Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres

2009 ◽  
pp. n/a-n/a ◽  
Author(s):  
Arnida ◽  
Alexander Malugin ◽  
Hamidreza Ghandehari
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Comparative Study ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Prostate Cancer Cells

scholarly journals Comparative Study of Rapamycin and Temsirolimus Demonstrates Superimposable Anti-Tumour Potency on Prostate Cancer Cells

2012 ◽  
Vol 112 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Paolo Fagone ◽  
Marco Donia ◽  
Katia Mangano ◽  
Cinzia Quattrocchi ◽  
Santa Mammana ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Comparative Study ◽  
Cancer Cells ◽  
Prostate Cancer Cells

Multifunctional targeted therapy system based on99mTc/177Lu-labeled gold nanoparticles-Tat(49-57)-Lys3-bombesin internalized in nuclei of prostate cancer cells

2013 ◽  
Vol 56 (13) ◽  
pp. 663-671 ◽  
Author(s):  
Nallely Jiménez-Mancilla ◽  
Guillermina Ferro-Flores ◽  
Clara Santos-Cuevas ◽  
Blanca Ocampo-García ◽  
Myrna Luna-Gutiérrez ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticles ◽  
Targeted Therapy ◽  
Cancer Cells ◽  
Prostate Cancer Cells

scholarly journals Telomerase Inhibition, Telomere Shortening, and Cellular Uptake of the Perylene Derivatives PM2 and PIPER in Prostate Cancer Cells

2019 ◽  
Vol 42 (6) ◽  
pp. 906-914 ◽  
Author(s):  
Navakoon Kaewtunjai ◽  
Ratasark Summart ◽  
Ariyaphong Wongnoppavich ◽  
Bannakij Lojanapiwat ◽  
T. Randall Lee ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Prostate Cancer Cells ◽  
Telomere Shortening ◽  
Telomerase Inhibition ◽  
Perylene Derivatives

scholarly journals Synthesis, characterization and cellular location of cytotoxic constitutional organometallic isomers of rhenium delivered on a cyanocobalmin scaffold

2015 ◽  
Vol 44 (15) ◽  
pp. 6999-7008 ◽  
Author(s):  
Giuseppe Santoro ◽  
Theodora Zlateva ◽  
Albert Ruggi ◽  
Luca Quaroni ◽  
Fabio Zobi
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Vitamin B ◽  
Prostate Cancer Cells ◽  
Cellular Location ◽  
3T3 Fibroblasts

Constitutional isomers based on vitamin B12 and a fluorescent rhenium diimine complex were prepared, characterized, tested against PC-3 prostate cancer cells and investigated via IR spectromicroscopy for cellular uptake by live 3T3 fibroblasts.

scholarly journals Development of EphA2 siRNA-loaded lipid nanoparticles and combination with a small‐molecule histone demethylase inhibitor in prostate cancer cells and tumor spheroids

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ezgi Oner ◽  
Mustafa Kotmakci ◽  
Anne-Marie Baird ◽  
Steven G. Gray ◽  
Bilge Debelec Butuner ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Transfection Efficiency ◽  
3D Culture ◽  
Lipid Nanoparticles ◽  
In Vivo Studies ◽  
Great Promise ◽  
Prostate Cancer Cells ◽  
Tumor Spheroids

Abstract Background siRNAs hold a great potential for cancer therapy, however, poor stability in body fluids and low cellular uptake limit their use in the clinic. To enhance the bioavailability of siRNAs in tumors, novel, safe, and effective carriers are needed. Results Here, we developed cationic solid lipid nanoparticles (cSLNs) to carry siRNAs targeting EphA2 receptor tyrosine kinase (siEphA2), which is overexpressed in many solid tumors including prostate cancer. Using DDAB cationic lipid instead of DOTMA reduced nanoparticle size and enhanced both cellular uptake and gene silencing in prostate cancer cells. DDAB-cSLN showed better cellular uptake efficiency with similar silencing compared to commercial transfection reagent (Dharmafect 2). After verifying the efficacy of siEphA2-loaded nanoparticles, we further evaluated a potential combination with a histone lysine demethylase inhibitor, JIB-04. Silencing EphA2 by siEphA2-loaded DDAB-cSLN did not affect the viability (2D or 3D culture), migration, nor clonogenicity of PC-3 cells alone. However, upon co-administration with JIB-04, there was a decrease in cellular responses. Furthermore, JIB-04 decreased EphA2 expression, and thus, silencing by siEphA2-loaded nanoparticles was further increased with co-treatment. Conclusions We have successfully developed a novel siRNA-loaded lipid nanoparticle for targeting EphA2. Moreover, preliminary results of the effects of JIB-04, alone and in combination with siEphA2, on prostate cancer cells and prostate cancer tumor spheroids were presented for the first time. Our delivery system provides high transfection efficiency and shows great promise for targeting other genes and cancer types in further in vitro and in vivo studies.

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