scholarly journals Primer Design for Isolation of Sucrose:Sucrose 1-Fructosyltransferase (1-SST) Gene from Gembili (Dioscorea esculenta)

2020 ◽  
Author(s):  
Gili Heriawan ◽  
Dellamartha Anjani ◽  
Putu Virgina Partha Devanthi ◽  
Rahmat Azhari Kemal ◽  
Priscilla Jessica
Keyword(s):  
Gene Sequence ◽  
Primer Design ◽  
The Other ◽  
Whole Genome Sequence ◽  
Its Sequence ◽  
Chromosome 6 ◽  
Genomic Information ◽  
Dioscorea Rotundata ◽  
Vacuolar Invertase ◽  
Invertase Gene

Recent studies have reported the presence of inulin, a prebiotic polysaccharide, in gembili (Dioscorea esculenta). Sucrose:sucrose 1-fructosyltransferase (1-SST) is an enzyme that catalyzes the first step of inulin biosynthesis. The identification of this enzyme would be the foundation to improve the yield of inulin in gembili; to modify 1-SST gene for this purpose, its sequence must first be determined. This study aimed to design primers to isolate 1-SST gene from D. esculenta. The primers were designed by using the whole-genome sequence of Dioscorea rotundata due to the lack of genomic information on D. esculenta. Sequences from chromosome 6 and 11 were used as the template of primer design in which four pairs of primers were selected. Amplification products with expected size were gel-purified, then the targets were sequenced and analyzed in-silico. As a result, one of the primer pairs had successfully isolated vacuolar invertase gene, which is closely related to 1-SST gene. On the other hand, the other primer pairs showed either negative or false-positive result. Using the current strategy, 1-SST gene sequence from D. esculenta has not been successfully isolated, thus other approaches in primer design should be considered in further studies. Keywords: 1-SST, Dioscorea esculenta, Gembili, Inulin, Primer design

ALLELE DIVERSITY FOR ACID VACUOLAR INVERTASE GENE Pain-1 FRAGMENT IN POTATO (Solanum tuberosum L.) VARIETIES AND LINES

2018 ◽  
Vol 53 (1) ◽  
pp. 132-139
Author(s):  
M.A. Slugina ◽  
◽  
E.O. Shmelkova ◽  
A.A. Meleshin ◽  
E.Z. Kochieva ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Vacuolar Invertase ◽  
Invertase Gene

scholarly journals Suppression of the Vacuolar Invertase Gene Prevents Cold-Induced Sweetening in Potato

2010 ◽  
Vol 154 (2) ◽  
pp. 939-948 ◽  
Author(s):  
Pudota B. Bhaskar ◽  
Lei Wu ◽  
James S. Busse ◽  
Brett R. Whitty ◽  
Andy J. Hamernik ◽  
...  
Keyword(s):  
Vacuolar Invertase ◽  
Invertase Gene ◽  
Cold Induced Sweetening

Arcobacter vandammei sp. nov., isolated from the rectal mucus of a healthy pig

2021 ◽  
Vol 71 (11) ◽  
Author(s):  
Pieter-Jan Kerkhof ◽  
Stephen L. W. On ◽  
Kurt Houf
Keyword(s):  
Gene Sequence ◽  
Whole Genome Sequence ◽  
Rrna Gene ◽  
Phenotypic Analysis ◽  
Sequence Comparisons ◽  
Content Type ◽  
Link Type ◽  
Intestinal Mucus ◽  
Protein Encoding

A study on the polyphasic taxonomic classification of an Arcobacter strain, R-73987T, isolated from the rectal mucus of a porcine intestinal tract, was performed. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain could be assigned to the genus Arcobacter and suggested that strain R-73987T belongs to a novel undescribed species. Comparative analysis of the rpoB gene sequence confirmed the findings. Arcobacter faecis LMG 28519T was identified as its closest neighbour in a multigene analysis based on 107 protein- encoding genes. Further, whole-genome sequence comparisons by means of average nucleotide identity and in silico DNA–DNA hybridization between the genome of strain R-73987T and the genomes of validly named Arcobacter species resulted in values below 95–96 and 70  %, respectively. In addition, a phenotypic analysis further corroborated the conclusion that strain R-73987T represents a novel Arcobacter species, for which the name Arcobacter vandammei sp. nov. is proposed. The type strain is R-73987T (=LMG 31429T=CCUG 75005T). This appears to be the first Arcobacter species recovered from porcine intestinal mucus.

