scholarly journals Development of a Modular Assay for Detailed Immunophenotyping of Peripheral Human Whole Blood Samples by Multicolor Flow Cytometry

2016 ◽  
Vol 17 (8) ◽  
pp. 1316 ◽  
Author(s):  
Paul Rühle ◽  
Rainer Fietkau ◽  
Udo Gaipl ◽  
Benjamin Frey
2021 ◽  
Vol 2 (1) ◽  
pp. 100311
Author(s):  
Daniella C. Terenzi ◽  
Ehab Bakbak ◽  
Justin Z. Trac ◽  
Mohammad Al-Omran ◽  
Adrian Quan ◽  
...  

1994 ◽  
Vol 42 (3) ◽  
pp. 231-241 ◽  
Author(s):  
C. Shenberg ◽  
S. Spiegel ◽  
S. Chaitchik ◽  
P. Jordan ◽  
M. Kitzis ◽  
...  

2014 ◽  
Vol 436 ◽  
pp. 72-77 ◽  
Author(s):  
Li-Ting Liao ◽  
Chi-Chih Liao ◽  
Chiu-Ching Liu ◽  
Ting-Ya Yang ◽  
Giueng-Chueng Wang

2001 ◽  
Vol 86 (09) ◽  
pp. 784-790 ◽  
Author(s):  
Catherine Vidal ◽  
Christian Spaulding ◽  
Françoise Picard ◽  
Frédéric Schaison ◽  
Josiane Melle ◽  
...  

SummaryPlatelet activation is known to participate to the pathogenesis of acute coronary syndromes. Aminophospholipid exposure and micro-particles shedding are hallmarks of full platelet activation and may account for the dissemination of prothrombotic seats. Using flow cytometry analysis of annexin V binding to externalized aminophospholipids, we followed platelet procoagulant activity (PPA) and platelet microparticles (PMP) shedding in venous and coronary whole blood samples from 30 patients with unstable angina before and after percutaneous coronary angioplasty (PTCA) and stent implantation. Baseline values of PPA and PMP were significantly more elevated in patients than in control subjects (p <0.005). PMP percentage was significantly higher in coronary than in venous blood, and in coronary blood of patients with proximal instead of mid/distal lesions of coronary arteries. No enhancement of platelet reactivity to TRAP and collagen was induced by procedure. Whereas activated GpIIb-IIIa and P-selectin expression decreased 24 h and 48 h after procedure, PPA and PMP remained as elevated as before. Thus, flow cytometry is a reliable method for detection of fully activated platelets in whole blood samples. Annexin V binding analysis demonstrates the persistance of in vivo platelet activation, despite the use of antiaggregating agents.


2011 ◽  
Vol 412 (23-24) ◽  
pp. 2150-2156 ◽  
Author(s):  
Ming-Song Hsieh ◽  
Tai-Guang Wu ◽  
Chein-Shyong Su ◽  
Wen-Jing Cheng ◽  
Namik Ozbek ◽  
...  

2015 ◽  
Vol 51 (64) ◽  
pp. 12748-12751 ◽  
Author(s):  
Yuhui Wang ◽  
Kai Jiang ◽  
Jiali Zhu ◽  
Ling Zhang ◽  
Hengwei Lin

A novel FRET model employing fluorescent carbon dots and MnO2nanosheets as donor–acceptor pairs is built for GSH sensing.


2011 ◽  
Vol 3 (10) ◽  
pp. 652-660 ◽  
Author(s):  
Martin Schönfelder ◽  
Hande Hofmann ◽  
Patricia Anielski ◽  
Detlef Thieme ◽  
Renate Oberhoffer ◽  
...  

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