Polylactic acid–phosphate glass composite foams as scaffolds for bone tissue engineering

Correction for ‘Development of Fe3O4 integrated polymer/phosphate glass composite scaffolds for bone tissue engineering’ by Raji Govindan et al., Mater. Adv., 2020, DOI: 10.1039/d0ma00525h.

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Additive-Manufactured Gyroid Scaffolds of Magnesium Oxide, Phosphate Glass Fiber and Polylactic Acid Composite for Bone Tissue Engineering

Polymers ◽

10.3390/polym13020270 ◽

2021 ◽

Vol 13 (2) ◽

pp. 270

Author(s):

Lizhe He ◽

Xiaoling Liu ◽

Chris Rudd

Keyword(s):

Tissue Engineering ◽

Glass Fiber ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Polylactic Acid ◽

Phosphate Glass ◽

Bone Repair ◽

Composite Scaffold ◽

Aqueous Environment ◽

Neutralizing Agent

Composites of biodegradable phosphate glass fiber and polylactic acid (PGF/PLA) show potential for bone tissue engineering scaffolds, due to their ability to release Ca, P, and Mg during degradation, thus promoting the bone repair. Nevertheless, glass degradation tends to acidify the surrounding aqueous environment, which may adversely affect the viability and bone-forming activities of osteoblasts. In this work, MgO was investigated as a neutralizing agent. Porous network-phase gyroid scaffolds were additive-manufactured using four different materials: PLA, MgO/PLA, PGF/PLA, and (MgO + PGF)/PLA. The addition of PGF enhanced compressive properties of scaffolds, and the resultant scaffolds were comparably strong and stiff with human trabecular bone. While the degradation of PGF/PLA composite induced considerable acidity in degradation media and intensified the degradation of PGF in return, the degradation media of (MgO + PGF)/PLA maintained a neutral pH close to a physiological environment. The experiment results indicated the possible mechanism of MgO as the neutralizing agent: the local acidity was buffered as the MgO reacted with the acidic degradation products thereby inhibiting the degradation of PGF from being intensified in an acidic environment. The (MgO + PGF)/PLA composite scaffold appears to be a candidate for bone tissue engineering.

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Poly(d,l-Lactic acid) Composite Foams Containing Phosphate Glass Particles Produced via Solid-State Foaming Using CO2 for Bone Tissue Engineering Applications

Polymers ◽

10.3390/polym12010231 ◽

2020 ◽

Vol 12 (1) ◽

pp. 231 ◽

Cited By ~ 1

Author(s):

Maziar Shah Mohammadi ◽

Ehsan Rezabeigi ◽

Jason Bertram ◽

Benedetto Marelli ◽

Richard Gendron ◽

...

Keyword(s):

Tissue Engineering ◽

Lactic Acid ◽

Solid State ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Melt Compounding ◽

Composite Foams ◽

Biodegradable Poly ◽

Gaseous Carbon Dioxide ◽

Highly Porous

This study reports on the production and characterization of highly porous (up to 91%) composite foams for potential bone tissue engineering (BTE) applications. A calcium phosphate-based glass particulate (PGP) filler of the formulation 50P2O5-40CaO-10TiO2 mol.%, was incorporated into biodegradable poly(d,l-lactic acid) (PDLLA) at 5, 10, 20, and 30 vol.%. The composites were fabricated by melt compounding (extrusion) and compression molding, and converted into porous structures through solid-state foaming (SSF) using high-pressure gaseous carbon dioxide. The morphological and mechanical properties of neat PDLLA and composites in both nonporous and porous states were examined. Scanning electron microscopy micrographs showed that the PGPs were well dispersed throughout the matrices. The highly porous composite systems exhibited improved compressive strength and Young’s modulus (up to >2-fold) and well-interconnected macropores (up to ~78% open pores at 30 vol.% PGP) compared to those of the neat PDLLA foam. The pore size of the composite foams decreased with increasing PGPs content from an average of 920 µm for neat PDLLA foam to 190 µm for PDLLA-30PGP. Furthermore, the experimental data was in line with the Gibson and Ashby model, and effective microstructural changes were confirmed to occur upon 30 vol.% PGP incorporation. Interestingly, the SSF technique allowed for a high incorporation of bioactive particles (up to 30 vol.%—equivalent to ~46 wt.%) while maintaining the morphological and mechanical criteria required for BTE scaffolds. Based on the results, the SSF technique can offer more advantages and flexibility for designing composite foams with tunable characteristics compared to other methods used for the fabrication of BTE scaffolds.

