Quantitation of fatty acids and hydroxy fatty acids by gas chromatography/mass spectrometry. Predictively useful correlations of relative response factors with empirical formula

1995 ◽  
Vol 30 (7) ◽  
pp. 1018-1022 ◽  
Author(s):  
Chandra S. Chaurasia ◽  
Todd D. Williams ◽  
Charles M. Judson ◽  
Robert P. Hanzlik
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Empirical Formula ◽  
Hydroxy Fatty Acids ◽  
Gas Chromatography Mass Spectrometry ◽  
Response Factors ◽  
Relative Response ◽  
Chromatography Mass Spectrometry

Determining double-bond positions in monoenoic 2-hydroxy fatty acids of glucosylceramides by gas chromatography-mass spectrometry

Lipids ◽  
2000 ◽  
Vol 35 (2) ◽  
pp. 233-236 ◽  
Author(s):  
Hiroyuki Imai ◽  
Kohei Yamamoto ◽  
Akira Shibahara ◽  
Shuichi Miyatani ◽  
Takao Nakayama
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Double Bond ◽  
Hydroxy Fatty Acids ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

scholarly journals Improved Stable Isotope Dilution-Gas Chromatography-Mass Spectrometry Method for Serum or Plasma Free 3-Hydroxy-Fatty Acids and Its Utility for the Study of Disorders of Mitochondrial Fatty Acid β-Oxidation

2000 ◽  
Vol 46 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Patricia M Jones ◽  
Rebecca Quinn ◽  
Paul V Fennessey ◽  
Susan Tjoa ◽  
Stephen I Goodman ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Stable Isotope ◽  
Isotope Dilution ◽  
Hydroxy Fatty Acids ◽  
Gas Chromatography Mass Spectrometry ◽  
Stable Isotope Dilution ◽  
Chromatography Mass Spectrometry

Abstract Background: Disorders of fatty acid oxidation (FAO) are difficult to diagnose, primarily because in many of the FAO disorders measurable biochemical intermediates accumulate in body fluids only during acute illness. Increased concentrations of 3-hydroxy-fatty acids (3-OH-FAs) in the blood are indicative of FAO disorders of the long- and short-chain 3-hydroxy-acyl-CoA dehydrogenases, LCHAD and SCHAD. We describe a serum/plasma assay for the measurement of 3-OH-FAs with carbon chain lengths from C6 to C16. Methods: We used stable isotope dilution gas chromatography-mass spectrometry (GC-MS) with electron impact ionization and selected ion monitoring. Natural and isotope-labeled compounds were synthesized for the assay. Results: The assay was linear from 0.2 to 50 μmol/L for all six 3-OH-FAs. CVs were 5–15% at concentrations near the upper limits seen in healthy subjects. In 43 subjects, the medians (and ranges) in μmol/L were as follows: 3-OH-C6, 0.8 (0.3–2.2); 3-OH-C8, 0.4 (0.2–1.0); 3-OH-C10, 0.3 (0.2–0.6); 3-OH-C12, 0.3 (0.2–0.6); 3-OH-C14, 0.2 (0.0–0.4); and 3-OH-C16, 0.2 (0.0–0.5). 3-OH-FAs were increased in infants receiving formula containing medium chain triglycerides. Two patients diagnosed with LCHAD deficiency showed marked increases in 3-OH-C14 and 3-OH-C16 concentrations. Two patients diagnosed with SCHAD deficiency showed increased shorter chain 3-OH-FAs but no increases in 3-OH-C14 to 3-OH-C16. Conclusion: Measuring blood concentrations of the 3-OH-FAs with this assay may be a valuable tool for helping to rapidly identify deficiencies in LCHAD and SCHAD and may also provide useful information about the status of the FAO pathway.

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