Peri‐tumoural stroma collagen organization of invasive ductal carcinoma assessed by polarized light microscopy differs between OncotypeDX risk group

2020 ◽  
Vol 13 (11) ◽  
Author(s):  
Blake Jones ◽  
Georgia Thomas ◽  
Jillian Sprenger ◽  
Sharon Nofech‐Mozes ◽  
Mohammadali Khorasani ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Invasive Ductal Carcinoma ◽  
Ductal Carcinoma ◽  
Risk Group ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Collagen Organization

scholarly journals A polarized light microscopy method for accurate and reliable grading of collagen organization in cartilage repair

2011 ◽  
Vol 19 (1) ◽  
pp. 126-135 ◽  
Author(s):  
A. Changoor ◽  
N. Tran-Khanh ◽  
S. Méthot ◽  
M. Garon ◽  
M.B. Hurtig ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Cartilage Repair ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Collagen Organization ◽  
Microscopy Method

scholarly journals Obesity alters the collagen organization and mechanical properties of murine cartilage

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amber T. Collins ◽  
Guoli Hu ◽  
Hunter Newman ◽  
Michael H. Reinsvold ◽  
Monique R. Goldsmith ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Light Microscopy ◽  
High Fat Diet ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Indentation Modulus ◽  
Knee Cartilage ◽  
High Fat ◽  
Collagen Organization ◽  
Significant Difference

AbstractOsteoarthritis is a debilitating disease characterized by cartilage degradation and altered cartilage mechanical properties. Furthermore, it is well established that obesity is a primary risk factor for osteoarthritis. The purpose of this study was to investigate the influence of obesity on the mechanical properties of murine knee cartilage. Two-month old wild type mice were fed either a normal diet or a high fat diet for 16 weeks. Atomic force microscopy-based nanoindentation was used to quantify the effective indentation modulus of medial femoral condyle cartilage. Osteoarthritis progression was graded using the OARSI system. Additionally, collagen organization was evaluated with picrosirius red staining imaged using polarized light microscopy. Significant differences between diet groups were assessed using t tests with p < 0.05. Following 16 weeks of a high fat diet, no significant differences in OARSI scoring were detected. However, we detected a significant difference in the effective indentation modulus between diet groups. The reduction in cartilage stiffness is likely the result of disrupted collagen organization in the superficial zone, as indicated by altered birefringence on polarized light microscopy. Collectively, these results suggest obesity is associated with changes in knee cartilage mechanical properties, which may be an early indicator of disease progression.

scholarly journals Comparative Assessment of Primary Osteoarthritis Progression Using Conventional Histopathology, Polarized Light Microscopy, and Immunohistochemistry

Cartilage ◽  
2020 ◽  
pp. 194760352093845 ◽  
Author(s):  
V.P. Mantripragada ◽  
W. Gao ◽  
N.S. Piuzzi ◽  
C.D. Hoemann ◽  
G.F. Muschler ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Healthy Adult ◽  
Femoral Condyle ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Current Standard ◽  
Superficial Zone ◽  
Collagen Organization ◽  
Primary Osteoarthritis ◽  
Early Primary

Objective Evaluation of collagen orientation and arrangement in articular cartilage can improve our understanding of primary osteoarthritis (OA) progression and targeted therapies. Our goal was to determine if polarized light microscopy (PLM) for collagen organization is useful in identifying early primary OA features in comparison to current standard histopathological methods. Design Osteochondral specimens from 90 total knee arthroplasty patients with relatively preserved lateral femoral condyle were scored using (1) histological-histochemical grading system (HHGS); (2) Osteoarthritis Research Society International (OARSI); (3) PLM-Changoor system for repair cartilage, scores ranging between 0 (totally disorganized cartilage) and 5 (healthy adult cartilage); and (4) new PLM system for primary OA cartilage with superficial zone PLM (PLM-SZ) and deep zone PLM (PLM-DZ) scores, each ranging between 0 (healthy adult SZ and DZ collagen organization) and 4 (total loss of collagen organization). Serial sections were stained for collagen I and II antibodies. Spearman correlation coefficients ( rs) were determined. Results The associations between: (1) PLM-Changoor and HHGS or OARSI were weak ( rs = −0.36) or moderate ( rs = −0.56); (2) PLM-SZ and HHGS or OARSI were moderate ( rs = 0.46 or rs = 0.53); and (3) PLM-DZ and HHGS or OARSI were poor ( rs = 0.31 or rs = 0.21), respectively. Specimens exhibiting early and mild OA (HHGS < 5 and OARSI < 8.6) had PLM-SZ and PLM-DZ scores between 0 and 4 and between 0 and 3, respectively, and indicated new histopathological features not currently considered by HHGS/OARSI. Conclusions PLM was effective at identifying early SZ and DZ collagen alterations that were not evident in the traditional scoring systems. Incorporating PLM scores and/or additional HHGS/OARSI features can help improve characterization of early primary OA cartilage.

