In‐situ product recovery as a strategy for bioprocess integration and depletion of inhibitory products

2021 ◽  
Author(s):  
Ulises A. Salas‐Villalobos ◽  
Rígel V. Gómez‐Acata ◽  
Josefina Castillo‐Reyna ◽  
Oscar Aguilar
Keyword(s):  
Product Recovery ◽  
In Situ Product Recovery

Overcoming the drawbacks of microsieves with micromeshes for in situ product recovery

2013 ◽  
Vol 436 ◽  
pp. 16-27 ◽  
Author(s):  
F. Carstensen ◽  
T. Kasperidus ◽  
M. Wessling
Keyword(s):  
Product Recovery ◽  
In Situ Product Recovery

scholarly journals A comparison of the energy use of in situ product recovery techniques for the Acetone Butanol Ethanol fermentation

2016 ◽  
Vol 220 ◽  
pp. 590-600 ◽  
Author(s):  
Victoria Outram ◽  
Carl-Axel Lalander ◽  
Jonathan G.M. Lee ◽  
E. Timothy Davis ◽  
Adam P. Harvey
Keyword(s):  
Ethanol Fermentation ◽  
Energy Use ◽  
Product Recovery ◽  
In Situ Product Recovery ◽  
Recovery Techniques ◽  
Acetone Butanol Ethanol

Selection of eco-efficient downstream distillation sequences for acetone-butanol-ethanol (ABE) purification from in situ product recovery system

2021 ◽  
Author(s):  
Gen Liu ◽  
Zhihao Si ◽  
Bo Chen ◽  
Changjing Chen ◽  
Shikun Cheng ◽  
...  
Keyword(s):  
Product Recovery ◽  
In Situ Product Recovery ◽  
Recovery System ◽  
Acetone Butanol Ethanol ◽  
Selection Of

scholarly journals Improved Production and In Situ Recovery of Sesquiterpene (+)-Zizaene from Metabolically-Engineered E. coli

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3356 ◽  
Author(s):  
Francisco Aguilar ◽  
Thomas Scheper ◽  
Sascha Beutel
Keyword(s):  
Liquid Phase ◽  
Product Recovery ◽  
Expanded Bed Adsorption ◽  
Biotechnological Production ◽  
Promising Alternative ◽  
Phase Partitioning ◽  
In Situ Product Recovery ◽  
Solid Liquid ◽  
In Situ Recovery

The sesquiterpene (+)-zizaene is the direct precursor of khusimol, the main fragrant compound of the vetiver essential oil from Chrysopogon zizanioides and used in nearly 20% of men’s fine perfumery. The biotechnological production of such fragrant sesquiterpenes is a promising alternative towards sustainability; nevertheless, product recovery from fermentation is one of the main constraints. In an effort to improve the (+)-zizaene recovery from a metabolically-engineered Escherichia coli, we developed an integrated bioprocess by coupling fermentation and (+)-zizaene recovery using adsorber extractants. Initially, (+)-zizaene volatilization was confirmed from cultivations with no extractants but application of liquid–liquid phase partitioning cultivation (LLPPC) improved (+)-zizaene recovery nearly 4-fold. Furthermore, solid–liquid phase partitioning cultivation (SLPPC) was evaluated by screening polymeric adsorbers, where Diaion HP20 reached the highest recovery. Bioprocess was scaled up to 2 L bioreactors and in situ recovery configurations integrated to fermentation were evaluated. External recovery configuration was performed with an expanded bed adsorption column and improved (+)-zizaene titers 2.5-fold higher than LLPPC. Moreover, internal recovery configuration (IRC) further enhanced the (+)-zizaene titers 2.2-fold, whereas adsorption velocity was determined as critical parameter for recovery efficiency. Consequently, IRC improved the (+)-zizaene titer 8.4-fold and productivity 3-fold from our last report, achieving a (+)-zizaene titer of 211.13 mg L−1 and productivity of 3.2 mg L−1 h−1. This study provides further knowledge for integration of terpene bioprocesses by in situ product recovery, which could be applied for many terpene studies towards the industrialization of fragrant molecules.

