CTGF/Hcs24, hypertrophic chondrocyte-specific gene product, interacts with perlecan in regulating the proliferation and differentiation of chondrocytes

2003 ◽  
Vol 196 (2) ◽  
pp. 265-275 ◽  
Author(s):  
Takashi Nishida ◽  
Satoshi Kubota ◽  
Tomohiro Fukunaga ◽  
Seiji Kondo ◽  
Gen Yosimichi ◽  
...  
Keyword(s):  
Gene Product ◽  
Specific Gene ◽  
Hypertrophic Chondrocyte ◽  
Proliferation And Differentiation

scholarly journals Effects of CTGF/Hcs24, a Product of a Hypertrophic Chondrocyte-Specific Gene, on the Proliferation and Differentiation of Chondrocytes in Culture1

Endocrinology ◽  
2000 ◽  
Vol 141 (1) ◽  
pp. 264-273 ◽  
Author(s):  
Tohru Nakanishi ◽  
Takashi Nishida ◽  
Tsuyoshi Shimo ◽  
Kappei Kobayashi ◽  
Toshikazu Kubo ◽  
...  
Keyword(s):  
Specific Gene ◽  
Hypertrophic Chondrocyte ◽  
Proliferation And Differentiation

Involvement of CTGF, a Hypertrophic Chondrocyte-Specific Gene Product, in Tumor Angiogenesis

Oncology ◽  
2001 ◽  
Vol 61 (4) ◽  
pp. 315-322 ◽  
Author(s):  
Tsuyoshi Shimo ◽  
Tohru Nakanishi ◽  
Takashi Nishida ◽  
Masahiro Asano ◽  
Akira Sasaki ◽  
...  
Keyword(s):  
Gene Product ◽  
Tumor Angiogenesis ◽  
Specific Gene ◽  
Hypertrophic Chondrocyte

scholarly journals Yeast repressor alpha 2 binds to its operator cooperatively with yeast protein Mcm1.

1989 ◽  
Vol 9 (11) ◽  
pp. 5228-5230 ◽  
Author(s):  
C A Keleher ◽  
S Passmore ◽  
A D Johnson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Product ◽  
Strong Evidence ◽  
Regulatory Proteins ◽  
Yeast Cells ◽  
Specific Gene ◽  
Yeast Gene ◽  
Yeast Protein ◽  
Transcriptional Regulatory

To bring about repression of a family fo genes in Saccharomyces cerevisiae called the a-specific genes, two transcriptional regulatory proteins, alpha 2 and GRM (general regulator of matin type), bind cooperatively to an operator found upstream of each a-specific gene. To date, GRM has been defined only biochemically. In this communication we show that the product of a single yeast gene (MCM1) is sufficient to bind cooperatively with alpha 2 to the operator. We also show that antiserum raised against the MCM1 gene product recognizes GRM from yeast cells. These results, in combination with previous observations, provide strong evidence that MCM1 encodes the GRM activity.

scholarly journals Effects of 5-Aza-2’ Deoxycytidine on Proliferation and Differentiation of Embryonic Chick Caudal Region Chondrocytes in Culture

2016 ◽  
Author(s):  
Samina Hyder Haq
Keyword(s):  
Cellular Differentiation ◽  
Rna Synthesis ◽  
Optimum Concentration ◽  
Embryonic Chick ◽  
Caudal Region ◽  
Cell Basis ◽  
Hypertrophic Chondrocyte ◽  
Epigenetic Factor ◽  
Proliferation And Differentiation ◽  
Differentiation Status

DNA methylation is one of the epigenetic mechanisms which have been implicated in cellular differentiation, ageing and disease development. The effect of hypomethylating drug 5-aza-2’ deoxycytidine (5-aza dC) on the biosynthetic profile of caudal region chondrocytes from chick sternum was studied in detail. The chondrocytes in culture were treated with varying doses of 5-aza dC for 48h and maintained subsequently without the treatment and harvested at selected time points for analysis of growth and differentiation status. 15µg/ml of 5-aza dC showed optimum Concentration at which there was a significant increase in DNA synthesis and RNA synthesis as per cell basis. There was also a significant increase in total protein synthesis and collagen synthesis as per cell basis at this concentration. This optimal concentration also showed to up regulate the gene expression of Type X collagen and alkaline phosphatase, which are the marker of hypertrophic chondrocyte expression. These results further support the notion that methylation is the major epigenetic factor controlling the differentiation and maturation of chondrocytes

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