High glucose increase cell cycle regulatory proteins level of mouse embryonic stem cells via PI3-K/Akt and MAPKs signal pathways

2006 ◽  
Vol 209 (1) ◽  
pp. 94-102 ◽  
Author(s):  
Yun Hee Kim ◽  
Jung Sun Heo ◽  
Ho Jae Han
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Glucose ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Regulatory Proteins ◽  
Signal Pathways ◽  
Cell Cycle Regulatory Proteins

scholarly journals Rapid Actions of Plasma Membrane Estrogen Receptors Regulate Motility of Mouse Embryonic Stem Cells through a Profilin-1/Cofilin-1-Directed Kinase Signaling Pathway

2012 ◽  
Vol 26 (8) ◽  
pp. 1291-1303 ◽  
Author(s):  
Seung Pil Yun ◽  
Jung Min Ryu ◽  
Mi Ok Kim ◽  
Jae Hong Park ◽  
Ho Jae Han
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Motility ◽  
Estrogen Receptors ◽  
Combined Treatment ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Signal Pathways ◽  
Ici 182,780 ◽  
Actin Expression

Long-term estrogen actions are vital for driving cell growth, but more recent evidence suggests that estrogen mediates more rapid cellular effects. However, the function of estradiol-17β (E2)-BSA in mouse embryonic stem cells has not been reported. Therefore, we examined the role of E2-BSA in mouse embryonic stem cell motility and its related signal pathways. E2-BSA (10−8m) significantly increased motility after 24 h incubation and increased filamentous (F)-actin expression; these effects were inhibited by the estrogen receptor antagonist ICI 182,780, indicating that E2-BSA bound membrane estrogen receptors and initiated a signal. E2-BSA increased c-Src and focal adhesion kinase (FAK) phosphorylation, which was attenuated by ICI 182,780. The E2-BSA-induced increase in epidermal growth factor receptor (EGFR) phosphorylation was inhibited by Src inhibitor PP2. As a downstream signal molecule, E2-BSA activated cdc42 and increased formation of a complex with the neural Wiskott-Aldrich syndrome protein (N-WASP)/cdc42/transducer of cdc42-dependent actin assembly-1 (TOCA-1), which was inhibited by FAK small interfering RNA (siRNA) and EGFR inhibitor AG 1478. In addition, E2-BSA increased profilin-1 expression and cofilin-1 phosphorylation, which was blocked by cdc42 siRNA. Subsequently, E2-BSA induced an increase in F-actin expression, and cell motility was inhibited by each signal pathway-related siRNA molecule or inhibitors but not by cofilin-1 siRNA. A combined treatment of cofilin-1 siRNA and E2-BSA increased F-actin expression and cell motility more than that of E2-BSA alone. These data demonstrate that E2-BSA stimulated motility by interacting with profilin-1/cofilin-1 and F-actin through FAK- and c-Src/EGFR transactivation-dependent N-WASP/cdc42/TOCA-1 complex.

Alteration of gene expression profile in mouse embryonic stem cells and neural differentiation deficits by ethephon

2020 ◽  
Vol 39 (11) ◽  
pp. 1518-1527
Author(s):  
S Mohammadi Nejad ◽  
M Hodjat ◽  
SA Mousavi ◽  
M Baeeri ◽  
MA Rezvanfar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Differentiation Potential

Ethephon, a member of the organophosphorus compounds, is one of the most widely used plant growth regulators for artificial ripening. Although million pounds of this chemical is being used annually, the knowledge regarding its molecular toxicity is yet not sufficient. The purpose of this study was to evaluate the potential developmental toxicity of ethephon using embryonic stem cell model. The mouse embryonic stem cells (mESCs) were exposed to various concentrations of ethephon and the viability, cell cycle alteration and changes in the gene expression profile were evaluated using high-throughput RNA sequencing. Further, the effect of ethephon on neural differentiation potential was examined. The results showed that ethephon at noncytotoxic doses induced cell cycle arrest in mESCs. Gene ontology enrichment analysis showed that terms related to cell fate and organismal development, including neuron fate commitment, embryo development and cardiac cell differentiation, were markedly enriched in ethephon-treated cells. Neural induction of mESCs in the presence of ethephon was inhibited and the expression of neural genes was decreased in differentiated cells. Results obtained from this work clearly demonstrate that ethephon affects the gene expression profile of undifferentiated mESCs and prevents neural differentiation. Therefore, more caution against the frequent application of ethephon is advised.

The miR-290-295 cluster promotes pluripotency maintenance by regulating cell cycle phase distribution in mouse embryonic stem cells

2011 ◽  
Vol 81 (1) ◽  
pp. 11-24 ◽  
Author(s):  
Zsuzsanna Lichner ◽  
Emőke Páll ◽  
Andrea Kerekes ◽  
Éva Pállinger ◽  
Pouneh Maraghechi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Phase Distribution ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Cell Cycle Phase Distribution ◽  
Pluripotency Maintenance

scholarly journals Rad51 Regulates Cell Cycle Progression by Preserving G2/M Transition in Mouse Embryonic Stem Cells

2014 ◽  
Vol 23 (22) ◽  
pp. 2700-2711 ◽  
Author(s):  
Sang-Wook Yoon ◽  
Dae-Kwan Kim ◽  
Keun Pil Kim ◽  
Kyung-Soon Park
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Cycle Progression ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cycle Progression

scholarly journals Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ariel Waisman ◽  
Federico Sevlever ◽  
Martín Elías Costa ◽  
María Soledad Cosentino ◽  
Santiago G. Miriuka ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Ground State ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cell Cycle Dynamics

Analysis of the Cell Cycle in Mouse Embryonic Stem Cells

2003 ◽  
pp. 27-33 ◽  
Author(s):  
Pierre Savatier ◽  
Hélène Lapillonne ◽  
Ludmila Jirmanova ◽  
Luigi Vitelli ◽  
Jacques Samarut
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells
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