Rapid characterization and identification of the chemical constituents and rat metabolites of Deng-Zhan-Xi-Xin injection using ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

Lobelia chinensis is a kind of herbal medicine widely distributed and used in Asia. The chemical components of this herb, however, have not been well studied until now. Lobeline, as an essential and famous bioactive compound in Lobelia genus, has been assumed to be present in L. chinensis. In order to ascertain its presence and, more importantly, proper use of this herb, chemical profiling this herb with highly sensitive and high-resolution analytical mass spectrometry was applied. In this study, high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC/Q-TOF MS) method was employed to systematically profile the chemical constituents of L. chinensis for the first time. Comparative chemical profiling study of L. chinensis and Lobelia inflata was also conducted to provide evidence whether lobeline is present or not. Piperidine alkaloids except for lobeline, alkaloid-lignan hybrids, flavonoids, polyacetylenes, nonanedioic acid, and some new phytochemicals were successfully identified in L. chinensis simultaneously. Comparing to the chemical profiles of L. inflata, lobeline was found to be absent in L. chinensis. All of the secondary metabolites in L. chinensis were determined with the HPLC/Q-TOF MS method. The absence of lobeline in L. chinensis was confirmed after this extensive study.

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Separation and analysis of flavonoid chemical constituents in flowers of Juglans regia L. by ultra-high-performance liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2018.11.029 ◽

2019 ◽

Vol 164 ◽

pp. 734-741 ◽

Cited By ~ 3

Author(s):

Mengmeng Yan ◽

Meng Chen ◽

Fei Zhou ◽

Desheng Cai ◽

Hua Bai ◽

...

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Chemical Constituents ◽

Juglans Regia ◽

Time Of Flight ◽

Flight Mass Spectrometry

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Rapid Screening and Identification of Chemical Constituents From Ophiopogon japonicus by High-Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

Journal of Chromatographic Science ◽

10.1093/chromsci/bmaa029 ◽

2020 ◽

Vol 58 (7) ◽

pp. 641-650

Author(s):

Yun Ling ◽

Yihua Tang ◽

Yuanyuan Xu ◽

Qing Zhang ◽

Chao Zhang ◽

...

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Electrospray Ionization ◽

High Performance ◽

Chemical Constituents ◽

Time Of Flight ◽

Steroidal Saponins ◽

Flight Mass Spectrometry ◽

Ophiopogon Japonicus

Abstract Ophiopogon japonicus (Thunb.) Ker-Gawl (Liliaceae), which is named as “Maidong” in China, is widely used in traditional Chinese medicine for treating fever, cough, inflammation, epistaxis, constipation, respiratory disease and gastrointestinal disorders. However, the systematic analysis of chemical constituents of O. japonicus has not been well established because of the complexity and trace. In this paper, an effective and reliable high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry method was successfully developed to separate and identify the chemical constituents from O. japonicus. As a result, a total of 30 compounds including steroidal saponins, homoisoflavonoids, allylbenzene, cholest and cryptomeridiol were screened or tentatively identified. Of them, three new steroidal saponins were found and tentatively characterized in O. japonicus. This study provides a meaningful material basis for further quality control and pharmacological research.

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