Roles played by invertase and gene expression in the development of the horn-shaped gall on leaves of Rhus chinensis

2017 ◽  
Vol 44 (12) ◽  
pp. 1160 ◽  
Author(s):  
Zhen-Yuan Ruan ◽  
Xiao-Ming Chen ◽  
Pu Yang ◽  
Bing-Yi Wang
Keyword(s):  
Cell Wall ◽  
Invertase Activity ◽  
Activity Level ◽  
Rapid Expansion ◽  
Sink Strength ◽  
Cell Wall Invertase ◽  
Vacuolar Invertase ◽  
Growing Period ◽  
Rhus Chinensis ◽  
Invertase Gene

The present study deals with the growth and development of the horn-shaped gall, which is induced by Schlechtendalia chinensis Bell. on leaves of Rhus chinensis Mill. The relationship between gall formers and their host plants was investigated by means of the activities of various invertases, the expressions of the cell wall invertase gene (INV2), and vacuolar invertase gene (INV3) during gall development. Our results show that the increase in the sink strength of the galls required cell wall invertase and vacuolar invertase, and that vacuolar invertase had a particular impact during the early development. In addition, vacuolar invertase activity was always significantly higher in galls than in leaves. However, ionically bound cell wall invertase showed a slightly significant increased activity level when compared with the leaves after galls had entered the fast growing period. This result indicates that vacuolar invertase is related to the rapid expansion of the galls, but ionically bound cell wall invertase is involved in the rapid growth of tissues. The enhanced activity of cell wall invertase and the expression of INV2 may be a plant response to a gall-induced stress. Cytoplasmic invertase that acts as a maintenance enzyme, or takes part in the production of secondary metabolites, was elevated when intracellular acid invertase activity decreased.

scholarly journals Sequence diversity within a subgroup of mouse immunoglobulin kappa chains controlled by the IgK-Ef2 locus.

1981 ◽  
Vol 154 (1) ◽  
pp. 146-155 ◽  
Author(s):  
C Lazure ◽  
W T Hum ◽  
D M Gibson
Keyword(s):  
Normal Mouse ◽  
Sequence Diversity ◽  
The Other ◽  
Light Chains ◽  
Chromosome 6 ◽  
Immunoglobulin Kappa ◽  
Mouse Immunoglobulin ◽  
Complete Sequencing ◽  
Polymorphic Bands ◽  
V Region

We previously showed that a chromosome 6 locus, IgK-Ef2, controls a pair of prominent bands in normal mouse light-chain isoelectric focusing profiles. Screening of myeloma light chains derived from BALB/c mice (an IgK-EF2 alpha strain) led to the identification of seven light chains cofocusing with the polymorphic bands controlled by IgK-Ef2. Complete sequencing of the variable (V) regions of four of the light chains indicates that they are all members of the same subgroup (Vk-1A) and they differ from one another by 1--3 substitutions. One of the protein differs from the prototype V-region sequence only in the deletion of a single residue at position 95 immediately preceding of J region. The other two differ from the protype V region by 3 (two framework [fr], one complementarity-determined [cdr]) and one (fr) residues, respectively. Complete V-region sequences of two closely related light chains derived from NZB mice (an IgK-Ef2b strain) indicate the NZB proteins are derived from a distinct Vk gene (Vk-1B), differing by four substitutions from the Vk-1A sequence. The results suggest that the IgK-Ef2 polymorphism may be a result of, at least in part, the loss of the gene(s) coding for the Vk-1A subgroups in IgK-Ef2b strains of mice. The nature of the sequence diversity found in the Vk-1A subgroup indicates that either it is coded by a repeated series of virtually identical genes or that somatic mutation of a single Vk-1A gene may give rise to substitutions in framework as well as cdr regions.

Two new species of the Phimochirus holthuisi complex from the Gulf of Mexico, supported by morphology, color, and genetics (Crustacea: Anomura: Paguridae)

Zootaxa ◽  
2019 ◽  
Vol 4683 (4) ◽  
pp. 531-551
Author(s):  
DARRYL L. FELDER ◽  
RAFAEL LEMAITRE ◽  
CATHERINE CRAIG
Keyword(s):  
New Species ◽  
Gulf Of Mexico ◽  
Continental Shelf ◽  
Gene Sequence ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
The Other ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Gene Sequence Data

