Characterization of complex, heterogeneous lipid A samples using HPLC-MS/MS technique II. Structural elucidation of non-phosphorylated lipid A by negative-ion mode tandem mass spectrometry

2016 ◽  
Vol 51 (8) ◽  
pp. 615-628 ◽  
Author(s):  
Viktor Sándor ◽  
Anikó Kilár ◽  
Ferenc Kilár ◽  
Béla Kocsis ◽  
Ágnes Dörnyei
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Lipid A ◽  
Structural Elucidation ◽  
Negative Ion ◽  
Tandem Mass ◽  
Negative Ion Mode

Identification of carbonylated peptides by tandem mass spectrometry using a precursor ion-like scan in negative ion mode

2011 ◽  
Vol 74 (11) ◽  
pp. 2351-2359 ◽  
Author(s):  
Ravi Ch. Bollineni ◽  
Maria Fedorova ◽  
Ralf Hoffmann
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Negative Ion ◽  
Tandem Mass ◽  
Negative Ion Mode

Characterization of stress degradation products of blonanserin by UPLC-QTOF-tandem mass spectrometry

RSC Advances ◽  
2015 ◽  
Vol 5 (85) ◽  
pp. 69273-69288 ◽  
Author(s):  
Pradipbhai D. Kalariya ◽  
Prinesh N. Patel ◽  
Mahesh Sharma ◽  
Prabha Garg ◽  
R. Srinivas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Tandem Mass Spectrometry ◽  
Degradation Products ◽  
Structural Elucidation ◽  
Forced Degradation ◽  
Tandem Mass ◽  
Degradation Study ◽  
Stress Degradation

Forced degradation study of blonanserin and structural elucidation of its degradation products was performed using high resolution tandem mass spectrometry.

Characterization of Oligosaccharides of the Lactosamine Series Derived from Keratan Sulfates by Tandem Mass Spectrometry

2000 ◽  
Vol 6 (2) ◽  
pp. 193-203 ◽  
Author(s):  
Masayuki Kubota ◽  
Keiichi Yoshida ◽  
Akira Tawada ◽  
Mamoru Ohashi
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Bond Cleavage ◽  
The Other ◽  
Negative Ion ◽  
Ring Cleavage ◽  
Tandem Mass ◽  
Fragment Ions ◽  
Glycosidic Bond Cleavage

Positive- and negative-ion fast-atom bombardment tandem mass spectrometry with collision-induced dissociation (FAB-CID-MS/MS) has been used in the characterization of di-and tetra-saccharides of the lactosamine series from keratan sulfates. FAB-CID-MS/MS of Galβ1-4GlcNAc (L1) exhibited strong fragment ions originating from ring cleavage at the reducing-terminal sugar moiety together with glycosidic bond-cleavage ions, whereas GlcNAcβ1-3Gal (K1) showed strong glycosidic bond-cleavage ions but no ring-cleavage ions. A series of ring-cleavage fragment ions was observed with members of the L-series which have free hydroxyl groups at the C1 and C3 positions. CID-MS/MS spectra of the [M + Na – SO3]+ ion ( m/z 406) from L2 and the [M + Na − 2SO3]+ ion ( m/z 406) from L4 were almost identical with the CID-MS/MS spectrum of the [M + Na]+ ion ( m/z 406) from L1, which indicated that the sugar skeletons of L2 and L4 are the same as that of L1. On the other hand, the CID-MS/MS spectrum of the [M + Na – SO3]+ ion ( m/z 508) from L4 did not resemble that of the [M + Na]+ ion ( m/z 508) from L2. The former showed peaks that were additional to the peaks in the latter. Since these extra peaks were accounted for on the basis of the structure of L3 [Galβ1(6S)-4GlcNAc, S = sulfate], the in-source loss of sulfate groups by ester exchange upon FAB ionization takes place in a dual manner; one reaction at the non-reducing terminal sugar to give L2 and the other at the reducing-terminal sugar to give L3. The CID-MS/MS spectra were characteristic for the tetrasaccharides L1-L1, L2-L2 and L4-L4 while in-source fragmentation confirms the component disaccharides of each tetrasaccharide. The structure of a tetrasaccharide trisulfate was confirmed as L2–L4 and not L4–L2 by CID-MS/MS. Negative-ion FAB-CID-MS/MS spectra of the sulfated di-and tetra-saccharides showed a pattern similar to that of the positive-ion spectra. Subtraction of the CID-MS/MS spectrum of the [M – H]− ion of L2 [Galβ1-4GlcNAc(6S)] from that of the [M – H – SO3]− ion of L4 [Gal(6S)β1-4GlcNAc(6S)] gave several specific ions whose origins were nicely explained on the basis of the structure of L3. The structure of a pentasaccharide consisting of N-acetylneuraminic acid and a tetrasaccharide trisulfate was confirmed, on the basis of FAB-CID-MS/MS, as NeuNAcα2-6L2-L4.

Simple extraction method using syringe filter for detection of ethephon in tomatoes by negative-ion mode liquid chromatography with tandem mass spectrometry

2015 ◽  
Vol 29 (10) ◽  
pp. 1480-1485 ◽  
Author(s):  
Soon-Kil Cho ◽  
Ji-Mi Cho ◽  
A. M. Abd El-Aty ◽  
Md. Musfiqur Rahman ◽  
Jeong-Heui Choi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Extraction Method ◽  
Negative Ion ◽  
Tandem Mass ◽  
Simple Extraction ◽  
Syringe Filter ◽  
Negative Ion Mode
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