Flow cytometry analysis of early DNA content changes in human and monkey cells following infection with simian virus 40

Lippia lacunosa is a Brazilian savanna plant that belongs to the Verbenaceae family. It has been used in folk medicine as a treatment for different diseases. This species represents an endangered Brazilian medicinal plant, and this is the first report documenting a reliable protocol for the in vitro propagation and regeneration of L. lacunosa. Axenic explants were cultivated in MS medium containing different concentrations of naphthalene acetic acid (NAA) to induce root growth. The mean shoot length and the number of roots were highest with 0.06 mg·L-1 NAA. The highest number of buds in shoot regeneration was induced with 2 mg·L-1 6-benzylaminopurine (BA). To obtain a long-term culture, the dwarf shoots were elongated on MS media containing 0.5 mg·L-1 BA alternated with MS containing 2 mg·L-1 BA every 40 days. In the present protocol, the long-term shoots retained the ability to root even after long periods of BA treatment. In addition, we evaluated the nuclear DNA content and ploidy levels, including the occurrence of endopolyploidy, in long-term micropropagated plant leaves using flow cytometry analysis. The plants propagated in vitro over several years possessed nuclear DNA contents ranging from 2.940 to 3.095 pg, and no differences in DNA content were found among in vitro plants or between these plants and the control (L. lacunosa from a greenhouse with a DNA content of 3.08 pg). The flow cytometry analysis also demonstrated that there was no polyploidization. The present study will be useful for biotechnological approaches and provides the first estimate of the nuclear DNA content of this species using flow cytometry.

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Correlation of DNA content, p53, T antigen, and V antigen in simian virus 40-infected human diploid cells

Cytometry ◽

10.1002/cyto.990100212 ◽

1989 ◽

Vol 10 (2) ◽

pp. 205-213 ◽

Cited By ~ 21

Author(s):

Judith Laffin ◽

David Fogleman ◽

John M. Lehman

Keyword(s):

Dna Content ◽

Simian Virus 40 ◽

Simian Virus ◽

T Antigen ◽

V Antigen ◽

Human Diploid Cells ◽

Diploid Cells

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Flow Cytometry Analysis in Voided Urine of Patients with Bladder Cancer

Urologia Journal ◽

10.1177/039156039306000207 ◽

1993 ◽

Vol 60 (2) ◽

pp. 162-166

Author(s):

A. Lotto ◽

G. Carluccio ◽

A. Calisti ◽

A. Disperati ◽

E. Capuzzo ◽

...

Keyword(s):

Flow Cytometry ◽

Bladder Cancer ◽

Quantitative Evaluation ◽

Dna Content ◽

Urine Cytology ◽

Urinary Cytology ◽

Flow Cytometry Analysis ◽

Excellent Correlation ◽

First Diagnosis

Flow cytometry is known to be able to give a quantitative evaluation of the DNA of cellular populations (grade of ploidy), as well as to estimate the percentages of phases (S + G2M) providing useful information about the pathology in question and its aggressivity. This method has been applied in diagnosing patients with bladder cancer, using their voided urine and comparing with urine cytology. Our data, from 59 patients, indicate flow cytometry utility in diagnosing bladder cancer; in fact there is an excellent correlation between the urinary cytology and the DNA content in cytometry which increases in higher grade bladder cancer. The sensitivity of CFM is in the range of 92% to 94%, and is superior to that of conventional voided urine cytology (range 64% to 84%). It is felt that cytofluorometric analysis permits a reliable evaluation of voided urine, not only at first diagnosis, but especially during follow-up.

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Accumulation of cells with 4N DNA content at nonpermissive temperature in rat embryo diploid cells transformed bytsA mutant of simian virus 40

Journal of Cellular Physiology ◽

10.1002/jcp.1041270218 ◽

1986 ◽

Vol 127 (2) ◽

pp. 303-310 ◽

Cited By ~ 3

Author(s):

Atsuyuki Okuda ◽

Hideaki Tamura ◽

Hideo Shimura ◽

Genki Kimura

Keyword(s):

Dna Content ◽

Simian Virus 40 ◽

Simian Virus ◽

Nonpermissive Temperature ◽

Rat Embryo ◽

Diploid Cells

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Callus regeneration and polyploidy induction of Allium cepa L var. Bima Brebes using oryzalin

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/948/1/012043 ◽

2021 ◽

Vol 948 (1) ◽

pp. 012043

Author(s):

R Q A’ yun ◽

D Dinarti ◽

A Husni ◽

M Kosmiatin

Keyword(s):

Flow Cytometry ◽

Allium Cepa ◽

Ploidy Level ◽

Dna Content ◽

Callus Induction ◽

Consumer Preference ◽

Morphological Trait ◽

Flow Cytometry Analysis ◽

Allium Cepa L ◽

Callus Regeneration

Abstract Polyploidy induction could increase shallot bulb-size to raise consumer preference and local shallot productivity. The research aimed to obtain an effective method of polyploidy induction on callus of onion (Allium cepa) var. Bima Brebes. The experiment was consisted of two experimental steps, which were callus induction of onion and polyploid induction of the callus. A 1×1 cm callus was treated by two drops of oryzalin with concentrations 0, 25, 50, 75, 100, and 120 μM. The ploidy level was identified based on morphological trait, stomatal analysis and DNA content using a flow cytometry. The results showed callus diameter, number of green spots, and number of shoots were decreased with increasing oryzalin concentration. The planlet leaves regenerated from oryzalin treated callus were darker than that of control. The flow cytometry analysis showed that planlets with 75 μM oryzalin was tetraploid, had longer and wider stomata than that of the control.

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