Prolonged culture of HOS 58 human osteosarcoma cells with 1,25-(OH)2-D3, TGF-beta, and dexamethasone reveals physiological regulation of alkaline phosphatase, dissociated osteocalcin gene expression, and protein synthesis and lack of mineralization

2002 ◽  
Vol 85 (2) ◽  
pp. 279-294 ◽  
Author(s):  
Heide Siggelkow ◽  
Michael Schenck ◽  
Manfred Rohde ◽  
Volker Viereck ◽  
Simone Tauber ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Protein Synthesis ◽  
Tgf Beta ◽  
Osteosarcoma Cells ◽  
Physiological Regulation ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Osteocalcin Gene ◽  
Prolonged Culture

scholarly journals Functional Interference Between AP-1 and the Vitamin D Receptor on Osteocalcin Gene Expression in Human Osteosarcoma Cells

1994 ◽  
Vol 224 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Tiina Jaaskelainen ◽  
Asta Pirskanen ◽  
Sanna Ryhanen ◽  
Jorma J. Palvimo ◽  
Hector F. Deluca ◽  
...  
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Functional Interference ◽  
Human Osteosarcoma Cells ◽  
Osteocalcin Gene

Osteocalcin gene methylation in different lines of human osteosarcoma cells

1992 ◽  
Vol 17 ◽  
pp. 201
Author(s):  
S. Iääskeläinen ◽  
A Pirskanen ◽  
P.H. Mäenpää
Keyword(s):  
Osteosarcoma Cells ◽  
Gene Methylation ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Osteocalcin Gene

scholarly journals Presence and activity of alkaline phosphatase in two human osteosarcoma cell lines.

1989 ◽  
Vol 37 (7) ◽  
pp. 1069-1074 ◽  
Author(s):  
J C Randall ◽  
D C Morris ◽  
S Zeiger ◽  
K Masuhara ◽  
T Tsuda ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Membrane ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Osteogenic Sarcoma ◽  
Ultrastructural Level ◽  
Osteosarcoma Cell ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

The presence and activity of alkaline phosphatase in SAOS-2 and TE-85 human osteosarcoma cells grown in culture were examined at the ultrastructural level. A monoclonal antibody raised against purified human bone osteosarcoma alkaline phosphatase was used to localize the enzyme in cultures of the osteosarcoma cells. Similar cultures were analyzed for alkaline phosphatase activity using an enzyme cytochemical method with cerium as the capture agent. Alkaline phosphatase was immunolocalized at the light microscopic level in an osteogenic sarcoma and ultrastructurally on the SAOS-2 cell membrane and the enclosing membrane of extracellular vesicular structures close to the cells. In contrast, the TE-85 cells were characterized by the absence of all but a few traces of immunolabeling at the cell surface. Enzyme cytochemical studies revealed strong alkaline phosphatase activity on the outer surface of the SAOS-2 cell membrane. Much lower enzyme activity was observed in the TE-85 cells. The results support biochemical data from previous studies and confirm that SAOS-2 cells have a significantly greater concentration of alkaline phosphatase at the plasma membrane.

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