Fast quantitative analysis of four tyrosine kinase inhibitors in different human plasma samples using three-way calibration- assisted liquid chromatography with diode array detection

2015 ◽  
Vol 38 (16) ◽  
pp. 2781-2788 ◽  
Author(s):  
Shou-Xia Xiang ◽  
Hai-Long Wu ◽  
Chao Kang ◽  
Li-Xia Xie ◽  
Xiao-Li Yin ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Tyrosine Kinase ◽  
Human Plasma ◽  
Tyrosine Kinase Inhibitors ◽  
Kinase Inhibitors ◽  
Diode Array ◽  
Diode Array Detection ◽  
Plasma Samples ◽  
Array Detection

Quantitation of Cannabinoids in Cannabis Dried Plant Materials, Concentrates, and Oils Using Liquid Chromatography–Diode Array Detection Technique with Optional Mass Spectrometric Detection: Single-Laboratory Validation Study, First Action 2018.11

2019 ◽  
Vol 102 (6) ◽  
pp. 1822-1833 ◽  
Author(s):  
Lukas Vaclavik ◽  
Frantisek Benes ◽  
Marie Fenclova ◽  
Jiri Hricko ◽  
Ales Krmela ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Diode Array ◽  
Diode Array Detection ◽  
Intermediate Precision ◽  
Method Performance ◽  
Plant Materials ◽  
In Series ◽  
Array Detection ◽  
Single Laboratory

Abstract This paper describes a single-laboratory validation of a liquid chromatography–diode array detection (LC–DAD) method for quantification of 12 major cannabinoids in Cannabis dried plant materials, concentrates, and oils. The method met Standard Method Performance Requirements for quantitative analysis of cannabinoids in Cannabis concentrates and Cannabis dried plant materials. The LOQs were in the range 0.003–0.10% (w/w), depending on the analyte and matrix. Spike recoveries were between 96.7 and 101.3% with relative SDs (RSDs) ≤2.3%. Precision expressed as repeatability and intermediate precision was within 0.3–4.8 and 1.1–5.1%, respectively. The chromatographic separation conditions used in this versatile method are compatible with both DAD–UV and MS detection. During method validation, high-resolution quadrupole time-of-flight MS was employed as a secondary detector (connected in series to the LC–DAD instrument) to provide high confidence identification of target analytes and as a tool for monitoring other cannabinoids for which reference standards were not available. The obtained results demonstrate applicability of the method to quantitative analysis of important cannabinoids in dried plants, concentrates, and oils. Limited data were generated for a food matrix (Cannabis-containing cookies) using this method with LC coupled to a compact single quadrupole mass spectrometer.

scholarly journals Optimization and Validation of a novel Nebulizer-assisted Liquid Phase Microextraction Followed by HPLC-DAD for Diazinon Analysis in Plasma Samples

2019 ◽  
Vol 9 (4) ◽  
pp. 221-232
Author(s):  
Reza Mohammadzaheri ◽  
Mehdi Ansari Dogaheh ◽  
Maryam Kazemipour ◽  
Kambiz Soltaninejad
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Liquid Phase ◽  
High Performance ◽  
Diode Array ◽  
Diode Array Detection ◽  
Plasma Samples ◽  
Liquid Phase Microextraction ◽  
Array Detection ◽  
Extracting Solvent

Background: Diazinon is among the most prevalently used broad-spectrum organophosphates insecticides. Diazinon toxicity depends on its blood concentration. The current study aimed to extract and determine diazinon in plasma samples using a new Nebulizer -Assisted Liquid-Phase Microextraction followed by High-Performance Liquid Chromatography with Diode-Array Detection (NALPME-HPLC-DAD).Methods: Several effective parameters, including the type and volume of extracting solvent, pH, surfactant, salt amount, and nebulizing, were evaluated and optimized to find the best condition for the extraction and determination of diazinon in plasma samples using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD). Additionally, the Plackett-Burman design was employed in preliminary experiments to screen the most appropriate parameters. Furthermore, we selected a central composite design to determine the best experimental conditions in NALPME-HPLC-DAD. Results: In an optimum condition, 412 μL of toluene (as extracting solvent) and nebulizing with nitrogen gas as dispersing and emulsification, sodium lauryl sulfate (2.8% w/v) and 100μL sodium chloride (1.5% w/v) in pH 8.1 were selected. The standard calibration curves for diazinon were linear with the concentration range of 0.5–4 µg/mL with a correlation coefficient of 0.9992. The Limit Of Detection (LOD) and Limit Of Quantification (LOQ) for diazinon were 0.123 µg/mL and 0.372 µg/mL, respectively.Conclusion: The proposed method was simple, accurate, precise, and sensitive for analyzing diazinon in the plasma samples. This method can be used for analyzing plasma diazinon concentrations in acute poisoning cases in clinical and forensic toxicology analyses.

Quantitation of Cannabinoids in Cannabis Dried Plant Materials, Concentrates, and Oils Using Liquid Chromatography–Diode Array Detection Technique with Optional Mass Spectrometric Detection: Single-Laboratory Validation Study, First Action 2018.11

2019 ◽  
Vol 102 (6) ◽  
pp. 1822-1833
Author(s):  
Lukas Vaclavik ◽  
Frantisek Benes ◽  
Marie Fenclova ◽  
Jiri Hricko ◽  
Ales Krmela ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Diode Array ◽  
Diode Array Detection ◽  
Intermediate Precision ◽  
Method Performance ◽  
Plant Materials ◽  
In Series ◽  
Array Detection ◽  
Single Laboratory

This paper describes a single-laboratory validation of a liquid chromatography–diode array detection (LC–DAD) method for quantification of 12 major cannabinoids in Cannabis dried plant materials, concentrates, and oils. The method met Standard Method Performance Requirements for quantitative analysis of cannabinoids in Cannabis concentrates and Cannabis dried plant materials. The LOQs were in the range 0.003–0.10% (w/w), depending on the analyte and matrix. Spike recoveries were between 96.7 and 101.3% with relative SDs (RSDs) ≤2.3%. Precision expressed as repeatability and intermediate precision was within 0.3–4.8 and 1.1–5.1%, respectively. The chromatographic separation conditions used in this versatile method are compatible with both DAD–UV and MS detection. During method validation, high-resolution quadrupole time-of-flight MS was employed as a secondary detector (connected in series to the LC–DAD instrument) to provide high confidence identification of target analytes and as a tool for monitoring other cannabinoids for which reference standards were not available. The obtained results demonstrate applicability of the method to quantitative analysis of important cannabinoids in dried plants, concentrates, and oils. Limited data were generated for a food matrix (Cannabis-containing cookies) using this method with LC coupled to a compact single quadrupole mass spectrometer.

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