Degree of biological apatitec-axis orientation rather than bone mineral density controls mechanical function in bone regenerated using recombinant bone morphogenetic protein-2

Influence of bone morphogenetic protein (BMP-2) in its local application in combination with demineralized lyophilized bone implant (DLBI) upon the process of osteogenesis and bone mineral density in the zone of surgical intervention and the whole segment (tibia) was studied in experiment (40 rats). The animals were divided into 2 groups. In animals from the study group the defect zone was filled with both DLBI and BMP-2 while in control group only with DLBI. In evaluation of morphologic changes by points at terms 7 and 12 weeks no reliable differences between the groups were noted. However at 12 weeks in study group cases of marked osteogenesis with full remodeling of bone implant were observed. It was shown that use of BMP-2 in combination with DLBI reliably increased bone mineral density both in the zone of surgical intervention and in the segment as a whole. That fact was regarded as the evidence of BMP-2 positive effect upon the mechanical strength of the forming regenerate.

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Tooth Eruption into the Newly Generated Bone Induced by Recombinant Human Bone Morphogenetic Protein-2

The Cleft Palate-Craniofacial Journal ◽

10.1597/1545-1569_2002_039_0449_teitng_2.0.co_2 ◽

2002 ◽

Vol 39 (4) ◽

pp. 449-456 ◽

Cited By ~ 1

Author(s):

Atsushi Kitamura ◽

Nobuyoshi Motohashi ◽

Tatsuo Kawamoto ◽

Yoshiyuki Baba ◽

Shoichi Suzuki ◽

...

Keyword(s):

Bone Morphogenetic Protein ◽

Alveolar Bone ◽

Treated Group ◽

Tooth Eruption ◽

Human Bone ◽

Bone Morphogenetic Protein 2 ◽

Mineral Density ◽

Morphogenetic Protein ◽

Human Bone Morphogenetic Protein ◽

Recombinant Human Bone

Objective The purpose of this study was to observe tooth eruption pattern into the newly generated bone induced by recombinant human bone morphogenetic protein-2 (rhBMP-2). Method Three 12-week-old beagle dogs were used. Following wound healing of the extracted maxillary third deciduous incisor at the age of 13 weeks, a resection of maxillary alveolar bone (8 mm by 8 mm by 8 mm) was carried out, accompanied by extraction of the maxillary second incisor tooth germ. The rhBMP-2 (5 μg/100 μL or 10 μg/100 μL) was implanted in the resected area. A group that was not implanted after resection and a nonresected group were used as controls. Serial changes of the eruption pattern of the maxillary third incisor in the implanted area were observed radiographically. Oxytetracycline and calcein were employed as bone markers. Nine weeks after the implantation, the animals were sacrificed. The samples were observed histologically and analyzed using peripheral quantitative computerized tomography to acquire the bone mineral density (BMD). Results In the rhBMP-2 (5 μg/100 μL)-treated group, histological findings and the BMD of the newly generated bone were almost same as in the non-resected group. The maxillary third incisor erupted through the implanted area. However, in the rhBMP-2 (10 μg/100 μL)-treated group, more osteoinductive activity and significantly higher BMD were observed in the newly generated bone. The maxillary third incisor erupted mesially so as to avoid this implanted area. Conclusion Results showed that bone remodeling and tooth eruption through the newly generated bone appear to be dose dependent. Accordingly, proper dosage of rhBMP-2 should be determined for successful clinical application.

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Core Ossification of Bone Morphogenetic Protein-2-Loaded Collagenated Bone Mineral in the Sinus

Tissue Engineering Part A ◽

10.1089/ten.tea.2020.0151 ◽

2020 ◽

Author(s):

Jae-Kook Cha ◽

Young Woo Song ◽

Sungtae Kim ◽

Daniel S. Thoma ◽

Ui-Won Jung ◽

...

Keyword(s):

Bone Morphogenetic Protein ◽

Bone Mineral ◽

Bone Morphogenetic Protein 2 ◽

Morphogenetic Protein

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Nephroblastoma Overexpressed (Nov) Inactivation Sensitizes Osteoblasts to Bone Morphogenetic Protein-2, But Nov Is Dispensable for Skeletal Homeostasis

Endocrinology ◽

10.1210/en.2009-0574 ◽

2010 ◽

Vol 151 (1) ◽

pp. 221-233 ◽

Cited By ~ 24

Author(s):

Ernesto Canalis ◽

Anna Smerdel-Ramoya ◽

Deena Durant ◽

Aris N. Economides ◽

Wesley G. Beamer ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Bone Morphogenetic Protein ◽

Tissue Growth ◽

Bone Morphogenetic Protein 2 ◽

Wild Type ◽

Reporter Construct ◽

Mineral Density ◽

Morphogenetic Protein ◽

Skeletal Homeostasis ◽

Trabecular Number

Abstract Overexpression of nephroblastoma overexpressed (Nov), a member of the Cyr 61, connective tissue growth factor, Nov family of proteins, inhibits osteoblastogenesis and causes osteopenia. The consequences of Nov inactivation on osteoblastogenesis and the postnatal skeleton are not known. To study the function of Nov, we inactivated Nov by homologous recombination. Nov null mice were maintained in a C57BL/6 genetic background after the removal of the neomycin selection cassette and compared with wild-type controls of identical genetic composition. Nov null mice were identified by genotyping and absent Nov mRNA in calvarial extracts and osteoblast cultures. Nov null mice did not exhibit developmental skeletal abnormalities or postnatal changes in weight, femoral length, body fat, or bone mineral density and appeared normal. Bone volume and trabecular number were decreased only in 1-month-old female mice. In older mice, after 7 months of age, osteoblast surface and bone formation were increased in females, and osteoclast and eroded surfaces were increased in male Nov null mice. Calvarial osteoblasts from Nov null mice displayed enhanced alkaline phosphatase activity, alkaline phosphatase mRNA, and transactivation of a bone morphogenetic protein (BMP)/phosphorylated mothers against decapentaplegic reporter construct in response to BMP-2. Similar results were obtained after the down-regulation of Nov by RNA interference in ST-2 stromal and MC3T3 cells. Osteoclast number was increased in marrow stromal cell cultures from Nov null mice. Surface plasmon resonance demonstrated direct interactions between Nov and BMP-2. In conclusion, Nov sensitizes osteoblasts to BMP-2, but Nov is dispensable for the maintenance of bone mass.

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