Analysis of inductive agents in chick embryo extract usingTriturus gastrula ectoderm as reactor

1960 ◽  
Vol 144 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Izumi Kawakami ◽  
Sumiko Iyeiri ◽  
Naoi Sasaki
Keyword(s):  
Chick Embryo ◽  
Embryo Extract ◽  
Chick Embryo Extract

scholarly journals EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE

1974 ◽  
Vol 61 (2) ◽  
pp. 369-382 ◽  
Author(s):  
D. A. Newsome ◽  
R. T. Fletcher ◽  
W. G. Robison ◽  
K. R. Kenyon ◽  
G. J. Chader
Keyword(s):  
Molecular Weight ◽  
Chick Embryo ◽  
Adenosine Monophosphate ◽  
Cell Number ◽  
Retinal Pigmented Epithelium ◽  
Culture Dish ◽  
Adhesive Properties ◽  
Embryo Extract ◽  
Chick Embryo Extract ◽  
Pigmented Epithelium

The effects of dibutyryl cyclic 3',5'-adenosine monophosphate (BcAMP) and Sephadex G-25 fractions of chick embryo extract on the growth rate, morphology, and pigmentation of normal chick retinal pigmented epithelium (PE) were investigated. Seven cloned PE cell lines were each grown in modified Ham's F-12 medium alone (F-12), or in F-12 supplemented with either high molecular weight (H) or low molecular weight (L) fractions of chick embryo extract. Cells grown in F-12 alone or in L medium formed compact epithelial sheets, whereas cells grown in H had a fibrocytic appearance and formed poorly organized monolayers. In H plus BcAMP, cell morphology was more epithelioid than in H alone, and generally the monolayers appeared more differentiated. Under each of these three culture conditions, 2 x 10-4 M BCAMP retarded the increase in cell number and decreased the final number of cells per culture dish, but had little effect on plating efficiency. BcAMP also increased the rate of cell adhesion to a plastic substratum. Pigmentation was marked in cultures grown in F-12 or in L alone, but the addition of BcAMP dramatically reduced visible pigmentation. This effect was reversed when BcAMP was removed from the culture medium. Thus BcAMP modifies cell and colonial morphology, rate of cell accumulation, adhesive properties, and pigmentation of normal PE cells.

A Culture Technique Using Marine Fish Kidney to Obtain Chromosomes

1979 ◽  
Vol 36 (4) ◽  
pp. 458-461 ◽  
Author(s):  
Eun Ho Park ◽  
Sang Dai Park
Keyword(s):  
Chick Embryo ◽  
Marine Fish ◽  
Culture Technique ◽  
Kidney Cells ◽  
High Concentration ◽  
Embryo Extract ◽  
Key Words Kidney ◽  
Chick Embryo Extract ◽  
Conger Eel

A relatively simple and reliable in vitro method for marine fish chromosome study was developed. The addition of 10% chick embryo extract to serum-supplemented Eagle's minimum essential medium with high concentration of NaCl resulted in marked growth of kidney cells in the marine conger eel (Astroconger myriaster) after activation by phytohemagglutinin (PHA). Culture medium without chick embryo extract or PHA and/or with normal concentration of NaCl did not induce substantial growth. In contrast to reports by others, humidified culture was not required for excellent cell growth of these teleost kidney cells. Numerous metaphases unmarred by overlapping chromosomes were recovered and excellent karyograms were available for detailed karyotype analysis. Key words: kidney, culture, marine fish, chromosome

scholarly journals THE EFFECT OF LYMPHOCYTOSIS INDUCED WITH EMBRYONIC EXTRACT ON THE COURSE OF EXPERIMENTAL TUBERCULOSIS IN RABBITS

1932 ◽  
Vol 56 (2) ◽  
pp. 173-183 ◽  
Author(s):  
Kenneth C. Smithburn
Keyword(s):  
Chick Embryo ◽  
Lymph Nodes ◽  
Blood Cells ◽  
Lymphoid Hyperplasia ◽  
Germinal Centers ◽  
Experimental Tuberculosis ◽  
Embryo Extract ◽  
Chick Embryo Extract ◽  
Tuberculosis In Children ◽  
Immature Cells