Ferroelastic domains and phase transitions in organic–inorganic hybrid perovskite CH3NH3PbBr3

2021 ◽  
Author(s):  
Maryam Bari ◽  
Alexei A. Bokov ◽  
Zuo-Guang Ye
Keyword(s):  
Phase Transitions ◽  
Electric Field ◽  
Light Microscopy ◽  
Domain Structure ◽  
Polarized Light ◽  
External Stress ◽  
Polarized Light Microscopy ◽  
Inorganic Hybrid ◽  
Hybrid Perovskite ◽  
Ferroelastic Domains

Polarized light microscopy reveals twin domains and symmetry of the phases in CH3NH3PbBr3 crystal; domain structure remains unresponsive to electric field but changes under external stress, confirming ferroelasticity while ruling out ferroelectricity.

scholarly journals Exploiting the Binocular Head in Polarized Light Microscopy

1994 ◽  
Vol 2 (4) ◽  
pp. 16-16
Author(s):  
Walter C. McCrone
Keyword(s):  
Light Microscopy ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
The Other ◽  
Monocular Viewing ◽  
Area Of Interest

Having been brought up on monocular microscopes I find the omnipresent binocular systems a luxury. To support this viewpoint I'd like to suggest some benefits you may not have considered.Because I'm used to monocular viewing I sometimes use two different oculars, say 10X and 25X, in order to scan quickly to find an area of interest and then to examine the detail with higher magnification. Occasionally I use both oculars simultaneously and “concentrate” on either image to the exclusion of the other. A better way is to set the interocular distance at the extreme setting most different from your own interocular distance. By moving your head about a centimeter either way you can use either ocular.

Structural Morphology of Rabbit Patella and Suprapatella Cartilage by Microscopic MRI and Polarized Light Microscopy

Cartilage ◽  
2021 ◽  
pp. 194760352199088
Author(s):  
Hannah Mantebea ◽  
Syeda Batool ◽  
Mouhamad Hammami ◽  
Yang Xia
Keyword(s):  
Articular Cartilage ◽  
Light Microscopy ◽  
Design Method ◽  
Strong Dependence ◽  
Polarized Light ◽  
Polarized Light Microscopy ◽  
Structural Morphology ◽  
Pixel Resolution ◽  
Patellar Cartilage ◽  
Quantitative Results

Objective In order to appreciate the roles articular cartilage of sesamoid bones and sesamoid fibrocartilage play in anatomy and pathology, the articular cartilage of the patella ( n = 4) and suprapatella ( n = 4) (a sesamoid fibrocartilage) of 12 to 14 weeks old New Zealand rabbits were studied qualitatively and quantitatively. Design/Method The intact knee joints and block specimens from the joints were imaged using microscopic magnetic resonance imaging (µMRI) at a 97.6-µm pixel resolution for the former and 19.5-µm resolution for the latter. Histological sections were made out of the µMRI-imaged specimens, which were imaged using polarized light microscopy (PLM) at 0.25-, 1-, and 4-µm pixel resolutions. Results The patella cartilage varied in thickness across the medial to lateral ends of the sesamoid bone with the central medial aspect slightly thicker than the lateral aspect. The suprapatella fibrocartilage decreased proximally away from the knee joint. Quantitative results of patellar cartilage showed strong dependence of fiber orientation with the tissue depth. Three histological zones can be clearly observed, which are similar to articular cartilage from other large animals. The sesamoid fibrocartilage has one thin surface layer (10 µm thick) of parallel-arranged structured fibers followed immediately by the majority of random fibers in bulk tissue. T2 relaxation time anisotropy was observed in the patellar cartilage but not in the bulk fibrocartilage. Conclusion Given the different functions of these 2 different types of cartilages in joint motion, these quantitative results will be beneficial to future studies of joint diseases using rabbits as the animal model.

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