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Titanium phosphate glass microspheres for bone tissue engineering

Acta Biomaterialia ◽

10.1016/j.actbio.2012.07.023 ◽

2012 ◽

Vol 8 (11) ◽

pp. 4181-4190 ◽

Cited By ~ 45

Author(s):

Nilay J. Lakhkar ◽

Jeong-Hui Park ◽

Nicola J. Mordan ◽

Vehid Salih ◽

Ivan B. Wall ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Phosphate Glass ◽

Titanium Phosphate ◽

Glass Microspheres

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One-pot synthesis of macro-mesoporous bioactive glasses/polylactic acid for bone tissue engineering

Materials Science and Engineering C ◽

10.1016/j.msec.2014.07.042 ◽

2014 ◽

Vol 43 ◽

pp. 367-374 ◽

Cited By ~ 24

Author(s):

Xiao Han ◽

Dan Wang ◽

Xiang Chen ◽

Huiming Lin ◽

Fengyu Qu

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Polylactic Acid ◽

Bioactive Glasses ◽

One Pot ◽

One Pot Synthesis

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Production, by co-grinding in a media mill, of porous biodegradable polylactic acid–apatite composite materials for bone tissue engineering

Powder Technology ◽

10.1016/j.powtec.2008.04.043 ◽

2009 ◽

Vol 190 (1-2) ◽

pp. 89-94 ◽

Cited By ~ 16

Author(s):

Nadine Le Bolay ◽

Véronique Santran ◽

Gérard Dechambre ◽

Christèle Combes ◽

Christophe Drouet ◽

...

Keyword(s):

Tissue Engineering ◽

Composite Materials ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Polylactic Acid

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Three-dimensional Printed Polylactic Acid and Hydroxyapatite Composite Scaffold with Urine-derived Stem Cells Treatment for Bone Defects

10.21203/rs.3.rs-996295/v1 ◽

2021 ◽

Author(s):

Xiang Zhang ◽

Jialei Chen ◽

Hongren Wang ◽

Xin Duan ◽

Feng Gao

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Polylactic Acid ◽

Composite Scaffold ◽

Bone Defects ◽

Skull Defect ◽

Defect Area ◽

3D Printed

Abstract BACKGROUND: Bone defects still pose various challenges in osteology. As one of the treatment options for bone defects, bone tissue engineering requires biomaterials with good biocompatibility and seed cells with good differentiation capacity. This study aimed to fabricate a 3D-printed polylactic acid and hydroxyapatite (PLA/HA) composite scaffold with urine-derived stem cells (USCs) to study its therapeutic effect in a model of skull defect in rats.METHODS: USCs, isolated and extracted from the urine of healthy adult males, were inoculated onto a 3D-printed PLA/HA composite scaffold and a PLA scaffold. Skull defect model rats were randomly divided into three groups (control, PLA, and PLA/HA). Twelve weeks after implanting scaffolds containing USCs into rats with a skull defect, the therapeutic efficacy was evaluated by real-time PCR, micro-CT, histology, and immunohistochemistry.RESULTS: The 3D-printed PLA/HA composite scaffold had good mechanical properties and porosity. The adhesion and proliferation of USCs on scaffolds also demonstrated excellent biocompatibility. PLA and PLA/HA containing USCs promoted bone regeneration in the defect area, supported by the general observation and CT images at 12 weeks after treatment, with coverage of 74.6%±1.9% and 96.7%±1.6%, respectively. Immunohistochemical staining showed a progressive process of new bone formation on PLA/HA scaffolds containing USCs at the defect site compared to that in PLA and control groups.CONCLUSION: The 3D-printed PLA/HA composite scaffold with USCs was successfully applied to the skull defect in rats. Under the linkage of the scaffold, the proliferation, differentiation, and osteogenesis expression of USCs were promoted near the bone defect area. These findings demonstrated broad application prospects of PLA/HA scaffolds with USCs in bone tissue engineering.

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