Development of an isothermal recombinase aided amplification assay for the rapid and visualized detection of Klebsiella pneumoniae

2021 ◽  
Author(s):  
Laiwang Hou ◽  
Darong Li ◽  
Ni Zhang ◽  
Jiayi Zhao ◽  
Yong Zhao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Amplification Assay ◽  
Visualized Detection

scholarly journals A Recombinase Aided Amplification Assay for Rapid Detection of the Klebsiella pneumoniae Carbapenemase Gene and Its Characteristics in Klebsiella pneumoniae

2021 ◽  
Vol 11 ◽  
Author(s):  
Weiwei Zhang ◽  
Yanling Feng ◽  
Hanqing Zhao ◽  
Chao Yan ◽  
Junxia Feng ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Rapid Detection ◽  
Detection Method ◽  
Multidrug Resistant ◽  
Molecular Characteristics ◽  
Klebsiella Pneumoniae Carbapenemase ◽  
Carbapenem Resistant ◽  
Fecal Carriage ◽  
Carbapenemase Gene ◽  
Amplification Assay

Klebsiella pneumoniae carbapenemase genes (blaKPC) play an important role in carbapenem-resistant Enterobacteriaceae in China. A rapid detection method for blaKPC genes and investigations into the molecular characteristics of blaKPC positive Klebsiella pneumoniae were necessary. In this study, an easy and rapid recombinase aided amplification assay (RAA) for blaKPC was established. This protocol could be completed at 39°C in 15–20 min. The sensitivity of this assay was determined as 48 copies per reaction, and the specificity was 100%. The blaKPC RAA method could be used for clinical diagnosis and epidemiological investigation. Among 801 fecal samples from inpatients, 34 blaKPC positive isolates were identified from each sample, of which 23 isolates were K. pneumoniae. ST11 with blaKPC-2 was the most prevalent type. All these strains were multidrug resistant and carried various virulence genes. Fecal carriage of blaKPC positive carbapenem-resistant K.pneumoniae poses significant challenges for public health control.

scholarly journals Visual and Rapid Detection of Klebsiella pneumoniae by Magnetic Immunocapture-Loop-Mediated Isothermal Amplification Assay

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Liding Zhang ◽  
Yaoqiang Shi ◽  
Congje Chen ◽  
Qinqin Han ◽  
Mi Zhang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

scholarly journals Correlation between biofilm formation and carbapenem resistance among clinical isolates of Klebsiella pneumoniae

1970 ◽  
Vol 29 (6) ◽  
Author(s):  
Hossein Ali Rahdar ◽  
Ebadallah Shiri Malekabad ◽  
Ali-Reza Dadashi ◽  
Elahe Takei ◽  
Masuod Keikha ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Biofilm Formation ◽  
Severe Infection ◽  
Carbapenem Resistance ◽  
Clinical Isolates ◽  
P Value ◽  
Public Health Services ◽  
Carbapenem Resistant ◽  
Amplification Assay ◽  
Hospital Equipment

Background: Klebsiella pneumoniae is a Gram-negative enteric bacterium that causes nosocomial infections; this bacterium has survived from harsh condition using biofilm formation in hospital equipment and cause severe infection. In the other hand, the emergence and extension of carbapenem resistance burden among K. pneumonia producing biofilm is the current concern of public health services. There are controversial findings about this subject. The aim of this study was to evaluate the correlation between biofilm formation and resistance to carbapenem among clinical isolates of K. pneumoniae.Methods: A total of 160 K. pneumoniae isolates were collected from various infections of hospitalized patients. The Carba NP test and molecular methods were used for detection of carbapenem resistance isolates of K. pneumonia. Subsequently, the ability for biofilm production was performed from all isolates. Finally, Correlation of biofilm formation among carbapenem resistant isolates was calculated using χ2 and Fisher’s exact tests.Results: Among K. pneumoniae isolates 42.5% have carbapenemase activity by Carba NP test, while carbapenemase genes were detected in 35.6% of isolates in amplification assay. Moreover, there are 52.5% (n= 84) of all isolates were formed a strong biofilm, while 38.1% (n= 61) and 9.3% (n= 15) of isolates were middle and weak biofilm producer, respectively. Among carbapenem resistant cases (n= 68), there are 77.9% (n= 53) and 22% (n= 15) of isolates were reported as strong and middle biofilm producer, respectively. We see a significant correlation was seen between biofilm formation ability and carbapenem resistant isolates (p-value < 0.00001).Conclusion: The increase of carbapenem resistance burden in biofilm producing isolates of K. pneumoniae is considered as serious alert and the basic measures to combat this phenomenon is imperative.

Combination of Antibiotic Treatment with the Thrombin Inhibitor Recombinant Hirudin for the Therapy of Experimental Klebsiella pneumoniae Sepsis

1994 ◽  
Vol 71 (06) ◽  
pp. 768-772 ◽  
Author(s):  
Gerhard Dickneite ◽  
Jörg Czech
Keyword(s):  
Septic Shock ◽  
Klebsiella Pneumoniae ◽  
Organ Failure ◽  
Therapeutic Effect ◽  
Disseminated Intravascular Coagulation ◽  
Post Infection ◽  
Thrombin Inhibitor ◽  
Bolus Administration ◽  
Bacterial Burden ◽  
Recombinant Hirudin

SummaryRats which were infected with the gramnegative pathogen Klebsiella pneumoniae develop disseminated intravascular coagulation (DIC), multi-organ failure (MOF) and finally die in a septic shock. We investigated the therapeutic effect of antibiotic (tobramycin) treatment combined with the infusion of the highly specific thrombin inhibitor rec. hirudin. Although administration of 2 mg/kg tobramycin alone leads to a decrease of the bacterial burden, DIC could not be prevented. Infusion of rec. hirudin (0.25 mg/kg x h) for 4 h (start of treatment 1 h post infection), in addition to a bolus administration of tobramycin, led to an amelioration of DIC parameters as fibrinogen, thrombin-antithrombin complex (TAT) and platelets. Serum transaminase levels (GOT, GPT) as a marker of MOF were significantly improved by rec. hirudin, the T50 value increased from 17 h in the tobramycin group to 42 h in the tobramycin + rec. hirudin giuup, muilality rates were 90% or 60%, respectively. Combination of heparin (10011/kg x h) and tobramycin was not effective on survival.

Klebsiella pneumoniae – Erreger einer endogenen Endophthalmitis – Kasuistik

2004 ◽  
Vol 221 (S 5) ◽  
Author(s):  
A Scharipow ◽  
M Blum ◽  
K Ende ◽  
B Knau
Keyword(s):  
Klebsiella Pneumoniae

Catechol Siderophore Produced by Klebsiella Pneumoniae Isolated from Rhizosphere of Saccharum Officinarum L

2012 ◽  
Vol 2 (5) ◽  
pp. 423-425
Author(s):  
Shirishkumar V Supanekar ◽  
◽  
Ajay M Sorty ◽  
Avinash A Raut
Keyword(s):  
Klebsiella Pneumoniae ◽  
Saccharum Officinarum

Klebsiella pneumoniae – pięcioletnia analiza retrospektywna Centrum Oparzeniowego w Krakowie

2016 ◽  
Vol 4 (2) ◽  
pp. 47-53
Author(s):  
Anna Chrapusta ◽  
Roman Wach ◽  
Beata Śpiewak ◽  
Małgorzata Makowska ◽  
Paweł Palacz
Keyword(s):  
Klebsiella Pneumoniae
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