur color in Rex rabbits. However, the molecular regulation mechanism of Slc7a11 in pigmentation is unknown. Here, rabbit melanocytes were first isolated and identified. The distribution and expression pattern of Slc7a11 was confirmed in skin from rabbits with different fur colors. Slc7a11 affected the expression of pigmentation related genes and thus affected melanogenesis. Meanwhile, Slc7a11 decreased melanocyte apoptosis, but inhibition of Slc7a11 enhanced apoptosis. Furthermore, the POU2F1 protein was found to bind to the −713 to −703 bp region of Slc7a11 promoter to inhibit its activity in a dual-luciferase reporter and site-directed mutagenesis assay. This study reveals the function of the Slc7a11 in melanogenesis and provides in-depth analysis of the mechanism of fur pigmentation.

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The solute carrier family 10 (SLC10): Beyond bile acid transport

Molecular Aspects of Medicine ◽

10.1016/j.mam.2012.07.004 ◽

2013 ◽

Vol 34 (2-3) ◽

pp. 252-269 ◽

Cited By ~ 73

Author(s):

Tatiana Claro da Silva ◽

James E. Polli ◽

Peter W. Swaan

Keyword(s):

Bile Acid ◽

Solute Carrier Family ◽

Acid Transport ◽

Bile Acid Transport ◽

Solute Carrier

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The human solute carrier family 11 member 1 protein (SLC11A1): linking infections, autoimmunity and cancer?

FEMS Immunology & Medical Microbiology ◽

10.1111/j.1574-695x.2007.00231.x ◽

2007 ◽

Vol 49 (3) ◽

pp. 324-329 ◽

Cited By ~ 20

Author(s):

Agnes A. Awomoyi

Keyword(s):

Solute Carrier Family ◽

Solute Carrier

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A Nonsense Polymorphism (Y319X) of the Solute Carrier Family 6 Member 18 (SLC6A18) Gene is Not Associated with Hypertension and Blood Pressure in Japanese

The Tohoku Journal of Experimental Medicine ◽

10.1620/tjem.208.25 ◽

2006 ◽

Vol 208 (1) ◽

pp. 25-31 ◽

Cited By ~ 15

Author(s):

Bita Eslami ◽

Masato Kinboshi ◽

Sumiko Inoue ◽

Kouji Harada ◽

Kayoko Inoue ◽

...

Keyword(s):

Blood Pressure ◽

Solute Carrier Family ◽

Solute Carrier

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Downregulation and Prognostic Performance of MicroRNA 224 Expression in Prostate Cancer

Clinical Chemistry ◽

10.1373/clinchem.2012.191502 ◽

2013 ◽

Vol 59 (1) ◽

pp. 261-269 ◽

Cited By ~ 44

Author(s):

Konstantinos Mavridis ◽

Konstantinos Stravodimos ◽

Andreas Scorilas

Keyword(s):

Prostate Cancer ◽

Cox Regression ◽

Specific Antigen ◽

Progression Free Survival ◽

Prognostic Indicator ◽

Disease Stage ◽

Clinical Value ◽

Prostate Tumors ◽

Tissue Samples ◽

Total Rna

INTRODUCTION The extensive use of prostate-specific antigen as a general prostate cancer biomarker has introduced the hazards of overdiagnosis and overtreatment. Recent studies have revealed the immense biomarker capacity of microRNAs (miRNAs) in prostate cancer. The aim of this study was to analyze the expression pattern of miR-224, a cancer-related miRNA, in prostate tumors and investigate its clinical utility. METHODS Total RNA was isolated from 139 prostate tissue samples. After the polyadenylation of total RNA by poly(A) polymerase, cDNA was synthesized with a suitable poly(T) adapter. miR-224 expression was assessed by quantitative real-time PCR and analyzed with the comparative quantification cycle method, Cq(2−ΔΔCq). We performed comprehensive biostatistical analyses to explore the clinical value of miR-224 in prostate cancer. RESULTS miR-224 expression was significantly downregulated in malignant samples compared with benign samples (P < 0.001). Higher miR-224 expression levels were found in prostate tumors that were less aggressive (P = 0.017) and in an earlier disease stage (P = 0.018). Patients with prostate cancer who were positive for miR-224 had significantly enhanced progression-free survival intervals compared with miR-224–negative patients (P = 0.021). Univariate bootstrap Cox regression confirmed that miR-224 was associated with favorable prognosis (hazard ratio 0.314, P = 0.013); nonetheless, multivariate analysis, adjusted for conventional markers, did not identify miR-224 as an independent prognostic indicator. CONCLUSIONS miR-224 is aberrantly expressed in prostate cancer. Its assessment by cost-effective quantitative molecular methodologies could provide a useful biomarker for prostate cancer.

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