MiR-493 Induces Cytotoxic Autophagy in Prostate Cancer Cells through Regulation on PHLPP2

Background: MiR-493 promotes the proliferation of prostate cancer (PC) cells by targeting PHLPP2. We aimed to explore the relationship between miR-493 and autophagy in PC. Methods: qRT-PCR and western blotting were used to determine the mRNA levels and protein expression of miR-493, PHLPP2, autophagy gene BECN1 and ATG7 in PC cells. The autophagy gene expression was determined after PC cells transfected with miR-493 precursor or PHLPP2 precursor. Corresponding changes of autophagy phenotype and PC cell function were also studied. Results: The mRNA levels and protein expression of miR-493, PHLPP2, BECN1 and ATG7 in PC cells were significantly decreased in PC cells. Overexpression of miR-493 or PHLPP2 markedly upregulated the expression levels of BECN1 and ATG7 in PC cells. Overexpression of miR-493 and PHLPP2 markedly promoted autophagy, and inhibited the invasion and cloning formation of PC cells. Conclusion: MiR-493 is a potent inducer of cytotoxic autophagy that leads to prostate cancer inhibition by regulating on PHLPP2.

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YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Genes & Genomics ◽

10.1007/s13258-021-01175-z ◽

2021 ◽

Author(s):

Weijian Li ◽

Gaohuang Chen ◽

Zhenyu Feng ◽

Baoyi Zhu ◽

Lilin Zhou ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Proliferation ◽

Cell Function ◽

Migration And Invasion ◽

Sequencing Analysis ◽

Prostate Cancer Cells ◽

Mrna Sequencing ◽

Qrt Pcr ◽

Patient Prognosis

Abstract Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Differentially expressed mRNAs were identified by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verified that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

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430: Modulation of Heat-Shock Protein 90 (HSP90) Client Protein Expression in Prostate Cancer Cells by a Novel Novobiocin Analog

The Journal of Urology ◽

10.1016/s0022-5347(18)32686-7 ◽

2006 ◽

Vol 175 (4S) ◽

pp. 140-140

Author(s):

Manlio A. Goetzl ◽

Brian S. Blagg ◽

Benjamin Cronk ◽

Len Neckers ◽

Jeffrey M. Holzbeierlein

Keyword(s):

Prostate Cancer ◽

Heat Shock ◽

Heat Shock Protein ◽

Protein Expression ◽

Cancer Cells ◽

Heat Shock Protein 90 ◽

Client Protein ◽

Prostate Cancer Cells ◽

Hsp90 Client Protein

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Phenethyl isothiocyanate suppresses inhibitor of apoptosis family protein expression in prostate cancer cells in culture and in vivo

The Prostate ◽

10.1002/pros.22457 ◽

2011 ◽

Vol 72 (10) ◽

pp. 1104-1116 ◽

Cited By ~ 16

Author(s):

Kozue Sakao ◽

Sudhakar Desineni ◽

Eun-Ryeong Hahm ◽

Shivendra V. Singh

Keyword(s):

Prostate Cancer ◽

Protein Expression ◽

Cancer Cells ◽

Inhibitor Of Apoptosis ◽

Phenethyl Isothiocyanate ◽

Prostate Cancer Cells ◽

Cells In Culture ◽

Family Protein

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SOX9 stands for a bio-marker for prostate cancer detection

10.21203/rs.3.rs-53227/v1 ◽

2020 ◽

Author(s):

Hao Zi ◽

Wen-Lin Tao ◽

Lei Gao ◽

Zhao-Hua Yu ◽

Xiao-Dong Bai ◽

...

Keyword(s):

Prostate Cancer ◽

Cancer Detection ◽

Roc Curve ◽

Operating Characteristic ◽

Diagnostic Value ◽

Quantitative Real Time Pcr ◽

Optimal Cutoff ◽

Qrt Pcr ◽

Incidence And Mortality ◽

The Relationship

Abstract Background Prostate cancer is one of common cancers around the world, and in our country the incidence and mortality of PCa are both increasing. More and more reports have revealed that SOX9 is involved in various human cancers. In this study, we aimed to explore the relationship between SOX9 expression and diagnostic value of PCa patients. Methods In this study, quantitative real-time PCR (qRT-PCR) was performed to determine the expression of SOX9 of the 131 PCa patients and 74 healthy volunteers. And receiver operating characteristic (ROC) curve was used to determine the diagnostic value of SOX9 for PCa patients. Results The results of qRT-PCR showed that the expression of serum SOX9 in PCa patients was higher than that in healthy controls (P < 0.05). And the expression of SOX9 was significantly associated with PSA (P = 0.001), differentiation (P = 0.000), and lymph node metastasis (P = 0.000). Besides, the area under the ROC curve (AUC) was 0.966 with the sensitivity of 93.2% and specificity of 87.8% respectively. The optimal cutoff value of SOX9 was 2.34. Conclusions Our results found that SOX9 is a novel oncogene for PCa, and may be a novel and effective biomarker for the diagnosis of patients with PCa.

