Knockdown of amyloid precursor protein normalizes cholinergic function in a cell line derived from the cerebral cortex of a trisomy 16 mouse: An animal model of down syndrome

2006 ◽  
Vol 84 (6) ◽  
pp. 1303-1310 ◽  
Author(s):  
Patricia Opazo ◽  
Katherine Saud ◽  
Michelle de Saint Pierre ◽  
Ana María Cárdenas ◽  
David D. Allen ◽  
...  
Keyword(s):  
Animal Model ◽  
Down Syndrome ◽  
Cerebral Cortex ◽  
Amyloid Precursor Protein ◽  
Cell Line ◽  
Precursor Protein ◽  
Trisomy 16 ◽  
Cholinergic Function ◽  
A Cell

Apoptosis is directly related to intracellular amyloid accumulation in a cell line derived from the cerebral cortex of a trisomy 16 mouse, an animal model of Down syndrome

2010 ◽  
Vol 470 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Christian Arriagada ◽  
Miguel Bustamante ◽  
Illani Atwater ◽  
Eduardo Rojas ◽  
Raúl Caviedes ◽  
...  
Keyword(s):  
Animal Model ◽  
Down Syndrome ◽  
Cerebral Cortex ◽  
Cell Line ◽  
Trisomy 16 ◽  
Amyloid Accumulation ◽  
A Cell

Altered Voltage Dependent Calcium Currents in a Neuronal Cell Line Derived From the Cerebral Cortex of a Trisomy 16 Fetal Mouse, an Animal Model of Down Syndrome

2011 ◽  
Vol 22 (1) ◽  
pp. 59-68 ◽  
Author(s):  
Mario A. Acuña ◽  
Ramón Pérez-Nuñez ◽  
Jorge Noriega ◽  
Ana María Cárdenas ◽  
Juan Bacigalupo ◽  
...  
Keyword(s):  
Animal Model ◽  
Down Syndrome ◽  
Cerebral Cortex ◽  
Cell Line ◽  
Neuronal Cell ◽  
Calcium Currents ◽  
Trisomy 16 ◽  
Fetal Mouse ◽  
Neuronal Cell Line ◽  
Voltage Dependent

Effect of the knockdown of amyloid precursor protein on intracellular calcium increases in a neuronal cell line derived from the cerebral cortex of a trisomy 16 mouse

2008 ◽  
Vol 209 (1) ◽  
pp. 234-242 ◽  
Author(s):  
Guillermo Rojas ◽  
Ana María Cárdenas ◽  
Paola Fernández-Olivares ◽  
Takeshi Shimahara ◽  
Juan Segura-Aguilar ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Amyloid Precursor Protein ◽  
Intracellular Calcium ◽  
Cell Line ◽  
Neuronal Cell ◽  
Precursor Protein ◽  
Trisomy 16 ◽  
Neuronal Cell Line

Regional alteration of cholinergic function in central neurons of trisomy 16 mouse fetuses, an animal model of human trisomy 21 (Down syndrome)

1994 ◽  
Vol 658 (1-2) ◽  
pp. 27-32 ◽  
Author(s):  
J.L. Fiedler ◽  
C.J. Epstein ◽  
S.I. Rapoport ◽  
R. Caviedes ◽  
P. Caviedes
Keyword(s):  
Animal Model ◽  
Down Syndrome ◽  
Trisomy 21 ◽  
Trisomy 16 ◽  
Cholinergic Function ◽  
Central Neurons ◽  
Mouse Fetuses

Neurohormetic responses of quercetin and rutin in a cell line over-expressing the amyloid precursor protein (APPswe cells)

Phytomedicine ◽  
2016 ◽  
Vol 23 (12) ◽  
pp. 1285-1294 ◽  
Author(s):  
Sagrario Martín-Aragón ◽  
Karim Lizeth Jiménez-Aliaga ◽  
Juana Benedí ◽  
Paloma Bermejo-Bescós
Keyword(s):  
Amyloid Precursor Protein ◽  
Cell Line ◽  
Precursor Protein ◽  
A Cell

Cell line construction and maintenance for Lyso-IP and Endo-IP analysis of amyloid precursor protein processing v1

2021 ◽  
Author(s):  
Hankum Park ◽  
Frances V Hundley ◽  
Harper JW
Keyword(s):  
Amyloid Precursor Protein ◽  
Cell Line ◽  
Quantitative Proteomics ◽  
Protein Processing ◽  
Precursor Protein ◽  
Amyloid Precursor Protein Processing ◽  
App Processing ◽  
293 Cells ◽  
Line Construction ◽  
App Gene

Lyso-IP is a method that allows for the isolation of lysosomes for proteomics and metabolomics (dx.doi.org/10.17504/protocols.io.bybjpskn; dx.doi.org/10.17504/protocols.io.bx9hpr36). We have developed an analogous approach for purification of early/sorting endosomes (Endo-IP). In addition, we have found that endolysosomal purification via Lyso-IP and Endo-IP can be coupled with a quantitative proteomics workflow to obtain snapshots of Amyloid Precursor Protein (APP) processing to its Aβ products (Park et al. in submission). Here, we describe methods for cell line construction and maintenance of 293 cells with TMEM192-3xHA and 3xFLAG-EEA1, which are used for lysosome and endosome purification, respectively, with the addition of patient mutations to APP promotes processing. Cells with endogenously tagged TMEM192 and stably expressing FLAG-EEA1 are referred to as 293EL cells, for Endo-IP and Lyso-IP. These cells were also prepared in a form that has a deletion of the APP gene (293EL;APP-/-) and the same cells reconstituted with a lentivirus stably expressing APPSw;T700N to allow functional analysis of APP processing.

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