scholarly journals Rapid, sensitive detection of bacteria in platelet samples with Fountain Flow Cytometry

2017 ◽  
Vol 31 (6) ◽  
pp. e22115 ◽  
Author(s):  
Paul Johnson ◽  
Mika Moriwaki ◽  
Joseph Johnson
Keyword(s):  
Flow Cytometry ◽  
Sensitive Detection ◽  
Fountain Flow

Rapid and highly sensitive detection of Flavobacterium psychrophilum using high gradient immunomagnetic separation with flow cytometry

Aquaculture ◽  
2010 ◽  
Vol 309 (1-4) ◽  
pp. 125-130 ◽  
Author(s):  
Uni Ryumae ◽  
Kyoko Hibi ◽  
Yasutoshi Yoshiura ◽  
Huifeng Ren ◽  
Hideaki Endo
Keyword(s):  
Flow Cytometry ◽  
Immunomagnetic Separation ◽  
Sensitive Detection ◽  
High Gradient ◽  
Flavobacterium Psychrophilum ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

scholarly journals A selective cytotoxic adenovirus vector for concentration of pluripotent stem cells in human pluripotent stem cell-derived neural progenitor cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takamasa Hirai ◽  
Ken Kono ◽  
Rumi Sawada ◽  
Takuya Kuroda ◽  
Satoshi Yasuda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Flow Cytometry ◽  
Stem Cell ◽  
Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Sensitive Detection ◽  
Quality And Safety

AbstractHighly sensitive detection of residual undifferentiated pluripotent stem cells is essential for the quality and safety of cell-processed therapeutic products derived from human induced pluripotent stem cells (hiPSCs). We previously reported the generation of an adenovirus (Ad) vector and adeno-associated virus vectors that possess a suicide gene, inducible Caspase 9 (iCasp9), which makes it possible to sensitively detect undifferentiated hiPSCs in cultures of hiPSC-derived cardiomyocytes. In this study, we investigated whether these vectors also allow for detection of undifferentiated hiPSCs in preparations of hiPSC-derived neural progenitor cells (hiPSC-NPCs), which have been expected to treat neurological disorders. To detect undifferentiated hiPSCs, the expression of pluripotent stem cell markers was determined by immunostaining and flow cytometry. Using immortalized NPCs as a model, the Ad vector was identified to be the most efficient among the vectors tested in detecting undifferentiated hiPSCs. Moreover, we found that the Ad vector killed most hiPSC-NPCs in an iCasp9-dependent manner, enabling flow cytometry to detect undifferentiated hiPSCs intermingled at a lower concentration (0.002%) than reported previously (0.1%). These data indicate that the Ad vector selectively eliminates hiPSC-NPCs, thus allowing for sensitive detection of hiPSCs. This cytotoxic viral vector could contribute to ensuring the quality and safety of hiPSCs-NPCs for therapeutic use.

Fountain Flow Cytometry

2012 ◽  
Author(s):  
Paul Johnson
Keyword(s):  
Flow Cytometry ◽  
Fountain Flow

Flow cytometry-based phagocytosis assay for sensitive detection of opsonic activity of pneumococcal capsular polysaccharide antibodies in human sera

2001 ◽  
Vol 252 (1-2) ◽  
pp. 33-44 ◽  
Author(s):  
Marı́a Eugenia Rodrı́guez ◽  
W.-Ludo Van der Pol ◽  
Jan G.J Van de Winkel
Keyword(s):  
Flow Cytometry ◽  
Capsular Polysaccharide ◽  
Sensitive Detection ◽  
Phagocytosis Assay ◽  
Opsonic Activity ◽  
Human Sera

scholarly journals Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry

10.3791/53205 ◽  
2015 ◽  
Author(s):  
Jennifer L. Furman ◽  
Brandon B. Holmes ◽  
Marc I. Diamond
Keyword(s):  
Flow Cytometry ◽  
Sensitive Detection

The Rapid and Sensitive Detection of Perturbations in Spermatogenesis: Assessment by Quantitative Dual Parameter (DNA/RNA) Flow Cytometry

1989 ◽  
Vol 8 (3) ◽  
pp. 507-523 ◽  
Author(s):  
Jules R. Selden ◽  
Richard T. Robertson ◽  
Judith E. Miller ◽  
Chris Vetter ◽  
David H. Minsker ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Germ Cells ◽  
Biomedical Research ◽  
State Of The Art ◽  
The State ◽  
Sensitive Detection ◽  
Sprague Dawley ◽  
Adult Rats ◽  
Single Bolus ◽  
Preliminary Study

This presentation will concentrate on the emerging field of flow cytometry. The first portion will be a review of the state-of-the-art applications of flow cytometry in the field of biomedical research; the second portion will describe the results of a preliminary study using a published technique that is useful in detecting cellular perturbations in germ cells. The model used in this study was the testis from the Sprague-Dawley rat. Adult rats received a single bolus of busulfan, and their testes were examined up to 56 days postadministration.

Sensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry

2010 ◽  
Vol 138 (1-2) ◽  
pp. 154-158 ◽  
Author(s):  
Wilhelm Gerner ◽  
Maria Stadler ◽  
Sabine E. Hammer ◽  
Daniela Klein ◽  
Armin Saalmüller
Keyword(s):  
Flow Cytometry ◽  
Foxp3 Expression ◽  
Sensitive Detection ◽  
Bovine Lymphocytes

scholarly journals Sensitive detection of RNAs in single cells by flow cytometry

1992 ◽  
Vol 20 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Hong Yu ◽  
Lauren Ernst ◽  
Marc Wagner ◽  
Alan Waggoner
Keyword(s):  
Flow Cytometry ◽  
Single Cells ◽  
Sensitive Detection

Method validation of a flow cytometry assay for sensitive detection of CD20 CAR T cells in peripheral blood

Cytotherapy ◽  
2018 ◽  
Vol 20 (5) ◽  
pp. S60
Author(s):  
S. Biedermann ◽  
A. Kurenbach ◽  
T. Altmann ◽  
C. Siewert ◽  
C. Dose
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Method Validation ◽  
Peripheral Blood ◽  
Sensitive Detection ◽  
Flow Cytometry Assay ◽  
Car T Cells ◽  
Car T
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