1. Chick embryo extract given intravenously did not favorably influence the course of experimental tuberculosis in rabbits, although it did cause lymphocytosis and lymphoid hyperplasia. 2. The tuberculous animals treated with chick embryo extract showed lower values for hemoglobin and red blood cells than tuberculous controls inoculated from the same suspension. 3. Under the conditions of these experiments an increase in the number of young, immature cells in the lymph nodes was accompanied by more extensive tuberculous lesions in the lymph nodes and especially in the germinal centers of the nodes. A possible analogy to lymphatic tuberculosis in children is suggested. 4. The observations of previous investigators on the progression and later regression of lesions in experimental tuberculosis have been confirmed. The distribution, character, and extent of lesions have been studied and are presented in tabular form. 5. The presence and significance of tuberculous thrombi in the lungs are discussed.

scholarly journals In Vitro Survival and Multiplication of Chicken Myeloblasts Promoted for Several Weeks by Chick Embryo Extract

1995 ◽  
Vol 74 (6) ◽  
pp. 942-950 ◽  
Author(s):  
CAROLLE NICOLAS-BOLNET ◽  
F. DIETERLEN-LIEVRE
Keyword(s):  
Chick Embryo ◽  
Embryo Extract ◽  
Chick Embryo Extract ◽  
In Vitro Survival

Synergism of nucleoprotein and dialysate growth factors in chick embryo extract

1954 ◽  
Vol 6 (2) ◽  
pp. 327-336 ◽  
Author(s):  
M. Harris ◽  
R.J. Kutsky
Keyword(s):  
Growth Factors ◽  
Chick Embryo ◽  
Embryo Extract ◽  
Chick Embryo Extract

scholarly journals THE EFFECT OF CHICK EMBRYO EXTRACT ON THE GROWTH AND MORPHOLOGY OF TUBERCLE BACILLI

1948 ◽  
Vol 88 (3) ◽  
pp. 355-360 ◽  
Author(s):  
Hubert Bloch
Keyword(s):  
Oleic Acid ◽  
Chick Embryo ◽  
Aqueous Extracts ◽  
Chick Embryos ◽  
Embryo Extract ◽  
Virulent Strains ◽  
Chick Embryo Extract

Aqueous extracts of 11-day-old chick embryos enhance the growth of tubercle bacilli in oleic acid-albumin media. These extracts also increase the tendency of virulent strains to exhibit the serpentine pattern of growth and confer this property on avirulent variants which normally grow unoriented in clumps. Growth in the presence of chick embryo extract slightly increases the virulence of the virulent strains but does not confer virulence on the avirulent variants.

scholarly journals Stimulation of collagen galactosyltransferase and glucosyltransferase activities by lysophosphatidylcholine

1976 ◽  
Vol 160 (1) ◽  
pp. 29-35 ◽  
Author(s):  
H Anttinen
Keyword(s):  
Chick Embryo ◽  
High Speed ◽  
Membrane Structures ◽  
Embryo Extract ◽  
Chick Embryo Extract ◽  
Triton X ◽  
Regulatory Significance ◽  
Stimulation Of

Lysophosphatidylcholine stimulated the activities of collagen galactosyl- and glucosyl-transferases in chick-embryo extract and its particulate fractions in vitro, whereas essentially no stimulation was noted in the high-speed supernatant, where the enzymes are soluble and membrane-free. The stimulatory effect of lysophosphatidylcholine was masked by 0.1% Triton X-100. In kinetic experiments lysophosphatidylcholine raised the maximum velocities with respect to the substrates and co-substrates, whereas no changes were observed in the apparant Km values. Phospholipase A preincubation of the chick-embryo extract resulted in stimulation of both transferase activities, probably gy generating lysophosphatides from endogenous phospholipids. No stimulation by lysophosphatidylcholine was found when tested with 500-fold-purified glycosyltransferase. The results suggest that collagen glycosyltransferases must be associated with the membrane structures of the cell in order to be stimulated by lysophosphatidylcholine. Lysophosphatidylcholine could have some regulatory significance in vivo, since its concentration in the cell is comparable with that which produced marked stimulation in vitro.

Control of myogenesis in vitro by chick embryo extract

1976 ◽  
Vol 50 (2) ◽  
pp. 264-284 ◽  
Author(s):  
C.R. Slater
Keyword(s):  
Chick Embryo ◽  
Embryo Extract ◽  
Chick Embryo Extract

scholarly journals Chick Embryo Extract, Muscle Trophic Factor and Chick and Horse Sera as Environments for Chick Myogenic Cell Growth

1981 ◽  
Vol 23 (3) ◽  
pp. 249-254 ◽  
Author(s):  
YASUKO HAGIWARA ◽  
ICHIRO KIMURA ◽  
EIJIRO OZAWA
Keyword(s):  
Cell Growth ◽  
Chick Embryo ◽  
Myogenic Cell ◽  
Trophic Factor ◽  
Embryo Extract ◽  
Chick Embryo Extract
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