Polyamide Thin-Layer Chromatography. III. A Modified Method of Preparation of Thin-Layer and its Application to some Naturally Occurring Phenolic Substances

1963 ◽  
Vol 10 (2-3) ◽  
pp. 146-155 ◽  
Author(s):  
Kung-Tsung Wang ◽  
Yau-Tang Lin
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Modified Method ◽  
Naturally Occurring ◽  
Phenolic Substances ◽  
Layer Chromatography

scholarly journals The use of Bacillus subtilis for screening fusaric acid production by Fusarium spp.

2009 ◽  
Vol 27 (No. 3) ◽  
pp. 203-209 ◽  
Author(s):  
A. Šrobárová ◽  
Š. Eged ◽  
J. Teixeira Da Silva ◽  
A. Ritieni ◽  
A. Santini
Keyword(s):  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Thin Layer ◽  
Fusarium Oxysporum ◽  
Thin Layer Chromatography ◽  
Screening Test ◽  
Acid Production ◽  
Fusaric Acid ◽  
Modified Method ◽  
Layer Chromatography

Fusaric acid (FA) is one of the most important secondary metabolites produced by <I>Fusarium oxysporum</I> (Schlecht) (FO), <I>F. solani</I> (Mart.) Appel & Wollenweber, and <I>F. moniliforme</I> Sheldon. It is toxic to humans, many plants, and microorganisms and it enhances the toxicity of fumonisin and trichothecene. A simple and rapid method for fusaric acid (FA) screening in <I>Fusarium</I> isolates was developed. In this study, several strains of <I>Fusarium oxysporum</I> were tested for their ability to produce FA by using a suitable race of <I>Bacillus subtilis</I> as the bioassay. A modified method using small agar blocks with the fungus producing FA was applied in the screening test. FA standard and <I>F. culmorum</I> were used as controls. The experimental <I>F. oxysporum</I> isolates and FA standard produced transparent zones on the plates with <I>Bacillus subtilis</I>. The differences in size of the transparent zones corresponded to the quantity of FA when thin-layer chromatography was used.

Screening rapeseed (B. napus) for low glucosinolate levels

1975 ◽  
Vol 15 (75) ◽  
pp. 561
Author(s):  
CJ Rayner ◽  
JP Sang ◽  
CG Buzza
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Preparative Thin Layer Chromatography ◽  
Large Majority ◽  
Breeding Programme ◽  
Initial Screening ◽  
Modified Method ◽  
Chemical Techniques ◽  
Layer Chromatography ◽  
Quantitative Chemical

As part of a rapeseed breeding programme crosses were made between the low glucosinolate cultivar Bronowski and high glucosinolate cultivars Oro, Zephyr, Argentine, Chisaya and Norin 16. The 2887 F2 lines resulting from these crosses were initially screened for low glucosinolate character by the glukatest. Lines passing this test were examined in more detail using quantitative chemical techniques. The efficiency of the glukatest as an initial screening technique was demonstrated by the fact that it eliminated the large majority of high glucosinolate lines. L-5-vinyl-2-oxazolidinethione (OZT) was isolated from rapeseed and purified by a modified method using preparative thin layer chromatography. This method is described in detail.

Practical Application of Thin-Layer Chromatography for Detection of Polyphosphates in Seafood

1995 ◽  
Vol 78 (5) ◽  
pp. 1328-1332 ◽  
Author(s):  
Judith Krzynowek ◽  
Laurie J Panunzio
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Sampling Techniques ◽  
Practical Application ◽  
Naturally Occurring ◽  
Fresh Products ◽  
Layer Chromatography

Abstract The practical application of previously published thin-layer chromatographic (TLC) methods to polyphosphate detection in seafood was determined. Exogenous polyphosphates are distinguishable from naturally occurring phosphates by using TLC; their detection can be enhanced by sampling techniques. Polyphosphates will eventually hydrolyze to monophosphates and make detection virtually impossible. Hydrolysis and therefore, nondetection, is accelerated by certain commercial practices. Results indicate that TLC detection of added phosphates can be used as an inspection tool for fresh products and for frozen products stored about 1 year.

Determination of Canthaxanthin in Concentrates and Feeds

1972 ◽  
Vol 55 (1) ◽  
pp. 110-113
Author(s):  
M Osadca ◽  
M Araujo ◽  
E De Ritter
Keyword(s):  
Aqueous Solution ◽  
Organic Solvent ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Final Solution ◽  
Naturally Occurring ◽  
Layer Chromatography

Abstract Methods are presented for determining canthaxanthin as a 10% concentrate in beadlet form as such and in feed premixes and finished feeds. Canthaxanthin is liberated from the beadlets by hot aqueous solution and extracted into an organic solvent. In the case of feeds, separation from naturally occurring carotenoids in the extract is achieved by successive column and thin layer chromatography. Canthaxanthin in the final solution is isomerized to equilibrium with iodine and measured spectrophotometrically at 480±2 nm.

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