scholarly journals Establishment of the reference intervals of whole blood neutrophil phagocytosis by flow cytometry

2021 ◽  
Author(s):  
Fangfang Yang ◽  
Fujie Zhang ◽  
Liping Yang ◽  
Haoran Li ◽  
Yonglie Zhou
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Reference Intervals ◽  
Blood Neutrophil

A Simple and Rapid Method to Determine GPIIb/IIIa-Receptor Inhibitor Activity in Whole Blood

1999 ◽  
Vol 19 (03) ◽  
pp. 134-138
Author(s):  
Gitta Kühnel ◽  
A. C. Matzdorff
Keyword(s):  
Flow Cytometry ◽  
Platelet Count ◽  
Blood Sample ◽  
Platelet Activation ◽  
Whole Blood ◽  
Receptor Occupancy ◽  
Aggregate Formation ◽  
Inhibitor Activity ◽  
Receptor Inhibitor

SummaryWe studied the effect of GPIIb/IIIa-inhibitors on platelet activation with flow cytometry in vitro. Citrated whole blood was incubated with increasing concentrations of three different GPIIb/IIIa-inhibitors (c7E3, DMP728, XJ757), then thrombin or ADP were added and after 1 min the sample was fixed. Samples without c7E3 but with 0.1 U/ml thrombin had a decrease in platelet count. Samples with increasing concentrations of c7E3 had a lesser or no decrease in platelet count. The two other inhibitors (DMP 725, XJ757) gave similar results. GPIIb/IIIa-inhibitors prevent aggregate formation and more single platelets remain in the blood sample. The agonist-induced decrease in platelet count correlates closely with the concentration of the GPIIb/IIIa inhibitor and receptor occupancy. This correlation may be used as a simple measure for inhibitor activity in whole blood.

scholarly journals An easy and reliable whole blood freezing method for flow cytometry immuno‐phenotyping and functional analyses

2021 ◽  
Author(s):  
Cecile Braudeau ◽  
Nina Salabert‐Le Guen ◽  
Justine Chevreuil ◽  
Marie Rimbert ◽  
Jerome C. Martin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Freezing Method ◽  
Functional Analyses

scholarly journals Clinical immunology Whole blood neutrophil chemiluminescence in children with diabetes depending on their clinical condition

2012 ◽  
Vol 4 ◽  
pp. 345-349 ◽  
Author(s):  
Magda Kaczmarek ◽  
Aleksandra Lewandowicz-Uszyńska ◽  
Zdzisława Iwanicka ◽  
Adam Jankowski
Keyword(s):  
Whole Blood ◽  
Clinical Condition ◽  
Clinical Immunology ◽  
Blood Neutrophil

The Effects of Heparin, Protamine, and Heparinase 1 on Platelets in vitro Using Whole Blood Flow Cytometry

2000 ◽  
Vol 9 (4) ◽  
pp. 808-812 ◽  
Author(s):  
Sibylle A. Kozek-Langenecker ◽  
S. Fazal Mohammad ◽  
Takahisa Masaki ◽  
Craig Kamerath ◽  
Alfred K. Cheung
Keyword(s):  
Flow Cytometry ◽  
Blood Flow ◽  
Whole Blood

scholarly journals Photoinstability of S-Nitrosothiols during Sampling of Whole Blood: A Likely Source of Error and Variability in S-Nitrosothiol Measurements

2008 ◽  
Vol 54 (5) ◽  
pp. 916-918 ◽  
Author(s):  
Yiduo Wu ◽  
Fenghua Zhang ◽  
Yang Wang ◽  
Maheshkumaar Krishnamoorthy ◽  
Prabir Roy-Chaudhury ◽  
...  
Keyword(s):  
Whole Blood ◽  
Light Exposure ◽  
Standard Addition ◽  
Aluminum Foil ◽  
Reference Intervals ◽  
Blood Samples ◽  
Butterfly Needle ◽  
Light Protection ◽  
Initial Sampling ◽  
Tube Assembly

Abstract Background: The determination of reference intervals for the concentration of total S-nitrosothiols (RSNOs) in blood is a highly controversial topic, likely because of the inherent instability of these species. Most currently available techniques to quantify RSNOs in blood require considerable sample handling and multiple pretreatment steps during which light exposure is difficult to completely eliminate. We investigated the effect of brief light exposure on the stability of RSNO species in blood during the initial sampling process. Methods: A novel amperometric RSNO sensor, based on an immobilized organoselenium catalyst at the distal tip of an electrochemical nitric oxide detector, was used to determine RSNO species in diluted whole blood without centrifugation or pretreatment. Porcine blood was collected into aluminum foil–wrapped syringes via a 12-inch butterfly needle tube assembly. Two blood samples were collected from the same animal—one with the butterfly needle tubing wrapped in aluminum foil and one with the tubing exposed to ambient room light. The RSNO concentrations in these sequential blood samples were determined by a standard addition procedure. Results: Eight sets of measurements were made in 6 animals. Samples exposed to light yielded RSNO concentrations only 23.6% (7.2%) [mean (SD)] of the RSNO concentrations determined in samples that were shielded from light and obtained from the same animals. Conclusions: These results suggest significant photoinstablity of RSNOs in whole blood and indicate the critical importance of proper light protection during sampling and processing of blood samples for the accurate determinations of endogenous RSNO concentrations.

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