Suppression of the vacuolar invertase gene delays senescent sweetening in chipping potatoes

2017 ◽  
Vol 98 (1) ◽  
pp. 354-360 ◽  
Author(s):  
Amy E Wiberley-Bradford ◽  
Paul C Bethke
Keyword(s):  
Vacuolar Invertase ◽  
Invertase Gene ◽  
Chipping Potatoes

ALLELE DIVERSITY FOR ACID VACUOLAR INVERTASE GENE Pain-1 FRAGMENT IN POTATO (Solanum tuberosum L.) VARIETIES AND LINES

2018 ◽  
Vol 53 (1) ◽  
pp. 132-139
Author(s):  
M.A. Slugina ◽  
◽  
E.O. Shmelkova ◽  
A.A. Meleshin ◽  
E.Z. Kochieva ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Vacuolar Invertase ◽  
Invertase Gene

scholarly journals Suppression of the Vacuolar Invertase Gene Prevents Cold-Induced Sweetening in Potato

2010 ◽  
Vol 154 (2) ◽  
pp. 939-948 ◽  
Author(s):  
Pudota B. Bhaskar ◽  
Lei Wu ◽  
James S. Busse ◽  
Brett R. Whitty ◽  
Andy J. Hamernik ◽  
...  
Keyword(s):  
Vacuolar Invertase ◽  
Invertase Gene ◽  
Cold Induced Sweetening

Roles played by invertase and gene expression in the development of the horn-shaped gall on leaves of Rhus chinensis

2017 ◽  
Vol 44 (12) ◽  
pp. 1160 ◽  
Author(s):  
Zhen-Yuan Ruan ◽  
Xiao-Ming Chen ◽  
Pu Yang ◽  
Bing-Yi Wang
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Activity Level ◽  
Rapid Expansion ◽  
Sink Strength ◽  
Cell Wall Invertase ◽  
Vacuolar Invertase ◽  
Growing Period ◽  
Rhus Chinensis ◽  
Invertase Gene

The present study deals with the growth and development of the horn-shaped gall, which is induced by Schlechtendalia chinensis Bell. on leaves of Rhus chinensis Mill. The relationship between gall formers and their host plants was investigated by means of the activities of various invertases, the expressions of the cell wall invertase gene (INV2), and vacuolar invertase gene (INV3) during gall development. Our results show that the increase in the sink strength of the galls required cell wall invertase and vacuolar invertase, and that vacuolar invertase had a particular impact during the early development. In addition, vacuolar invertase activity was always significantly higher in galls than in leaves. However, ionically bound cell wall invertase showed a slightly significant increased activity level when compared with the leaves after galls had entered the fast growing period. This result indicates that vacuolar invertase is related to the rapid expansion of the galls, but ionically bound cell wall invertase is involved in the rapid growth of tissues. The enhanced activity of cell wall invertase and the expression of INV2 may be a plant response to a gall-induced stress. Cytoplasmic invertase that acts as a maintenance enzyme, or takes part in the production of secondary metabolites, was elevated when intracellular acid invertase activity decreased.

scholarly journals CsINV5, a tea vacuolar invertase gene enhances cold tolerance in transgenic Arabidopsis

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Wenjun Qian ◽  
Bin Xiao ◽  
Lu Wang ◽  
Xinyuan Hao ◽  
Chuan Yue ◽  
...  
Keyword(s):  
Cold Tolerance ◽  
Transgenic Arabidopsis ◽  
Vacuolar Invertase ◽  
Invertase Gene

Developing Cold-Chipping Potato Varieties by Silencing the Vacuolar Invertase Gene

Crop Science ◽  
2011 ◽  
Vol 51 (3) ◽  
pp. 981-990 ◽  
Author(s):  
Lei Wu ◽  
Pudota B. Bhaskar ◽  
James S. Busse ◽  
Ruofang Zhang ◽  
Paul C. Bethke ◽  
...  
Keyword(s):  
Vacuolar Invertase ◽  
Potato Varieties ◽  
Cold Chipping ◽  
Invertase Gene ◽  
Chipping Potato