Improved enzymatic synthesis of N-carbamoyl-D-phenylalanine with in situ product recovery

2011 ◽  
Vol 16 (3) ◽  
pp. 611-616 ◽  
Author(s):  
Z. Zhou ◽  
Z. Yao ◽  
H. Q. Wang ◽  
H. Xu ◽  
P. Wei ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Product Recovery ◽  
In Situ Product Recovery

Development of reactive extraction systems for itaconic acid: a step towards in situ product recovery for itaconic acid fermentation

RSC Advances ◽  
2014 ◽  
Vol 4 (85) ◽  
pp. 45029-45039 ◽  
Author(s):  
Guneet Kaur ◽  
Kathy Elst
Keyword(s):  
Process Optimization ◽  
Itaconic Acid ◽  
Acid Production ◽  
Product Recovery ◽  
Reactive Extraction ◽  
Acid Fermentation ◽  
In Situ Product Recovery ◽  
Product Separation ◽  
Important Means

Process optimization by integration of bioconversion with product separation and recovery i.e. in situ product recovery (ISPR) is an important means to develop a sustainable and petrochemical-competitive biotechnological method for itaconic acid production.

In situ product recovery of single-chain antibodies in a membrane bioreactor

2014 ◽  
Vol 111 (8) ◽  
pp. 1566-1576 ◽  
Author(s):  
Kristina Meier ◽  
Frederike Carstensen ◽  
Christoph Scheeren ◽  
Lars Regestein ◽  
Matthias Wessling ◽  
...  
Keyword(s):  
Membrane Bioreactor ◽  
Product Recovery ◽  
Single Chain ◽  
In Situ Product Recovery ◽  
Single Chain Antibodies

Reverse-flow diafiltration for continuous in situ product recovery

2012 ◽  
Vol 421-422 ◽  
pp. 39-50 ◽  
Author(s):  
Frederike Carstensen ◽  
Christian Marx ◽  
João André ◽  
Thomas Melin ◽  
Matthias Wessling
Keyword(s):  
Reverse Flow ◽  
Product Recovery ◽  
In Situ Product Recovery

scholarly journals Enhanced Production and in situ Product Recovery of Fusicocca-2,10(14)-Diene from Yeast

Fermentation ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. 65 ◽  
Author(s):  
Lisa Halka ◽  
Rolf Wichmann
Keyword(s):  
Chemical Synthesis ◽  
Oxygen Supply ◽  
Carbon Sources ◽  
Product Recovery ◽  
Yeast Fermentation ◽  
In Situ Extraction ◽  
In Situ Product Recovery ◽  
Enhanced Production ◽  
Oxygen Conditions

Fusicocca-2,10(14)-diene (FCdiene) is a tricyclic diterpene which has many pharmaceutical applications, for example, it is a precursor for different anticancer drugs, including fusicoccin A. Chemical synthesis of this diterpene is not economical as it requires 14 steps with several stereospecific reactions. FCdiene is naturally produced at low titers in phytopathogenic filamentous fungi. However, production of FCdiene can be achieved via expression of fusicoccadiene synthase in yeast. The objective of this study is to increase FCdiene production by optimizing the yeast fermentation process. Our preliminary fermentations showed influences of carbon sources, buffer agents, and oxygen supply on FCdiene production. Buffer agents as well as oxygen supply were investigated in detail at 0.2 and 1.8 L cultivation volumes. Using glucose as the carbon source, FCdiene concentrations were increased to 240 mgFCdiene/L by optimizing pH and oxygen conditions. In situ extraction and adsorption techniques were examined at the 0.2 L scale to determine if these techniques could improve FCdiene yields. Different adsorbents and solvents were tested with in situ product recovery and 4-fold increases in FCdiene productivity could be shown. The results generated in this work provide a proof-of-concept for the fermentative production of FCdiene from S. cerevisiae as a practical alternative to chemical synthesis.

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