Effects of epidermal growth factor on parathyroid hormone-related protein production by mammary epithelial cells

2009 ◽  
Vol 9 (5) ◽  
pp. 639-644 ◽  
Author(s):  
S.L. Ferrari ◽  
R. Rizzoli ◽  
J.-P. Bonjour
Keyword(s):  
Parathyroid Hormone ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Protein Production ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein ◽  
Epidermal Growth

Parathyroid hormone-related protein increases cAMP production in mammary epithelial cells

1993 ◽  
Vol 264 (3) ◽  
pp. E471-E475 ◽  
Author(s):  
S. Ferrari ◽  
R. Rizzoli ◽  
C. Chaponnier ◽  
G. Gabbiani ◽  
J. P. Bonjour
Keyword(s):  
Parathyroid Hormone ◽  
Epithelial Cells ◽  
Breast Tissue ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Immunofluorescent Staining ◽  
Regulatory Function ◽  
Related Protein ◽  
Camp Production ◽  
Parathyroid Hormone Related Protein

Parathyroid hormone-related protein (PTHrP) is a major cause of malignant hypercalcemia but has been found in many nontumoral tissues as well. Thus it is produced by the mammary gland during lactation and released into milk. Whether PTHrP directly affects breast tissue is however not known. We investigated the effects of PTHrP on adenosine 3',5'-cyclic monophosphate (cAMP) production in primary cultures of mammary epithelial cells isolated from lactating rats. On the 7th day in culture, synthetic PTHrP-(1-34), recombinant (r) PTHrP-(1-108), and rPTHrP-(1-141) stimulated cAMP production in a concentration-dependent manner. Thus PTHrP-(1-34) induced a 1.92 +/- 0.04-fold stimulation of cAMP production (mean +/- SE, n = 5 separate experiments, P < 0.001). At the time of maximal responsiveness to PTHrP, a significant proportion of the cells was characterized by an elongated shape and a positive immunofluorescent staining for both prekeratin and alpha-smooth muscle actin 1, compatible with a myoepithelial phenotype. It therefore appears that PTHrP can stimulate the production of cAMP in mammary cells, suggesting a possible autocrine/paracrine regulatory function for PTHrP in breast tissue.

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