Cloning and Analysis of Vacuolar Invertase Gene (MeVINV2) Promoter from Cassava (Manihot Esculenta Crantz)

2014 ◽  
Vol 618 ◽  
pp. 259-263
Author(s):  
Jiao Liu ◽  
Yan Ping Hu ◽  
Wen Rui Xia ◽  
Yuan Yao ◽  
Yang Zhou ◽  
...  
Keyword(s):  
Gene Promoter ◽  
Pcr Amplification ◽  
Promoter Sequence ◽  
Regulatory Elements ◽  
Starch Accumulation ◽  
Manihot Esculenta Crantz ◽  
Vacuolar Invertase ◽  
Biological Stress ◽  
Invertase Gene ◽  
Vacuolar Invertases

Vacuolar invertases play a vital role in the progress of cassava tuber roots starch accumulation. In order to study the regulating mechanism of cassava vacuolar invertases, the promoter of cassava vacuolar invertase 2 (MeVINV2) was isolated using the PCR amplification approach, starting with a part of coding sequences. Sequencing result showed that 47 bp MeVINV2 gene CDS sequence and 1242 bp potential promoter sequence was obtained. PlantCARE analysis revealed that the MeVINV2 gene promoter contains typical eukaryotic elements CAAT box and TATA box, and also several light-responsive elements and stress-responsive elements. These cis-acting regulatory elements might be associated to the vacuolar invertase gene function of cassava starch accumulation and biological stress defense.

scholarly journals Vacuolar Invertase Gene Silencing in Potato (Solanum tuberosum L.) Improves Processing Quality by Decreasing the Frequency of Sugar-End Defects

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e93381 ◽  
Author(s):  
Xiaobiao Zhu ◽  
Craig Richael ◽  
Patrick Chamberlain ◽  
James S. Busse ◽  
Alvin J. Bussan ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Gene Silencing ◽  
Solanum Tuberosum L ◽  
Processing Quality ◽  
Vacuolar Invertase ◽  
Invertase Gene ◽  
Sugar End

scholarly journals Primer Design for Isolation of Sucrose:Sucrose 1-Fructosyltransferase (1-SST) Gene from Gembili (Dioscorea esculenta)

2020 ◽  
Author(s):  
Gili Heriawan ◽  
Dellamartha Anjani ◽  
Putu Virgina Partha Devanthi ◽  
Rahmat Azhari Kemal ◽  
Priscilla Jessica
Keyword(s):  
Gene Sequence ◽  
Primer Design ◽  
The Other ◽  
Whole Genome Sequence ◽  
Its Sequence ◽  
Chromosome 6 ◽  
Genomic Information ◽  
Dioscorea Rotundata ◽  
Vacuolar Invertase ◽  
Invertase Gene

Recent studies have reported the presence of inulin, a prebiotic polysaccharide, in gembili (Dioscorea esculenta). Sucrose:sucrose 1-fructosyltransferase (1-SST) is an enzyme that catalyzes the first step of inulin biosynthesis. The identification of this enzyme would be the foundation to improve the yield of inulin in gembili; to modify 1-SST gene for this purpose, its sequence must first be determined. This study aimed to design primers to isolate 1-SST gene from D. esculenta. The primers were designed by using the whole-genome sequence of Dioscorea rotundata due to the lack of genomic information on D. esculenta. Sequences from chromosome 6 and 11 were used as the template of primer design in which four pairs of primers were selected. Amplification products with expected size were gel-purified, then the targets were sequenced and analyzed in-silico. As a result, one of the primer pairs had successfully isolated vacuolar invertase gene, which is closely related to 1-SST gene. On the other hand, the other primer pairs showed either negative or false-positive result. Using the current strategy, 1-SST gene sequence from D. esculenta has not been successfully isolated, thus other approaches in primer design should be considered in further studies. Keywords: 1-SST, Dioscorea esculenta, Gembili, Inulin, Primer design

