Detection of EBV and HPV DNA sequences in oral “hairy” leukoplakia by in situ hybridization

1988 ◽  
Vol 26 (3) ◽  
pp. 271-277 ◽  
Author(s):  
L. R. Eversole ◽  
C. E. Stone ◽  
A. M. Beckman
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequences ◽  
Hpv Dna ◽  
Oral Hairy Leukoplakia ◽  
Hairy Leukoplakia

scholarly journals Diagnosis of Oral Hairy Leukoplakia: The Importance of EBV In Situ Hybridization

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Luana L. Martins ◽  
José Henrique F. Rosseto ◽  
Natália Silva Andrade ◽  
Juliana Bertoldi Franco ◽  
Paulo Henrique Braz-Silva ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Gold Standard ◽  
Epstein Barr Virus ◽  
Definitive Diagnosis ◽  
Barr Virus ◽  
Oral Hairy Leukoplakia ◽  
Hairy Leukoplakia ◽  
Epstein Barr ◽  
Epidemiological Characteristics

Oral hairy leukoplakia (OHL) is caused by the Epstein-Barr virus (EBV), which has been related to HIV infection. In situ hybridization (ISH) is the gold-standard diagnosis of OHL, but some authors believe in the possibility of performing the diagnosis based on clinical basis. The aim of this study is diagnose incipient lesions of OHL by EBV ISH of HIV-infected patients and the possible correlations with clinical characteristics of the patients. Ninety-four patients were examined and those presenting with clinical lesions compatible to OHL were submitted to biopsy prior to EBV ISH. Twenty-eight patients had lesions clinically compatible to the diagnosis of OHL, but only 20 lesions were confirmed by EBV ISH. The patients with OHL had a mean age of 41.9 years and were HIV-infected for 11.2 years, on average, including CD4 count of 504.7 cells/mm3 and log10 viral load = 1.1. Among the quantitative variables, there was a statistically significant correlation with age only (P=0.030). In conclusion, the presence of OHL in patients with HIV/AIDS results in changes in the epidemiological characteristics of the disease, and this fact allied with subtle clinical-morphological features makes clinical diagnosis very difficult. Therefore, EBV ISH is important for a definitive diagnosis of OHL.

Comparison of cytopathic changes in oral hairy leukoplakia with in situ hybridization for EBV DNA

Oral Diseases ◽  
2008 ◽  
Vol 4 (2) ◽  
pp. 95-99 ◽  
Author(s):  
JS Greenspan ◽  
YG Souza ◽  
JA Regezi ◽  
TE Daniels ◽  
D Greenspan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Oral Hairy Leukoplakia ◽  
Hairy Leukoplakia ◽  
Ebv Dna

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences

2011 ◽  
Vol 30 (9) ◽  
pp. 1779-1786 ◽  
Author(s):  
Kun Yang ◽  
Hecui Zhang ◽  
Richard Converse ◽  
Yong Wang ◽  
Xiaoying Rong ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Single Copy ◽  
Repetitive Dna Sequences
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