Coloration, gene-sequence data (H3, 12s, 16s), and subtle features in morphology support the description of two new species, both formerly regarded to represent accepted variants of Phimochirus holthuisi s.l. While color in life consistently separates these species from P. holthuisi s.s. and from each other, morphological distinctions are subtle and less than absolute in small specimens, being based on ventral spine counts of walking leg dactyls and relative development of the superior crest on the major chela. Molecular phylogenetic analyses clearly support the separation of sister clades, representing two new species, from P. holthuisi s.s. as well as other congeners available for analysis. Both of the new species are presently known to occur widely throughout the northern Gulf of Mexico, though one occurs more commonly in the northeastern and southeastern Gulf, and may range as far south as Suriname. The other has been taken primarily in the northwestern Gulf, and is not known from outside Gulf waters. While both of the new species appear restricted to relatively deep subtidal waters of the continental shelf, Phimochirus holthuisi s.s. is instead more commonly found in shallow nearshore tropical waters on or near coral reefs. Previous literature reports of P. holthuisi usually represent, at least in part, one or both of these two new species. 

scholarly journals CsINV5, a tea vacuolar invertase gene enhances cold tolerance in transgenic Arabidopsis

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Wenjun Qian ◽  
Bin Xiao ◽  
Lu Wang ◽  
Xinyuan Hao ◽  
Chuan Yue ◽  
...  
Keyword(s):  
Cold Tolerance ◽  
Transgenic Arabidopsis ◽  
Vacuolar Invertase ◽  
Invertase Gene

Evolution of European Cockchafers (Melolonthinae: Scarabaeidae: Coleoptera): a morphological, molecular and chromosomal study of intra- and inter-specific variations

2011 ◽  
Vol 101 (3) ◽  
pp. 345-352 ◽  
Author(s):  
T. Giannoulis ◽  
A.-M. Dutrillaux ◽  
Z. Mamuris ◽  
O. Montreuil ◽  
C. Stamatis ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Chromosomal Polymorphism ◽  
Morphological Characters ◽  
The Other ◽  
Ancestral Karyotype ◽  
Intraspecific Polymorphism ◽  
Morphological Variations ◽  
Chromosomal Study ◽  
Sequence Study

AbstractIn cockchafers of the genus Melolontha, there is a marked intraspecific polymorphism for morphological characters, making some specimens of one species resemble another. A cytogenetic and molecular (mitochondrial COI gene sequence) study of typical and atypical forms of M. melolontha and M. hippocastani, captured at the same period and area, was performed. Karyotypes and haplotypes clearly characterize each taxon, placing atypical specimens in one or the other species unambiguously. This formally discards the role of hybridization in phenotypic resemblance, as usually proposed. Karyotypes and haplotypes were compared to those of M. pectoralis and Phyllophaga pleei, a more distantly related Melolonthinae, and some Dynastinae species, to reconstruct their ancestral karyotype. The karyotype of M. melolontha is the most derivative and that of P. pleei the most conserved among the Melolonthinae studied, which fits with the phylogeny established by COI gene analysis. Both karyotypes and COI haplotypes demonstrate the proximity of M. pectoralis and M. melolontha. The karyotype of M. melolontha is polymorphic, without relationship with morphological variations. Finally, the existence of similar morphological variations in different Melolontha species and chromosomal polymorphism in M. melolontha is discussed in relation with a network (reticulated) mode of speciation.

scholarly journals The outer segment serves as a default destination for the trafficking of membrane proteins in photoreceptors

2008 ◽  
Vol 183 (3) ◽  
pp. 485-498 ◽  
Author(s):  
Sheila A. Baker ◽  
Mohammad Haeri ◽  
Peter Yoo ◽  
Sidney M. Gospe ◽  
Nikolai P. Skiba ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Membrane Proteins ◽  
Outer Segment ◽  
The Other ◽  
Visual Signals ◽  
Its Sequence ◽  
Specific Targeting ◽  
New Material ◽  
Targeting Signals ◽  
Syntaxin 3

Photoreceptors are compartmentalized neurons in which all proteins responsible for evoking visual signals are confined to the outer segment. Yet, the mechanisms responsible for establishing and maintaining photoreceptor compartmentalization are poorly understood. Here we investigated the targeting of two related membrane proteins, R9AP and syntaxin 3, one residing within and the other excluded from the outer segment. Surprisingly, we have found that only syntaxin 3 has targeting information encoded in its sequence and its removal redirects this protein to the outer segment. Furthermore, proteins residing in the endoplasmic reticulum and mitochondria were similarly redirected to the outer segment after removing their targeting signals. This reveals a pattern where membrane proteins lacking specific targeting information are delivered to the outer segment, which is likely to reflect the enormous appetite of this organelle for new material necessitated by its constant renewal. This also implies that every protein residing outside the outer segment must have a means to avoid this “default” trafficking flow.

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