Promoter regions of potato vacuolar invertase gene in response to sugars and hormones

2013 ◽  
Vol 69 ◽  
pp. 9-16 ◽  
Author(s):  
Yongbin Ou ◽  
Botao Song ◽  
Xun Liu ◽  
Conghua Xie ◽  
Meng Li ◽  
...  
Keyword(s):  
Promoter Regions ◽  
Vacuolar Invertase ◽  
Invertase Gene

Разработка и тестирование универсальных праймеров для ПЦР-амплификации генов-ортологов β-фруктофуранозидазы (Pain-1) у видов и сортов картофеля

2018 ◽  
Author(s):  
E.O. Shmelkova ◽  
M.A. Slugina ◽  
A.A. Meleshin ◽  
E.V. Romanova
Keyword(s):  
Solanum Tuberosum ◽  
Wild Species ◽  
Starch Synthesis ◽  
Full Length ◽  
Universal Primers ◽  
Potato Tubers ◽  
Vacuolar Invertase ◽  
Reserve Carbohydrate ◽  
Cold Induced Sweetening ◽  
Design And Testing

Работа посвящена разработке и тестированию универсальных праймеров для ПЦР-амплификации полноразмерных генов-ортологов β-фруктофуранозидазы (кислой вакуолярной инвертазы) у видов и сортов картофеля (Solanum tuberosum). Крахмал – основной источник энергии и резервный углевод, накапливающийся в амилопластах клубней. Образовавшаяся в результате фотосинтеза молекула глюкозы при реакции с фруктозой образует сахарозу – основную транспортную форму углеводов в растении. В клубни сахароза доставляется по флоэме (апопластный путь), где в межклеточном пространстве расщепляется до глюкозы и фруктозы, которые затем проникают в клетки паренхимы. Глюкоза служит в дальнейшем субстратом для синтеза крахмала в амилопластах. Однако при воздействии пониженных температур крахмал в клубнях картофеля разрушается до редуцирующих сахаров. Параллельно этому процессу идет ресинтез сахарозы до глюкозы и фруктозы за счет фермента кислой вакуолярной инвертазы (β-фруктофуранозидазы), кодируемой геном Pain-1. В совокупности эти процессы приводят к избыточному накоплению моносахаров в клубнях картофеля, так называемому холодовому осахариванию (cold-induced sweetening). При этом создаются условия для интенсивного образования меланоидинов, вызывающих потемнение мякоти картофеля, что значительно ухудшает товарное качество продукта. Таким образом, изучение гена Pain-1, кодирующего вакуолярную инвертазу, а именно, его идентификация и анализ структуры – важная задача, необходимая для поиска доноров, устойчивых к холодовому осахариванию. Первоочередная задача для этого – разработка и тестирование праймерных комбинаций, позволяющих амплифицировать полноразмерный ген у диких видов картофеля, а также сортов и линий культивируемого картофеля (S. tuberosum). В данной работе приведены результаты разработки и тестирования универсальных праймеров, с помощью которых можно амплифицировать как полноразмерные гены-ортологи, так и фрагменты гена Pain-1, а также подобраны оптимальные условия для проведения ПЦР реакции. Было разработано 6 праймерных комбинаций (PainF – PainR, PainF – Pain1exR, Pain1exF – Pain3exR, Pain2inF – Pain2inR, Pain3exF – Pain5exR, Pain5exF – PainR), среди которых комбинация PainF – PainR позволяла амплифицировать полноразмерный ген, остальные – внутренние и будут использованы в дальнейшем при секвенировании фрагментов исследуемого гена. Эти праймеры были успешно протестированы на 15 образцах, включающих представителей пяти дикорастущих видов картофеля (S. gourlay, S. chacoense, S. pinnatissectum, S. stoloniferum, S. vernei) и десяти сортов российской и зарубежной селекции (Гала, Ласунок, Ред Скарлетт, Рассет Бербанк, Мирас, Башкирский, Жуковский ранний, Матушка, Елизавета, Сударыня).The purpose of research is design and testing of universal primers for PCR amplification of full-length-fructofuranozidase orthologs genes (acid vacuolar invertase) in wild species and potato (Solanum tuberosum) varieties. Starch is the main source of energy and a reserve carbohydrate, that accumulates in tubers amyloplasts. Glucose molecule, produced by photosynthesis, reacts with fructose and forms sucrose, which is the main transport type of carbohydrates in the plant. In the tuber, sucrose is delivered via phloem (apoplast), where it splits into glucose and fructose, which then go to the parenchyma cells. Glucose is a further substrate for the starch synthesis in amyloplasts. However, low temperatures influence on potato tubers leads to starch break down to reducing sugars. In parallel to this process there is happens resynthesis of sucrose to glucose and fructose by acid vacuolar invertase enzyme (β-fructofuranosidase) encoded by Pain-1 gene. Together, these processes lead to an excessive accumulation of monosaccharides in potato tubers. This process also called as cold-induced sweetening. It creates conditions for the intensive formation of melanoidins, which cause a potato tubers darkening, which considerably impairs the commercial quality of the product. Thus, the study Pain-1 gene that encodes the vacuolar invertase (its identification and structure analysis) is an important task required for the search of donors resistant to cold-induced sweetening. The primary task for this is the design and testing of primer combinations that allow to amplify the full-length gene in wild potato species, varieties and lines of cultivated potato. In this work, we develop and test universal primers, that can amplify both full-length orthologs and fragments of the Pain-1 gene, and also select the optimal conditions for carrying out the PCR reaction. Summary. The purpose of research is design and testing of universal primers for PCR amplification of full-length-fructofuranozidase orthologs genes (acid vacuolar invertase) in wild species and potato (Solanum tuberosum) varieties. Starch is the main source of energy and a reserve carbohydrate, that accumulates in tubers amyloplasts. Glucose molecule, produced by photosynthesis, reacts with fructose and forms sucrose, which is the main transport type of carbohydrates in the plant. In the tuber, sucrose is delivered via phloem (apoplast), where it splits into glucose and fructose, which then go to the parenchyma cells. Glucose is a further substrate for the starch synthesis in amyloplasts. However, low temperatures influence on potato tubers leads to starch break down to reducing sugars. In parallel to this process there is happens resynthesis of sucrose to glucose and fructose by acid vacuolar invertase enzyme (β-fructofuranosidase) encoded by Pain-1 gene. Together, these processes lead to an excessive accumulation of monosaccharides in potato tubers. This process also called as cold-induced sweetening. It creates conditions for the intensive formation of melanoidins, which cause a potato tubers darkening, which considerably impairs the commercial quality of the product. Thus, the study Pain-1 gene that encodes the vacuolar invertase (its identification and structure analysis) is an important task required for the search of donors resistant to cold-induced sweetening. The primary task for this is the design and testing of primer combinations that allow to amplify the full-length gene in wild potato species, varieties and lines of cultivated potato. In this work, we develop and test universal primers, that can amplify both full-length orthologs and fragments of the Pain-1 gene, and also select the optimal conditions for carrying out the PCR reaction. In total 6 primer combinations were designed (PainF - PainR, PainF - Pain1exR, Pain1exF - Pain3exR, Pain2inF - Pain2inR, Pain3exF - Pain5exR, Pain5exF - PainR), where PainF - PainR primer combination allowed to amplify a full-sized gene, the rest are internal and will be used in the further fragments sequencing of the β-fructofuranosidase gene. These primers were successfully tested on 15 samples, including five wild species of potato (S. gourlay, S. chacoense, S. pinnatissectum, S. stoloniferum, S. vernei) and ten varieties of Russian and foreign breeding (Gala, Lasunok, Red Scarlet , Rasset Burbank, Miras, Bashkirsky, Zhukovsky ranniy, Matushka, Elizaveta, Sudaryna).

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