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Glycidamide Promotes the Growth and Migratory Ability of Prostate Cancer Cells by Changing the Protein Expression of Cell Cycle Regulators and Epithelial-to-Mesenchymal Transition (EMT)-Associated Proteins with Prognostic Relevance

International Journal of Molecular Sciences ◽

10.3390/ijms20092199 ◽

2019 ◽

Vol 20 (9) ◽

pp. 2199

Author(s):

Titus Ime Ekanem ◽

Chi-Chen Huang ◽

Ming-Heng Wu ◽

Ding-Yen Lin ◽

Wen-Fu T. Lai ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Cycle ◽

Protein Expression ◽

Cancer Cells ◽

Cancer Patients ◽

Epithelial To Mesenchymal Transition ◽

Gene Signature ◽

Prostate Cancer Cells ◽

Mesenchymal Transition ◽

Prognostic Relevance

Acrylamide (AA) and glycidamide (GA) can be produced in carbohydrate-rich food when heated at a high temperature, which can induce a malignant transformation. It has been demonstrated that GA is more mutagenic than AA. It has been shown that the proliferation rate of some cancer cells are increased by treatment with GA; however, the exact genes that are induced by GA in most cancer cells are not clear. In the present study, we demonstrated that GA promotes the growth of prostate cancer cells through induced protein expression of the cell cycle regulator. In addition, we also found that GA promoted the migratory ability of prostate cancer cells through induced epithelial-to-mesenchymal transition (EMT)-associated protein expression. In order to understand the potential prognostic relevance of GA-mediated regulators of the cell cycle and EMT, we present a three-gene signature to evaluate the prognosis of prostate cancer patients. Further investigations suggested that the three-gene signature (CDK4, TWIST1 and SNAI2) predicted the chances of survival better than any of the three genes alone for the first time. In conclusion, we suggested that the three-gene signature model can act as marker of GA exposure. Hence, this multi-gene panel may serve as a promising outcome predictor and potential therapeutic target in prostate cancer patients.

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Expression of PSA-RP2, an alternatively spliced variant from the PSA gene, is increased in prostate cancer tissues but the protein is not secreted from prostate cancer cells

Biological Chemistry ◽

10.1515/bc.2010.043 ◽

2010 ◽

Vol 391 (4) ◽

Cited By ~ 8

Author(s):

Astrid K. Whitbread ◽

Tara L. Veveris-Lowe ◽

Ying Dong ◽

Olivia L. Tan ◽

Robert Gardiner ◽

...

Keyword(s):

Prostate Cancer ◽

Seminal Fluid ◽

Prostate Cancer Cells ◽

Qrt Pcr ◽

Prostate Cells ◽

Alternatively Spliced ◽

Pc3 Cells ◽

Variant Transcript ◽

Cancer Tissues ◽

Spliced Variant

Abstract PSA-RP2 is a variant transcript expressed from the PSA gene that is conserved in gorillas, chimpanzees and humans suggesting a particular relevance for this transcript in these primates. We demonstrated by qRT-PCR that PSA-RP2 is upregulated in prostate cancer compared with benign prostatic hyperplasia tissues. The PSA-RP2 protein was not detected in seminal fluid and was cytoplasmically localised but not secreted from LNCaP or transfected PC3 prostate cells, despite secretion from transfected Cos-7 and HEK293 kidney cell lines. PSA-RP2-transfected PC3 cells showed slightly decreased proliferation and increased migration towards PC3-conditioned medium that could suggest a functional role in prostate cancer.

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Relationship of ERCC1 genotype variant with mRNA expression and ERCC1 protein levels in advanced urothelial carcinoma (UC).

Journal of Clinical Oncology ◽

10.1200/jco.2013.31.6_suppl.260 ◽

2013 ◽

Vol 31 (6_suppl) ◽

pp. 260-260

Author(s):

Elizabeth A. Guancial ◽

Lillian Werner ◽

Joaquim Bellmunt ◽

Nikitas Nikitas ◽

Edward C. Stack ◽

...

