Background:
MiR-493 promotes the proliferation of prostate cancer (PC) cells by targeting
PHLPP2. We aimed to explore the relationship between miR-493 and autophagy in PC.
Methods:
qRT-PCR and western blotting were used to determine the mRNA levels and protein expression
of miR-493, PHLPP2, autophagy gene BECN1 and ATG7 in PC cells. The autophagy gene expression
was determined after PC cells transfected with miR-493 precursor or PHLPP2 precursor. Corresponding
changes of autophagy phenotype and PC cell function were also studied.
Results:
The mRNA levels and protein expression of miR-493, PHLPP2, BECN1 and ATG7 in PC
cells were significantly decreased in PC cells. Overexpression of miR-493 or PHLPP2 markedly upregulated
the expression levels of BECN1 and ATG7 in PC cells. Overexpression of miR-493 and
PHLPP2 markedly promoted autophagy, and inhibited the invasion and cloning formation of PC cells.
Conclusion:
MiR-493 is a potent inducer of cytotoxic autophagy that leads to prostate cancer inhibition
by regulating on PHLPP2.
Immune checkpoint B7‐H3 protein expression is associated with poor outcome and androgen receptor status in prostate cancer