Keyword(s):

Protein Expression ◽

Mrna Expression ◽

Excision Repair ◽

Predictive Biomarker ◽

Mrna Levels ◽

Nuclear Staining ◽

Protein Levels ◽

Tissue Arrays ◽

Platinum Based Chemotherapy ◽

The Relationship

260 Background: DNA repair factors may be predictive for response to chemotherapies that produce DNA damage. While low ERCC1 protein and mRNA levels have been reported as associated with improved outcomes in metastatic UC patients treated with platinum-based chemotherapy, the relationship between genotype, mRNA expression, and protein level is unknown. The ERCC1 germline 19007C>T single-nucleotide polymorphism (SNP) is functionally associated with reduced translation of ERCC1 mRNA. We investigated the relationship between ERCC1 germline SNP, ERCC1 tumor mRNA and protein expression, in a cohort of patients with advanced UC who received first-line, platinum-based chemotherapy. Methods: A cohort of clinically annotated, uniformly-treated advanced UC patients with FFPE primary tumor tissue available was identified through the Hellenic cooperative Oncology Group (HECOG) (N=93). Genomic DNA extraction, nested PCR, and restriction fragment length polymorphism techniques for the 19007C>T SNP were performed to identify C/C, C/T and T/T genotypes. ERCC1 mRNA expression was interrogated using Nanostring nCounter profiling. IHC analysis was performed on tissue arrays using an ERCC1 antibody. Percent of positive nuclear staining was categorized as quartiles using previously identified cut-points. Results: ERCC1 C/T genotype was identified in 30/61 samples (49%) and T/T in 14/61 samples (23%). In 54 patients with both SNP and mRNA data available, T/T genotype was associated with the highest level of mRNA expression, followed by the C/T genotype (p=0.04). Neither ERCC1 genotype (N=44) nor ERCC1 mRNA expression (N=54) was associated with ERCC1 protein expression as measured by IHC (p=0.52 and p=0.13, respectively). Conclusions: ERCC1 19007C>T is associated with increased ERCC1 mRNA expression. However, neither genotype nor mRNA are surrogates for ERCC1 protein detected by IHC in advanced UC tumors. This suggests that while genotype influences mRNA expression of ERCC1, the use of the nucleotide excision repair pathway as a predictive biomarker of platinum-sensitivity may be more complex than previously appreciated and require the integrative use of proteomics, genomics and epigenomics.

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LncRNA PART1 modulates toll-like receptor pathways to influence cell proliferation and apoptosis in prostate cancer cells

Biological Chemistry ◽

10.1515/hsz-2017-0255 ◽

2018 ◽

Vol 399 (4) ◽

pp. 387-395 ◽

Cited By ~ 30

Author(s):

Ming Sun ◽

Donghua Geng ◽

Shuqiang Li ◽

Zhaofu Chen ◽

Wenyan Zhao

Keyword(s):

Prostate Cancer ◽

Cell Proliferation ◽

Cancer Cells ◽

Tunel Staining ◽

Toll Like Receptor ◽

Prostate Cancer Cells ◽

Chain Reactions ◽

Polymerase Chain Reactions ◽

Qrt Pcr ◽

Proliferation And Apoptosis

AbstractWe investigated thoroughly the effect of lncRNA PART1 on prostate cancer cells proliferation and apoptosis, through regulating toll-like receptor (TLR) pathways. LncRNA PART1 expression was also examined by quantitative real-time polymerase chain reactions (qRT-PCR) in human tissues and the cells lines LNCaP and PC3. After transfection with si-PART1 or control constructs, the cell viability was measured by MTS and colony formation assays. In addition, the apoptosis rate of the prostate cancer cells was validated by TUNEL staining. Relationships between lncRNA PART1 expression and TLR pathway genes were demonstrated by qRT-PCR and Western blotting. High levels of lncRNA PART1 expression were correlated with advanced cancer stage and predication of poor survival. LncRNA PART1 levels was increased in PCa cells treated with 5α-dihydrotestosterone (DHT), confirming PART1 was directly induced by androgen. Moreover, down-regulation of lncRNA PART1 inhibited prostate cancer cell proliferation and accelerated cell apoptosis. In addition, lncRNA PART1 induced downstream genes expression in TLR pathways includingTLR3,TNFSF10andCXCL13to further influence prostate cancer cells, indicating its carcinogenesis on prostate cancer. LncRNA PART1 promoted cell proliferation ability and apoptosis via the inhibition of TLR pathways in prostate cancer. LncRNA PART1 could hence be considered as a new target in the treatment of prostate cancer.

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